Experience

Link Cell Therapies

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist

  • Jan 2023 - Present



Gladstone Institutes

• United States
• Research Services
• 300 - 400 Employee

• Postdoctoral Fellow

  • Feb 2020 - Jan 2023



UCSF Health

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Postdoctoral Fellow

  • 2018 - 2020

  Technical Skills and Competence: • In Vivo: Mouse handling; subcutaneous and orthotopic injections of cancer cells to mice • Cell biology: Isolation of lysosomes from subcut/ortho tumors • Immunohistochemistry • Biochemistry: Immunoprecipitation-Mass Spec, Close proximity Labeling Assays (Turboid, BioID), Post Translational Modification analysis- Mass Spec • Cell Culture: Generation of stable cell lines- virus production and infection to the cells • Molecular Biology: Site-directed mutagenesis, overlap extension PCR, Isolation and analysis of DNA from cells, designing of primers and probes, molecular cloning, plasmids engineering, DNA sequence analysis, knock-down/overexpression of genes



The University of Freiburg

• Germany
• Research Services
• 700 & Above Employee

• PhD

  • 2011 - Nov 2016

  Main research project: Dissecting the regulation of the pro-apoptotic Bcl-2 protein Bax in mammalian cells. Technical Skills and Competence: • Cell Biology: Extensive cell culture experience in different cell lines and primary cell lines, chemical/liposome based transfections, • Cell Based Assays: Bacterial transformations, apoptotic assays-measurement of caspase3/7 activity, cytochrome c release assays, clonogenic cell survival assay • Molecular Biology: Site-directed mutagenesis, overlap extension PCR, Isolation and analysis of DNA from cells, designing of primers and probes, molecular cloning, plasmids engineering, DNA sequence analysis • Biochemistry Analysis: SDS-PAGE, Western Blot, Immunoprecipitation, Immunocytochemistry, whole cell lysate extraction, preparation of mitochondrial fraction from cells/tissue, ubiquitination assay, separation of soluble mitochondrial proteins from insoluble proteins, Native-page, BirA fusion assay, separation of phosphorylated proteins (Phos-tag gels) • Protein Biochemistry: 3D structure analysis and structure based protein manipulations • Imaging: Fluorescence Microscopy



İzmir Institute of Technology

• Research Services
• 200 - 300 Employee

• Research Assistant

  • Sep 2008 - Jan 2011

  Main research project: Investigating the involvement of ceramides and their metabolic products in drug induced cell death and resistance in leukemia cell lines • Identified therapeutic potential of adipose-derived mesenchymal stromal cells in the treatment of bone injuries. • Additional collaborative projects: Understanding the roles of STAT5A and topoisomerases in the chemotherapeutic sensitive/resistant chronic myeloid leukemia Technical Skills and Competence: • Cell Biology: Isolation of mesenchymal stromal cells from the adipose tissue and differentiation into the osteoblasts, cell culture experience in different leukemia cell lines and leukemia cells derived from patients, chemical/liposome based transfections, generation of modified cell lines with shRNAs/siRNAs, • Cell Based Assays: 96-well format MTT assay to measure cell proliferation and IC50 of drugs- analysis of sensitivity/resistance to the drugs, apoptotic assays-measurement of caspase3/7 activity and mitochondrial membrane potential • Molecular Biology: Isolation and analysis of DNA and RNA from cells, design of primers and probes, PCR, qPCR, molecular cloning, DNA sequence analysis, • Biochemistry Analysis: SDS-PAGE, Western Blot