José Matheus Camargo Bonatto
Senior Research Specialist at University of Illinois Chicago- Claim this Profile
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Inglês Full professional proficiency
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Francês Elementary proficiency
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Bio
Experience
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University of Illinois Chicago
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United States
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Higher Education
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700 & Above Employee
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Senior Research Specialist
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Apr 2023 - Present
Senior Research Specialist at the University of Illinois Chicago, working at the Research Resources Center at the Mass Spec Core Facility. I prepare samples and analyze biological samples for Metabolomics and Proteomics, both target and untarget, with different applications such as drug discovery, disease biomarkers, and signaling pathways, for example. I have knowledge and use the following instruments: AB Sciex Qtrap5500/6500 (MRM Quantitation), Agilent Q-TOF 6545 (Untargeted Metabolics/Lipidomics), Perkin Elmer NexIon 2000s ICP (Metal analysis, Speciation), Bruker MALDI-TOF Microflex (Accurate Molecular Weight), Thermo Orbitrap Velos Pro (Proteomics, PTM), Thermo Orbitrap QE-HF (Proteomics, PTM). Show less
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MBA USP/Esalq
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Brazil
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E-Learning Providers
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100 - 200 Employee
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Professor advisor
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Aug 2022 - Present
Advisor professor in the Project Management and People Management courses in the MBA/ESALQ program Advisor professor in the Project Management and People Management courses in the MBA/ESALQ program
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LaCTAD-UNICAMP
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Brazil
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Research Services
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1 - 100 Employee
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Senior Research Coordinator
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Jan 2020 - Present
Research Coordinator at the proteomics and metabolomics facility, working on the development of methods and routine in mass spectrometry. Extensive experience in SDS_PAGE, lysate, or extracellular vesicle preparation of biological samples for untarget proteomics, quantitative and comparative label-free, analysis of post-translational modifications, de novo sequencing. Recently, implementation of untarget metabolomics in different chromatographic methods and statistical comparisons. Working with platforms ACQUITY UPLC and XEVO G2 XS Q-TOF, Progenesis and UNIFI. Show less
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IonMedicine
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Brazil
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Medical Practices
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1 - 100 Employee
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Scientific Researcher
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May 2019 - Jan 2020
Specialist in mass spectrometry for the development of new analytical methods for medical examinations using target metabolomics with the Shimadzu triple-quadrupole LCMS 8060 equipment. Specialist in mass spectrometry for the development of new analytical methods for medical examinations using target metabolomics with the Shimadzu triple-quadrupole LCMS 8060 equipment.
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Universidade Estadual de Campinas
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Brazil
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Research Services
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700 & Above Employee
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Postdoctoral Fellowship
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Jan 2017 - May 2019
Post-doctoral researcher in organic chemistry, IQ-UNICAMP with GlaxoSmithKline (GSK, through Cersuschem, FAPESP). Responsible for Molecular biology and bioinformatics for cloning and expression of enzymes of pharmaceutical interest, synthesis, and Purification of small pharmaceutical molecules, GC-MS, Microbiology applied to organic chemistry, MALDI-TOF for identification of bacteria. Post-doctoral researcher in organic chemistry, IQ-UNICAMP with GlaxoSmithKline (GSK, through Cersuschem, FAPESP). Responsible for Molecular biology and bioinformatics for cloning and expression of enzymes of pharmaceutical interest, synthesis, and Purification of small pharmaceutical molecules, GC-MS, Microbiology applied to organic chemistry, MALDI-TOF for identification of bacteria.
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Institute of Biomedical Sciences - University of Sao Paulo
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Brazil
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Higher Education
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100 - 200 Employee
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Laboratory Analyst
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May 2016 - Dec 2016
Mastery in routine, operation, and maintenance of Mass spectrometer Equipment of the facility BIOMASS CEFAP-USP (Orbitrap Velos, Tri-Quadrupole Quantum, and MALDI-TOF/TOF Autoflex). Preparation of samples, development of new methods, management of the facility routine activities, discussion and targeting of results with customers. Mastery in routine, operation, and maintenance of Mass spectrometer Equipment of the facility BIOMASS CEFAP-USP (Orbitrap Velos, Tri-Quadrupole Quantum, and MALDI-TOF/TOF Autoflex). Preparation of samples, development of new methods, management of the facility routine activities, discussion and targeting of results with customers.
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Grupo Fleury
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Brazil
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Health, Wellness & Fitness
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700 & Above Employee
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Laboratory Analyst in Research and Development
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Apr 2015 - Apr 2016
It acts in the search for biomarkers of clinical interest with a proteomic and metabolomic approach. Mastery of expression and purification of proteins of clinical interest, development of methods in liquid chromatography and mass spectrometry, operation of Orbitrap Q-Exactive. Experience in routine in the chromatography, microbiology and immunology sector. Good laboratory practices, experience in R&D and Innovation management tools. It acts in the search for biomarkers of clinical interest with a proteomic and metabolomic approach. Mastery of expression and purification of proteins of clinical interest, development of methods in liquid chromatography and mass spectrometry, operation of Orbitrap Q-Exactive. Experience in routine in the chromatography, microbiology and immunology sector. Good laboratory practices, experience in R&D and Innovation management tools.
