Gabriel Mendívil Díez
TFM: Development of a DNA vaccine based on replicative RNA. at Cima Universidad de Navarra- Claim this Profile
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Inglés Full professional proficiency
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Castellano Native or bilingual proficiency
Topline Score
Bio
Credentials
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Sistema de Garantía Juvenil
Gente con Garantía JuvenilNov, 2022- Oct, 2024 -
Beca Banco Santander - Habilidades | Evolution Universidades
Banco SantanderApr, 2022- Oct, 2024 -
Capacitación de funciones A, B y C de experimentación animal para roedores.
Gobierno de NavarraJan, 2022- Oct, 2024 -
Curso: Riesgo Químico y Biológico en los laboratorios del CIMA
Cima Universidad de NavarraOct, 2021- Oct, 2024 -
International Workshop on Genomic Testing in Cancer: Facing a new era in personalized medicine.
Clínica Universidad de NavarraOct, 2021- Oct, 2024 -
Permiso de Conducción tipo B
Dirección General de TráficoOct, 2021- Oct, 2024 -
Las enfermedades tropicales desatendidas en el contexto de la pandemia COVID-19: impacto y guía
World Health OrganizationSep, 2021- Oct, 2024 -
Recursos de vacunas específicas frente a la COVID-19
World Health OrganizationSep, 2021- Oct, 2024 -
Cuarta revolución Industrial: Bioingeniería
LinkedInAug, 2021- Oct, 2024 -
Curso de Competencias digitales para profesionales (40 horas)
Google ActívateAug, 2021- Oct, 2024 -
Curso de Prevencion de Riesgos en el Laboratorio (25 horas)
Asociación EventEXAug, 2021- Oct, 2024 -
Curso de Productividad personal (40 horas)
Google ActívateAug, 2021- Oct, 2024 -
Desarrollar la creatividad en las organizaciones
LinkedInAug, 2021- Oct, 2024 -
La importancia de las habilidades de vida o soft skills
LinkedInAug, 2021- Oct, 2024 -
Liderazgo y trabajo en equipo
LinkedInAug, 2021- Oct, 2024 -
Jornadas online de transparencia en la experimentación animal
Universidade de Santiago de CompostelaJul, 2021- Oct, 2024 -
Diagnóstico por NGS en enfermedades raras
IlluminaJun, 2021- Oct, 2024 -
Edición del Genoma mediante CRISPR y células madre pluripotentes inducidas en la investigación de enfermedades genéticas (15 horas)
GenotipiaJun, 2021- Oct, 2024 -
Curso Entender el medio ambiente: Relación de las personas con el medio ambiente (3 ECTS).
Universidad de NavarraMay, 2021- Oct, 2024 -
Curso online: ‘’Divulga AECC: del laboratorio a la sociedad’’
Asociación Española Contra el Cáncer (AECC)Apr, 2021- Oct, 2024 -
Certificado Inglés nivel B2
EF Standard English Test (EF SET)Jan, 2021- Oct, 2024 -
Miembro Socio de la Asociación de Estudiantes de Biología de España - AEBE
Asociación de Estudiantes de Biología de España - AEBEJun, 2020- Oct, 2024 -
Primer Precolegiado
Colegio oficial de Biólogos de Navarra y La RiojaOct, 2019- Oct, 2024 -
Diploma Cetáceos del Golfo de Bizkaia: Curso Teórico (Biología, Conservación y Métodos de estudio) + Avistamiento en barco
TerrabiotaSep, 2019- Oct, 2024 -
Introduction to Conservation
National GeographicApr, 2019- Oct, 2024
Experience
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Cima Universidad de Navarra
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Spain
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Research Services
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100 - 200 Employee
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TFM: Development of a DNA vaccine based on replicative RNA.
