zhixin (Ben) Shi

Fungal molecular biologist at Oerth Bio
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Contact Information
us****@****om
(386) 825-5501
Location
Raleigh-Durham-Chapel Hill Area

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Fungal molecular biologist
      • May 2022 - Present

    • Germany
    • Chemical Manufacturing
    • 700 & Above Employee
    • Sr Scientist
      • Sep 2012 - May 2022

      • Led and coordinated project planning and implementation • Led and coordinated HTP system for GCE (gene control elements) evaluation and phytotoxicity screening of transgene, improve and optimized HTP system for high data quality. • Identification and evaluation of promoters in transgenic crop. • Modified large T-DNA base vector; improved cloning, T-DNA delivery efficiency; enhanced transgene expression and quality of transgenic event • Bacterial strain engineering to improve cloning efficiency and construct stability • Troubleshooting poor expression of transgene • GM-and non-GM herbicide resistance/tolerance • Plant microbiome Show less

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Principle Scientist
      • Jan 2011 - Apr 2012

      • Initiated and led a project to improve the shelf life of commercial mushroom; successful proof-of-concept • Led a research effort to develop site-specific integration • Led and coordinated hybrid promoter development to improve protein expression • Initiated and led a project to improve the shelf life of commercial mushroom; successful proof-of-concept • Led a research effort to develop site-specific integration • Led and coordinated hybrid promoter development to improve protein expression

    • Australia
    • Biotechnology Research
    • 1 - 100 Employee
    • Sr. Scientist
      • Jun 2007 - Jan 2011

      • Led team effort of analyzing and evaluating gene control elements for transgene expression • Designed and implemented various molecular analysis methods for Agaricus bisporus system • Assisted in initial Agarigen laboratory set-up • Led team effort of analyzing and evaluating gene control elements for transgene expression • Designed and implemented various molecular analysis methods for Agaricus bisporus system • Assisted in initial Agarigen laboratory set-up

    • Research Scientist
      • Sep 2006 - Apr 2007

      • In vitro, in vivo protein expression profiling of target gene • Improved the yield of in vitro RNA production by improving promoter • Troubleshooting enzymatic capping process for difficult targets to improve the translational efficiency of RNA molecules in transient assay • In vitro, in vivo protein expression profiling of target gene • Improved the yield of in vitro RNA production by improving promoter • Troubleshooting enzymatic capping process for difficult targets to improve the translational efficiency of RNA molecules in transient assay

    • United States
    • Sr. Scientist
      • Jan 2003 - Aug 2006

      • Developed SOPs for target specific aptamer development for polychlorinated biphenyl (PCB) and BSA, including selection, high throughput screening, identification, and detection • Led a team effort of developing aptamer and protein antibody assay, developed PCR methods for strand-specific amplification, random library amplification, and GC-rich template to achieve high PCR yield • Developed SOPs for target specific aptamer development for polychlorinated biphenyl (PCB) and BSA, including selection, high throughput screening, identification, and detection • Led a team effort of developing aptamer and protein antibody assay, developed PCR methods for strand-specific amplification, random library amplification, and GC-rich template to achieve high PCR yield

    • Switzerland
    • Farming
    • 700 & Above Employee
    • Research Scientist
      • Nov 1999 - Dec 2002

      • High throughput streamline to identify target genes in Botrytis for fungicide development • Enabling techniques for functional genomics to improve throughput • High throughput streamline to identify target genes in Botrytis for fungicide development • Enabling techniques for functional genomics to improve throughput

Education

  • Washington State University Graduate School
    Ph. D., Cell/Cellular and Molecular Biology
  • NANJING AGRICULTURAL UNIVERSITY
    Bachelor of Science - BS
  • NANJING AGRICULTURAL UNIVERSITY
    Bachelor's degree, Plant Protection and Integrated Pest Management

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