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Daniel Lih is a seasoned scientist with 10+ years of experience in research and development, including stints at Curi Bio, University of Washington Bioengineering, National Yang Ming University, and Merica Group. He holds a Master of Science degree in Bioengineering and Biomedical Engineering from the University of Washington, and a Bachelor of Science degree from American School in Taichung. Lih has expertise in cellular and tissue imaging, high-throughput immunofluorescence, and data analysis.

Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Scientist
      • Sep 2022 - Present

      • Led and executed customer-focused projects by efficient human resources management, strategic experiment planning, and comprehensive slide deck preparation and presentation, contributing to business development and customer satisfaction.• Automated tasks related to cellular and tissue imaging, including high throughput immunofluorescence and cellular assays, live-cell imaging, and whole-section histology imaging, resulting in an increase in operational efficiency.• Pioneered the use of CellProfiler pipelines for analyzing myoblast purity, myofiber quantification, and other cellular assays, improving data reliability and processing speed.

    • Senior Research Associate
      • Sep 2021 - Sep 2022

      • Developed and managed a comprehensive imaging system/database, establishing a new standard for high-quality scientific imaging across the team.

    • Research Associate
      • Apr 2021 - Sep 2021

      • Established lab management systems including ordering and inventory, liquid nitrogen storage, and environmental monitoring for freezers, refrigerators, and incubators.• Implemented Standard Operating Procedures (SOPs), templates, and macros for new research processes and experiments, contributing to lab efficiency.

  • University of Washington Bioengineering
    • Seattle, Washington, United States
    • Research Scientist
      • Aug 2016 - Apr 2021
      • Seattle, Washington, United States

      • Developed immortalized human kidney endothelial cell lines, replacing the use of commercially available HUVEC and improving organ specificity in Ying's and collaborators' kidney research.• Achieved a pivotal role as the 'go-to' person for virus production, mastering skills in transfection and transduction.• Established efficient record-keeping systems, becoming a reliable point of contact for past colleagues seeking data from physical lab notebooks.• Characterized and analyzed genotypes and phenotypes of various cell lines under different conditions using techniques like flow cytometry, qRT-PCR, Western blot, ELISA, and immunofluorescent staining.• Successfully differentiated human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) into cardiomyocytes, endothelial cells, and kidney organoids.• Led the kinase activity assay project, using high-throughput imaging and analysis to screen the effects of over 30 kinases in human organ- specific endothelial cell lines.

  • University of Washington Bioengineering
    • Seattle, Washington, United States
    • Graduate Research Assistant
      • Mar 2012 - Jan 2016
      • Seattle, Washington, United States

      • Designed nanopatterned cantilever assays for cardiac preclinical screening.• Cultured NRVM, C2C12, and hESC-CMs.• Differentiated hESCs and iPSCs into cardiomyocyte using feeder-free method.• Analyzed images and videos using ImageJ and Matlab.• Fabricated biomaterial substrates using nanolithography and spincoating using PDMS and PLGA.

    • Collaboration Researcher
      • Jul 2013 - Aug 2013
      • Taipei City, Taiwan

  • Merica Group
    • Taichung City, Taiwan
    • Teaching Assistant
      • Jul 2011 - Sep 2011
      • Taichung City, Taiwan

Education

  • 2014 - 2016
    University of Washington
    Master of Science (M.S.), Bioengineering and Biomedical Engineering
  • 2010 - 2014
    University of Washington
    Bachelor of Science (BS), Bioengineering and Biomedical Engineering
  • 2007 - 2010
    American School in Taichung
    High School Degree

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Industry Focus. “Biotechnology”

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