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Sep 2021 - Present
Director: Dr. Puri Fortes. Co-director: Dr. Pablo Sarobe. Departments: Immunotherapy and Immunology and Gene Therapy. - The aim of this project was to design, produce and test a DNA vaccine platform (NB-PEPI-UM) based on Semliki Forest Virus replicative RNA, containing elements that enhance the 3 signals necessary for the activation of the immune response: a polyepitope (PEPI), an anti-PD-1 nanobody (NB), and Ubiquitin (U) and MHC trafficking (MITD; M) domains to improve antigen processing and presentation. A vector was also produced that would act in trans and encodes the soluble factors XCL1 and FLT3L. I demonstrated nanobody expression by Western blot and PEPI expression by immunizing mice and its antitumor effect in reducing tumor size in a mouse melanoma model and in quantifying IFN-γ produced by immunized mouse spelunocytes. - I performed more than 100 digests, ligation and cloning of plasmids in less than 3 months, also more than 25 Western blots, more than 20 PCRs, 10 ELISAS and ELISPOTs, I also maintained for 8 months several cell lines performing more than 30 transfections with lipofectamine and manipulated more than 100 mice, all this to have as a result an effective vaccine construct and allowed me to gain technical and analytical skills, organization in time managing resources and coordination of different experiments and teams of people. Show less
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Navarrabiomed, biomedical research centre
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Spain
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Research Services
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100 - 200 Employee
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Graduate Ad Honorem Internship in Molecular Pathology of Cancer Navarrabiomed (189 hours)
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Aug 2021 - Sep 2021
Tutors: Dr. David Guerrero Setas, clinical biologist of Anatomic Pathology and principal investigator of the Molecular Pathology of Cancer Group and Iñaki Monreal Santesteban, laboratory technician. These practices were dual, had 2 different stays in the Molecular Pathology of Cancer Research Group of Navarrabiomed and the Anatomic Pathology Service of the CHN. Within the internship, besides learning routine work techniques, I was assigned to carry out a small project that consisted of optimizing and fine-tuning the conditions to detect and localize by immunohistochemistry the following markers in paraffin-embedded breast tissue: Claudin 2, carbonic anidrase 9, NMDAR2R, NOX4 and SLAMF9. This work will serve to complement a larger study that will soon be published in an article. The techniques I learned were: bisulfite treatment, pyrosequencing, Western blotting, maintenance of cell lines in in vitro cell cultures, immunohistochemistry, tissue miroarrays, extraction, amplification and quantification of nucleic acids, gene knock out, RT PCR and preparation of library pools for sequencing by Ion Torrent silicon semiconductor chips. Show less
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Complejo Hospitalario de Navarra
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Spain
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Hospitals and Health Care
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200 - 300 Employee
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Ad honorem internship at the Clinical Microbiology Service of the CHN (140 hours).
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Jul 2021 - Jul 2021
Tutor: Dr. María Eugenia Portillo Bordonabe. Assistant microbiologist at CHN and head of the antibiotic resistance laboratory. It allowed me to practically expand my knowledge of microbiology and to be helping in the diagnosis of SARS-CoV-2 during the fifth wave of the pandemic. I rotated through all sections of the Clinical Microbiology Service: - Specimen Receipt. - Urine, dermatophytes and STDs. - Parasitology and stool cultures. - Upper and lower respiratory. - Blood cultures, organic liquids and exudates. - Serology and viral loads - PCRs and antibiotic laboratories. Show less
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Clínica Universidad de Navarra
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Spain
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Health and Human Services
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700 & Above Employee
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Ad honorem graduate internship at the Anatomic Pathology Service of the CUN (115 hours).