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Institute of Biomedical Sciences - University of Sao Paulo
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Brazil
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Higher Education
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100 - 200 Employee
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Laboratory Analyst
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Jul 2014 - Apr 2015
Mastery in routine, operation, and maintenance of Mass spectrometer Equipment of the facility BIOMASS CEFAP-USP (Orbitrap Velos, Tri-Quadrupole Quantum, and MALDI-TOF/TOF Autoflex). Preparation of samples, development of new methods, management of the facility routine activities, discussion and targeting of results with customers. Mastery in routine, operation, and maintenance of Mass spectrometer Equipment of the facility BIOMASS CEFAP-USP (Orbitrap Velos, Tri-Quadrupole Quantum, and MALDI-TOF/TOF Autoflex). Preparation of samples, development of new methods, management of the facility routine activities, discussion and targeting of results with customers.
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USP - Universidade de São Paulo
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Brazil
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Higher Education
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700 & Above Employee
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PhD in Biochemistry
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Apr 2010 - Oct 2014
Post-graduation strict sense in biochemistry and Molecular Biology, mastery of purification techniques and characterization of signaling pathways proteins by 2D-PAGE AND LC-MS/MS, tools of immunoassays as Western blot, Mammalian cell cultures, especially embryonic stem cell culture, cell transfection, genetic silation and PCR analysis in real time. Post-graduation strict sense in biochemistry and Molecular Biology, mastery of purification techniques and characterization of signaling pathways proteins by 2D-PAGE AND LC-MS/MS, tools of immunoassays as Western blot, Mammalian cell cultures, especially embryonic stem cell culture, cell transfection, genetic silation and PCR analysis in real time.
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JLA Argentina
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Argentina
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Research Services
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1 - 100 Employee
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Application Laboratory Manager
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Feb 2014 - Jul 2014
Development of methods, operation, and validation in Tri-Quadrupole Xevo TQD for identification and quantification of pesticides, aflatoxins and haloacetic acids in foods, like to Peanut Derivatives, vegetable oil, and drinking water. Laboratory routine of providing services in food and environmental, development of methods with accreditation (ABNT 17025:2005), knowledge of quality management, physicochemical Techniques, and technical training. Development of methods, operation, and validation in Tri-Quadrupole Xevo TQD for identification and quantification of pesticides, aflatoxins and haloacetic acids in foods, like to Peanut Derivatives, vegetable oil, and drinking water. Laboratory routine of providing services in food and environmental, development of methods with accreditation (ABNT 17025:2005), knowledge of quality management, physicochemical Techniques, and technical training.
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Escola Superior de Agricultura "Luiz de Queiroz"
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Brazil
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Higher Education
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300 - 400 Employee
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Master Degree Student
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Mar 2009 - Dec 2010
Post-graduation strict sense in genetics and breeding, mastery of purification techniques and characterization of plant development proteins by 2D-PAGE AND LC-MS/MS, plant culture model as Nicotiana tabacum and of industrial interest as Eucalyptus grandis, transformation and culture of vegetal tissues, analysis by optical and electron microscopy, monitoring by PCR in real time. Post-graduation strict sense in genetics and breeding, mastery of purification techniques and characterization of plant development proteins by 2D-PAGE AND LC-MS/MS, plant culture model as Nicotiana tabacum and of industrial interest as Eucalyptus grandis, transformation and culture of vegetal tissues, analysis by optical and electron microscopy, monitoring by PCR in real time.
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Escola Superior de Agricultura "Luiz de Queiroz"
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Brazil
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Higher Education
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300 - 400 Employee
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Iniciação Cientifica
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Feb 2003 - Dec 2007
Bachelor and graduated with scientific initiation in plant genetics in the study of biotic and abiotic stress in Eucalyptus grandis, in particular, water stress in partnership with pulp and Paper company. Use of techniques of purification and characterization of protein responses to stress by 2D-PAGE and LC-MS/MS, tools of the photochemical capacity of plants. Bachelor and graduated with scientific initiation in plant genetics in the study of biotic and abiotic stress in Eucalyptus grandis, in particular, water stress in partnership with pulp and Paper company. Use of techniques of purification and characterization of protein responses to stress by 2D-PAGE and LC-MS/MS, tools of the photochemical capacity of plants.
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Education
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Universidade de São Paulo
Licenciado e Bacharel em Ciências Biológicas, Biology/Biological Sciences, General