Experience

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  Poseida Therapeutics, Inc.

  • San Diego, California, United States

  • Flow Cytometry Core Manager

    • Aug 2022 - Present
    • San Diego, California, United States

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  Novartis Institutes for BioMedical Research (NIBR)

  • Senior Scientist I

    • Feb 2021 - Aug 2022

    Specialized in FACS, FACS based high throughput screenings, microfluidics, and mass spec flow using Cytofs and IMC via Hyperion imager.Provided routine general maintenance on a wide array of instruments including BD LSRFortessas, BioRad Ze5/Yetis, Cytek Aurora, BD FACSAriaIIn NanoCellect Wolf, Fluidigm Cytof 2 and Hyperion IMC, and Sysmex XN-1000 hematology analyzer.Prepped cells for FACS based high-throughput multiplexed hybridoma discovery, performed cytometer set up and analysis on subsequent data.Designed high parameter fluorochrome panels for flow and spectral cytometry.Developed training regimens for users, aided in data acquisition and analysis via FlowJo.Experienced in a wide array of flow cytometry software including: Everest, FACSDiva, Wolfviewer, CyTOF, and FlowJo.Collaborated closely with multidisciplinary groups including Biology, Chemistry and Engineer to enable Scientific discovery.

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  Genomic Institute of the Novartis Research Foundation

  • Flow Cytometrist, Scientific Associate II

    • May 2018 - Feb 2021

    Daily maintenance of Flow Cytometers and cell sorters including BD Fortessas, BioRad Yeti/Ze5, Cytek Aurora, BD FACSAria and Nanocellect WOLF sorter. Additionally, maintained CyTOF and imaging Hyperion CyTOF. Designed experiments and panel design to optimize user experience and quality of data. Trained users on instrumentation and running samples. Regularly sorted cells for users on both FACSAria and NanoCellect sorters. Collaborated with Biologists and Engineers to conduct hybridoma antibody discovery through automated high throughput screens.

  • Scientific Associate II

    • Aug 2017 - May 2018

    As a team member of the Antibody Therapeutics Team I was responsible for testing antibody-drug conjugate candidates for the treatment of prevalent diseases with no current cure/treatment. Duties included; characterization of antibodies in various in vitro assays to determine whether the candidates should be pushed forward into in vivo studies or not. Research required collaboration between several disciplines and teams on how to best proceed and optimize lead candidates.

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  Wolkowicz Laboratory

  • San Diego State University

  • Graduate Research Associate

    • Jun 2014 - Jun 2017
    • San Diego State University

    Molecular Virology Laboratory that focuses on cleavage by viral and host proteases as novel targets for the development of therapeutics against HIV, Dengue Virus, West Nile Virus, Zika Virus, Hepatitis C Virus, and Chikungunya Virus.

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  Pacific GMP

  • Greater San Diego Area

  • Summer Intern

    • Jun 2014 - Aug 2014
    • Greater San Diego Area

    Following SOPs for general laboratory upkeep, gaining knowledge in good manufacturing practices