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Jun 2021 - Jun 2021
Tutor: Dr. Lola Lozano Escario. Medical anatomopathologist and head of the Anatomic Pathology Service of the CUN. Tasks: - Assisting laboratory technicians in the processing of clinical samples. - Performing manual immunohistochemistry and immunohistochemistry with equipment. ISH, FISH and PCR. - Use of microtome and cryostat. - Histochemical staining. - Learning of macroscopic histology in the carving, analysis and description of organs together with medical specialists. I also attended curricular formative internships that third year medical students took in the summer. - Myroscopic analysis and diagnosis together with anatomopathologist specialists. - Organization, scanning and archiving of clinical preparations. - Assisted in performing monoplex and multiplex for immunopathology research studies along with image analysis with ImageJ. - Attendance at daily department meetings. Show less
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Cima Universidad de Navarra
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Spain
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Research Services
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100 - 200 Employee
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FDP: Histological characterization of radiotherapy-induced brain radionecrosis (12 ECTS)
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Jul 2020 - May 2021
Grade: 9.2 (Outstanding). Tutor: Dr. Diego Serrano Tejero. My FDP in Biology was experimental and I developed it in the Laboratory of Biomarkers and new therapeutic targets for lung cancer led by Dr. Luis Montuenga Badía and belonging to the Solid Tumors Program of CIMA. Techniques learned: immunohistochemistry, double and multiplex immnofluorescence, scanning and bioinformatics image analysis with ImageJ and Aperio and Nissls staining. Summary: Cerebral radionecroses are among the major adverse effects of stereotactic radiotherapy treatments employed to treat brain tumors. Their pathophysiology is thought to be caused by damage to endothelial and glia cells, leading to hemorrhage, hypoxia and brain necrosis accompanied by angiogenesis and inflammation. There are indications that the STAT3 protein regulates these processes and could therefore be an effective therapeutic target to combat radionecrosis. The objectives of the present work are a) To verify whether the drug Eurosil 85 is effective in reducing STAT3 (pSTAT3) phosphorylation levels in brain radionecrosis. b) To set up a multiplex system (using the Vectra Polaris system of Akoya Biosciences) to colocalize and quantify GFAP, F4/80 and pSTAT3 in radionecrosis. c) To histologically characterize radionecrosis lesions. Show less
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Junior Achievement España
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Spain
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Education
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1 - 100 Employee
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Business idea project
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Nov 2020 - Apr 2021
Startup Programme is an educational program, developed in 15 European countries in parallel, which aims to promote entrepreneurship in the university environment as well as to create a community of young entrepreneurs. A group of UNAV students formed a multidisciplinary team that carried out the development of a business idea about the implementation of an online marketplace focused on local organic products. We were awarded when we reached the semi-final stage and for me it was an enriching experience because of what I learned and the people I met, helping me to improve my analytical, leadership, teamwork and social skills. Show less
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University of Navarra
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Spain
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Education Management
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700 & Above Employee
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Practical work of the subject of Plant Biodiversity (3 ECTS)
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Sep 2020 - Dec 2020
Grade: 9 (Outstanding).Supervisor: Dr. Ricardo Ibañez Gastón.This work was developed over 4 months, it represented almost the entire grade of the subject of Plant Biodiversity and required a great effort to do it because it was totally practical and consisted of a taxonomic classification work on a group of plants. In my case I did it on angiosperms and I titled it: Angiosperms of the Pamplona basin and surroundings.I identified 66 different specimens: 50 at species level, 9 at genus level and 7 at family level, with a biodiversity of 29 different families. I gained skills in the use and management of dichotomous keys to identify plant species and reinforced my knowledge about the main characteristics of the large groups of angiosperms. 🌺🌼🌷🌻🥀🏵️💮🌸🌾☘️ Show less
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Ad honorem student intern of the AMBIUN Plant Stress Physiology Group (105 hours)
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Aug 2020 - Aug 2020
