Bio
Experience
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United States
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Biotechnology Research
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1 - 100 Employee
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Principal Scientist, R&D
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Sep 2023 - Present
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Scientific Manager
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Apr 2019 - Sep 2023
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Senior Scientist
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Oct 2015 - Apr 2019
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Post Doctoral Fellow
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Oct 2014 - Sep 2015
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Bangalore
In my Post-doctoral fellowship, I am exploring about the biochemical studies of some of the mutants of HCM/DCM and its comparison with the wild type sarcomeric proteins (e.g. tropomyosin, troponin etc) by using various techniques like invitro motility assay, ATPase assay etc.
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NCBS-TIFR
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BANGALORE
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PhD SCHOLAR
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Oct 2009 - Sep 2014
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BANGALORE
Title of PhD Thesis: Role of Voltage Dependent Anion Channel (VDAC)-Hexokinase Interaction in programmed cell death. In my PhD thesis, I have explored about,1. Biophysical characterization of VDAC purified from different sources by using Circular dichroism (CD), liposome swelling assay and and Electrophysiological characterization by bilayer lipid membrane (BLM) technique. For the purification of VDAC proteins I used various molecular biological techniques like DNA manipulation, sub-cloning, PCR, agarose electrophoresis, protein purification by HPLC based size exclusion, ion exchange and affinity chromatography, western blot.2. Explored the VDAC-Hexokinase interaction in animal and plants systems. I have used various cell biological approach like sub-culturing of mammalian cell culture (HeLa, HEK, CHO, and NFF cells), plant cell culture (BY2 cell line), and immunological techniques like Immuno-precipitation, Immunostaining, immunoblotting. I have used many microscopy like Fluorescence, Confocal and Total Internal Reflection Fluorescence (TIRF) microscopy to determine the VDAC-Hexokinase interaction in these system.
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Indian Institute of Sciences (IISc) Bangalore
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Bengaluru Area, India
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Project Assistant
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Jun 2008 - Oct 2009
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Bengaluru Area, India
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Indian Institute of Science
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Bangalore
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Project Assistant
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Jul 2007 - Jun 2008
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Bangalore
Worked on “Genetics and Developmental Study on the Trisanku mutant of Dictyostelium discoideum”.
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Education
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2009 - 2014National Centre for Biological Sciences (NCBS)-TIFR, Bangalore,India
PhD, Biotechnology -
2005 - 2007Mysore University
Master degree: M.Sc, Biotechnology -
2005 - 2007Mysore University
Master degree: M.Sc, Biotechnology
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