Experience

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  CellResearch Corporation

  • Singapore, Singapore

  • Scientific Officer

    • Jul 2023 - Present
    • Singapore, Singapore

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  Mechanobiology Institute

  • Singapore, Singapore

  • Final Year Student

    • Jul 2022 - May 2023
    • Singapore, Singapore

    The project aims to understand how ECM deregulation directly affects ovarian function.- Isolating primary granulosa cells (GCs) from sacrificial young mice- Fabrication of polyacrylamide (PA) hydrogels of varying stiffness + functionalisation of hydrogels with Sulfo-SANPAH- Seeding GCs onto PA hydrogels to mimic 2D environment + IF staining- Encapsulating GCs in Matrigel to mimic 3D environment + IF staining- Immunohistochemistry of young and old ovaries- Inducing senescence in primary GCs with varying concentrations of etoposide and varying durations- Observed for senescence with beta-galactosidase staining

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  Mechanobiology Institute

  • Singapore, Singapore

  • Undergraduate Research Opportunity Programme Student

    • Jan 2022 - May 2022
    • Singapore, Singapore

    · Sugar was used as sacrificial moulds to create microchannels to mimic microenvironment to betterunderstand cell migration in soft channels.· PDMS was fabricated and poured over sugar channels and sugar was dissolved in water.· Polyacrylamide hydrogels were fabricated to be used as soft environment.· MDS-MB-231 cancer cells seeding was done and imaging using confocal microscope was taken to observe if cells were migrating along the boundary created by the sugar channels.

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  CellResearch Corporation

  • Singapore, Singapore

  • Student Intern

    • Jun 2021 - Dec 2021
    • Singapore, Singapore

    · Aided in the packing of the lab during their moving from Ayer Rajah Crescent to Bukit Merah Central.· Trained in carrying out immunocytochemistry weekly.· Experienced in checking and verifying umbilical cord lining samples.· Exposed to some basics of cell culture techniques such as trypsinisation, cell counting and cellpassaging.· Introduced to flow cytometry and have experience performing it on mesenchymal stem cells.· Given the opportunity to participate in ad-hoc experiments such as adipogenesis and scratchexperiment.

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  Ministry of Education, Singapore (MOE)

  • Singapore

  • Substitute Teacher

    • Mar 2019 - May 2019
    • Singapore