Tutor: Dr. Inmaculada Pascual Elizalde. Researcher at the AMBIUN of the UNAV.Tasks:- Collaboration in experiment on several grapevine varieties 🍇 (Cavernet Souvignon, Ambrosina, Garnacha, Tinto Velasco, Pasera, Merlot, Tempranillo 43 and Tempranillo 24) and their impact on climate change.- Collaboration in experiments to determine the transipration of a grapevine cluster.🍃- Collaboration in the preparation of the practices of the subject Plant Physiology. Learning of plant physiology techniques: stomata replication, handling of dualex for chlorophyll determination, extraction and quantification of chlorophylls, handling of sporometers for stomatal conductance measurements, handling of pressure chamber for water potential determination, handling of spectroradiometer, transpiration and bunch respiration measurement chamber, high performance liquid chromatography (HPLC) equipment.- Greenhouse maintenance and cleaning work. Show less
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Innovation Factory Unav
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Spain
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Professional Training and Coaching
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1 - 100 Employee
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Entrepreneurship project in the Fast Track Program of the Innovation Factory of the UNAV (3 ECTS)
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Oct 2019 - May 2020
- Recognized with the award for the best business idea in the biosanitary field.🎖😊 Innovation Factory is the innovation department of the UNAV and develops a competition called Fast Track in which multidisciplinary teams are formed among students to find an idea to improve the world and develop it into a project that can go to market. We received training and feedback from UNAV professors and our work was tutored by Dámaso Molero CEO of 3P Biopharmaceuticals. In the team I proposed the idea we worked on and I played the role of CEO. The business idea we developed consisted in the application of oral probiotics based on Streptococcus dentisani 🧫🔬 bacteria as a viable proposal to prevent and treat caries🦷. In the team we were students from different disciplines from science, to marketing, ADE or engineering so it was important to develop a good communication. We also collaborated with institutions such as SODENA or CEIN who advised us and with the pharmaceutical company AB-Biotics.💊 It helped me with organization and working under pressure since I had to combine it with the third year of my degree in Biology. Show less
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Volkswagen Navarra
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Motor Vehicle Manufacturing
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200 - 300 Employee
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Ad honorem internship student in the Environmental Area of Volkswagen Navarra (120 hours)
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Aug 2019 - Aug 2019
Tutor: Dr. Ana Isabel Moreno Twose. Head of the Environmental Area of Volkswagen Navarra. The Volkswagen🏭 Group, European leader in the automotive market 🚗 and the second largest manufacturer worldwide, In Pamplona Volkswagen is the most powerful company in Navarra. The main functions of the Volkswagen Environment Area were: reaching the expectations of the ISO standards for environmental impact assessment in the automobile production process: CO2, VOCs, energy, water, recycling and environmental training of operators in Volkswagen Academy. Development of the Think Blue. Factory strategy, with which it is proposed to reduce, in the period 2010-2025, by 45% five indicators: energy and water consumption, generation of non-recoverable waste and CO2 and solvent emissions. Tasks: - Computer processing of environmental data (Excel, Word and Power Point).📊💻 - On-site visit of the facilities. Visited the different production sections of the industrial park: logistics, body shop, paint shop, scrubber, engines, assembly, quality and final check.🚗🏭 - Information about Volkswagen's car production process.📋🗃️ - Awareness of industrial impact on environmental biology.🌍🏭 - Accompanying environmental personnel, audits, participation in meetings, courses and reviews. Show less
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Profesional independiente
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Pamplona/Iruña, Comunidad Foral de Navarra, España
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Private tutoring teacher
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Oct 2018 - Jun 2019
I spent 9 months tutoring ESO and Bachillerato students on subjects such as Natural Sciences, Chemistry, Biology, Physics and Mathematics.👨🏫➗✖️🧪🧬. This experience was an opportunity for me to develop my teaching and communication skills. I was able to develop a work experience while studying, which was challenging. I spent 9 months tutoring ESO and Bachillerato students on subjects such as Natural Sciences, Chemistry, Biology, Physics and Mathematics.👨🏫➗✖️🧪🧬. This experience was an opportunity for me to develop my teaching and communication skills. I was able to develop a work experience while studying, which was challenging.
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Education
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2021 - 2022
University of Navarra
Master in Biomedical Research, Regenerative Medicine and Advanced Therapies (60 ECTS) -
2017 - 2021
Universidad de Navarra
Degree in Biology (240 ECTS), Biology/Biological sciences, fundamental -
2019 - 2021
University of Navarra
Diploma in Clinical Biology (26 ECTS), Health Services/Health Sciences, General -
2019 - 2021
Universidad de Navarra
Student intern in Oncology, Department of Anatomic Pathology and Physiology (200 hours) -
2021 - 2021
Universidad Europea Miguel de Cervantes
Degree in Molecular Biology (250 hours) (10 ECTS), Molecular Biology -
2018 - 2019
Universidad de Navarra
VI Edition of the Diploma in Management and Business Creation in Science (68 hours)
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