Amaury AOUAD
Technicien de recherche en biologie at institut de Biologie Valrose - iBV- Claim this Profile
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Bio
Experience
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institut de Biologie Valrose - iBV
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France
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Think Tanks
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1 - 100 Employee
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Technicien de recherche en biologie
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Mar 2022 - Present
Chargé de l'étude d'une protéine probablement impliquée dans le recrutement de composants de l'adhésion cellulaire. -Clonage moléculaire, -Gestion des commandes -Culture cellulaire primaire -Culture cellulaire de lignées immortalisées (HEK293T, TIFs) Chargé de l'étude d'une protéine probablement impliquée dans le recrutement de composants de l'adhésion cellulaire. -Clonage moléculaire, -Gestion des commandes -Culture cellulaire primaire -Culture cellulaire de lignées immortalisées (HEK293T, TIFs)
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Technicien de recherche en biologie
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Sep 2021 - Mar 2022
Remplacement de zootechnicien: -Génotypage animalier -Gestion des lignées de souris C57Bl/6 mutantes (gestion des registres souris, changement de cage, sevrage) -Gestion du laboratoire -Gestion des commandes -Gestion de l'hygiène et sécurité du laboratoire Remplacement de zootechnicien: -Génotypage animalier -Gestion des lignées de souris C57Bl/6 mutantes (gestion des registres souris, changement de cage, sevrage) -Gestion du laboratoire -Gestion des commandes -Gestion de l'hygiène et sécurité du laboratoire
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Master Thesis
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Jan 2021 - Jun 2021
The project was to: -Characterize a population of adrenocortical cancer cell line resistant to chemotherapy as a cancer stem cell-like cell line by identifying cancer stem cells properties such as high migration rate, increased invasiveness, protein expression of cancer stem cell markers -Understand the mechanism of endocytosis of the transporter highly expressed responsible for this chemotherapy resistance. The project was to: -Characterize a population of adrenocortical cancer cell line resistant to chemotherapy as a cancer stem cell-like cell line by identifying cancer stem cells properties such as high migration rate, increased invasiveness, protein expression of cancer stem cell markers -Understand the mechanism of endocytosis of the transporter highly expressed responsible for this chemotherapy resistance.
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Institut Curie
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France
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Hospitals and Health Care
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700 & Above Employee
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Master Thesis
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Feb 2021 - Feb 2021
Working on my internship to understand the mechanims of endocytosis of a transporter with a mechanism independent of clahtrins called Glycolipid-lectin mechamism discovered in this institute. I did special immunofluorescence assays to observe this mechanism in an adrenocortical cancer cell line. Working on my internship to understand the mechanims of endocytosis of a transporter with a mechanism independent of clahtrins called Glycolipid-lectin mechamism discovered in this institute. I did special immunofluorescence assays to observe this mechanism in an adrenocortical cancer cell line.
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LP2M - Université Côte d'Azur - CNRS UMR7370
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France
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Higher Education
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1 - 100 Employee
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Master Thesis Student
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Feb 2020 - Jun 2020
The project was to assess the control of the metabolism of induced pluripotent stem cells (iPSC) differentiated in Mesenchymal stromal cells through the PD1/PDL1 axis. For that, I: -proceeded to RT-qPCR analysis to identify mRNA coding for metabolic enzymes -performed Seahorse analysis in different conditions to assess the metabolism of this cells by modifying PD1/PDL1 axis with drugs -assessed with FACS (Fluorescence Activated Sorting Cells) the expression of transporter implied in metabolism I performed a small review to sum up the literature context of this project. Show less
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Education
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Université Côte d’Azur
Master's degree, Physio-pathology and Pharmacology -
Université Côte d’Azur
Bachelor's degree, Health and Science