zhixin (Ben) Shi
Fungal molecular biologist at Oerth Bio- Claim this Profile
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Bio
Experience
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Oerth Bio
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United States
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Biotechnology Research
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1 - 100 Employee
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Fungal molecular biologist
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May 2022 - Present
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BASF
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Germany
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Chemical Manufacturing
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700 & Above Employee
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Sr Scientist
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Sep 2012 - May 2022
• Led and coordinated project planning and implementation • Led and coordinated HTP system for GCE (gene control elements) evaluation and phytotoxicity screening of transgene, improve and optimized HTP system for high data quality. • Identification and evaluation of promoters in transgenic crop. • Modified large T-DNA base vector; improved cloning, T-DNA delivery efficiency; enhanced transgene expression and quality of transgenic event • Bacterial strain engineering to improve cloning efficiency and construct stability • Troubleshooting poor expression of transgene • GM-and non-GM herbicide resistance/tolerance • Plant microbiome Show less
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Intrexon Corporation
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United States
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Biotechnology
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1 - 100 Employee
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Principle Scientist
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Jan 2011 - Apr 2012
• Initiated and led a project to improve the shelf life of commercial mushroom; successful proof-of-concept • Led a research effort to develop site-specific integration • Led and coordinated hybrid promoter development to improve protein expression • Initiated and led a project to improve the shelf life of commercial mushroom; successful proof-of-concept • Led a research effort to develop site-specific integration • Led and coordinated hybrid promoter development to improve protein expression
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AgriGen Biotech
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Australia
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Biotechnology Research
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1 - 100 Employee
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Sr. Scientist
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Jun 2007 - Jan 2011
• Led team effort of analyzing and evaluating gene control elements for transgene expression • Designed and implemented various molecular analysis methods for Agaricus bisporus system • Assisted in initial Agarigen laboratory set-up • Led team effort of analyzing and evaluating gene control elements for transgene expression • Designed and implemented various molecular analysis methods for Agaricus bisporus system • Assisted in initial Agarigen laboratory set-up
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Argos Therapeutics, Inc. (NASDAQ: ARGS)
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Durham, NC
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Research Scientist
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Sep 2006 - Apr 2007
• In vitro, in vivo protein expression profiling of target gene • Improved the yield of in vitro RNA production by improving promoter • Troubleshooting enzymatic capping process for difficult targets to improve the translational efficiency of RNA molecules in transient assay • In vitro, in vivo protein expression profiling of target gene • Improved the yield of in vitro RNA production by improving promoter • Troubleshooting enzymatic capping process for difficult targets to improve the translational efficiency of RNA molecules in transient assay
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Syntherica Corp.
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United States
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Sr. Scientist
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Jan 2003 - Aug 2006
• Developed SOPs for target specific aptamer development for polychlorinated biphenyl (PCB) and BSA, including selection, high throughput screening, identification, and detection • Led a team effort of developing aptamer and protein antibody assay, developed PCR methods for strand-specific amplification, random library amplification, and GC-rich template to achieve high PCR yield • Developed SOPs for target specific aptamer development for polychlorinated biphenyl (PCB) and BSA, including selection, high throughput screening, identification, and detection • Led a team effort of developing aptamer and protein antibody assay, developed PCR methods for strand-specific amplification, random library amplification, and GC-rich template to achieve high PCR yield
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Syngenta
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Switzerland
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Farming
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700 & Above Employee
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Research Scientist
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Nov 1999 - Dec 2002
• High throughput streamline to identify target genes in Botrytis for fungicide development • Enabling techniques for functional genomics to improve throughput • High throughput streamline to identify target genes in Botrytis for fungicide development • Enabling techniques for functional genomics to improve throughput
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Education
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Washington State University Graduate School
Ph. D., Cell/Cellular and Molecular Biology -
NANJING AGRICULTURAL UNIVERSITY
Bachelor of Science - BS -
NANJING AGRICULTURAL UNIVERSITY
Bachelor's degree, Plant Protection and Integrated Pest Management