Experience

EpiBiologics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Aug 2023 - Present

  San Mateo, California, United States

• Associate Scientist

  • Sep 2022 - Aug 2023

  San Mateo, California, United States



Soteria Biotherapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Associate 1

  • Mar 2021 - Aug 2022

  San Francisco, California, United States • Purified and QC both in vitro and in vivo scale of complexed bispecifics and antigens for different applications • Characterized and analyzed biophysical properties of bispecifics for candidate selection • Performed and developed developability assessment assays to evaluate final leads

• Research Associate

  • Feb 2020 - Mar 2021

  San Francisco, California, United States • Designed and cloned therapeutic antibodies with different modalities • Maintained and transfected Expi293F cells for routine protein expression • Purified proteins with affinity purification techniques or processed via a AKTA FPLC • Characterized and analyzed biophysical properties of therapeutic antibodies via DSF and UHPLC-SEC • Maintained and implemented methods for instruments like AKTA and Vanquish UHPLC • Executed or optimized other assays like western blot, cell… Show more • Designed and cloned therapeutic antibodies with different modalities • Maintained and transfected Expi293F cells for routine protein expression • Purified proteins with affinity purification techniques or processed via a AKTA FPLC • Characterized and analyzed biophysical properties of therapeutic antibodies via DSF and UHPLC-SEC • Maintained and implemented methods for instruments like AKTA and Vanquish UHPLC • Executed or optimized other assays like western blot, cell surface staining via FLOW, phage selection and ELISA •Analyze experimental data and report key findings to the team regularly



Surrozen

• United States
• Biotechnology
• 1 - 100 Employee

• Research Associate II

  • Jan 2020 - Feb 2020

  Produced recombinant DNA clones and plasmids for development candidates. • Selected antibody candidates through characterizing two hybridoma campaigns via ELISA to differentiate epitopes (ECD/CBD),species cross-reactivity, and via FACS to asses specificity and cell surface binding capability. • Performed cell-based in vitro functional assays for evaluating potency and specificity. • Established and optimized protocol for ex vivo quantification of biomarkers of interest in liver… Show more Produced recombinant DNA clones and plasmids for development candidates. • Selected antibody candidates through characterizing two hybridoma campaigns via ELISA to differentiate epitopes (ECD/CBD),species cross-reactivity, and via FACS to asses specificity and cell surface binding capability. • Performed cell-based in vitro functional assays for evaluating potency and specificity. • Established and optimized protocol for ex vivo quantification of biomarkers of interest in liver tissue via ELISA and western blot. • Executed explorative experiments to determine mechanism of action of the molecule via transfection, transduction, and labelling of molecules. • Prepared presentations, reports and summary, and protocols.

• Research Associate

  • May 2018 - Dec 2019



DiscoverX Corporation

• United Kingdom
• Retail Office Equipment

• Research Associate

  • Sep 2017 - May 2018

  South San Francisco, CA Cell Culture: • Supported in-house human primary cell banking including CASMC, HLFa, NHBE, SAE, HDFn, HEKn, RPTE, and HUVE. • Assisted in preparing cell suspension, plating and collecting cell supernatant for assays. • Validated multiple types of harvested human primary cells for assays. • Used EVOS Cell Imaging System to identify and record cell morphology and proliferation. High Throughput Screening: • Performed high throughput cell-based and soluble assays to measure… Show more Cell Culture: • Supported in-house human primary cell banking including CASMC, HLFa, NHBE, SAE, HDFn, HEKn, RPTE, and HUVE. • Assisted in preparing cell suspension, plating and collecting cell supernatant for assays. • Validated multiple types of harvested human primary cells for assays. • Used EVOS Cell Imaging System to identify and record cell morphology and proliferation. High Throughput Screening: • Performed high throughput cell-based and soluble assays to measure essential biomarkers for biological multiplexed activity profiling(BioMAP). • Excelled in multiple bio-assays: alamarBlue, capture/sandwich ELISA, direct/indirect ELISA, HTRF, MSD, and SRB. • Operated and maintained HTS laboratory equipment such as liquid handling system, plate readers, washers, and dispensers via liquid handling software and Green Button Go. • Documented assay data and detailed procedures/reagents via ELN. Show less



Abcam

• United Kingdom
• Biotechnology Research
• 700 & Above Employee

• Lab Support Technician

  • Aug 2017 - Sep 2017

  Burlingame, California • Assisted hybridoma production via labeling plates, plating fusion cells,feeding 2X HAT media to cells and maintaining hybridoma cells for customers. • Facilitated different types of ELISA via labeling plates, preparing buffers, coating antigens, transferring cell supernatant, washing plates and reading data. • Maintenance of biosafety cabinets, incubators, water bath, centrifuges, refrigerator, and freezers (-80 °C, -20 °C).