Senior Specialist - Production

• Jul 2022 - Present

•Commercial Production of r-FSH using mammalian cell culture (Upstream Processing). •Optimization of depth filter area during clarification step. •Working towards the establishment of a new facility (XPandC) for MiGenTra. •Qualification of the wave bioreactors and the XDRs (200L and 1000L). •Drafted the batch record for a new product (Adalimumab) in the XPandC Facility. •Designed the setpoint tables (feeding and antifoam addition) and alarms for the proper functioning of the XDR Bioreactors. •Successfully executed the Engineering Run, and Technical Transfer Run for the Adalimumab project. Optimization of the process specifications as per the generated data. •Working towards development of an electronic batch record for the Adalimumab project. •Working towards GMP certification (and further certification from EMA) for the XPandC project. •Working on the transfer of a second project (Bevacizumab) in the XPandC facility. Show less

Production Officer

• Jul 2020 - Apr 2022

• Commercial Production (Upstream Processing) of Monoclonal antibodies (rituximab, bevacizumab, trastuzumab, tocilizumab and adalimumab). • Worked on the process development for the commercial production of the COVID Vaccine (Sputnik V). • Worked with the drafting the batch record for Tocilizumab project. • Worked on ramping up of the production scale (from 1kL to 2kL). • Drafted equipment SOPs for 200L and 2000L STR. • Worked on the Engineering Run and Process Validation Run for the Tocilizumab project. • Worked on execution of Investigations, Change Control and Risk Assessment Reports. Show less

Social Media Ambassador

• Dec 2019 - May 2020

Graduate Research

• Apr 2018 - Aug 2018

An investigative study on the modulation of vascular tone in mouse aortic vessels (Master’s Thesis). • C57BL/6 mice were dissected to isolate the aortic vessel. • The vessels were then prepared and mounted on the wire myograph. • Dosage Response Curves were generated in response to agonists (and blocked by antagonists) of the Sphingolipid pathway, to study the modulation of the vascular tone in the aorta. An investigative study on the modulation of vascular tone in mouse aortic vessels (Master’s Thesis). • C57BL/6 mice were dissected to isolate the aortic vessel. • The vessels were then prepared and mounted on the wire myograph. • Dosage Response Curves were generated in response to agonists (and blocked by antagonists) of the Sphingolipid pathway, to study the modulation of the vascular tone in the aorta.

Undergraduate Research

• Jan 2016 - May 2017

Analyzing the anti-angiogenic properties of peptides isolated from shark muscle and visceral mass hydrolysates. (Undergraduate Thesis) • Protein hydrolysates were isolated through enzymatic treatment. • Anti-oxidant (DPPH) and anti-inflammatory (albumin denaturation) assays were used to assess the bioactivity of the hydrolysate samples (due to the underlying connection between oxidation, inflammation and angiogenesis pathways). • The hydrolysates samples with the highest bioactivity were fractionated through Ultrafiltration (3 and 10 kDa filters), the bioactivity of each fraction was then assessed through anti-oxidant and anti-inflammatory assays. • The fractions with the highest bioactivity were purified through Gel Filtration Chromatography (Sephadex G-25). • The anti-angiogenic properties of the purified peptides were assessed through the Chick Chorio-Allantoic Membrane Assay (CAM’s were pre-treated with VEGF). The peptide dosage to be administered to the CAM’s was determined using MTT Assay (MCF-7) Analyzing the anti-oxidant properties of peptides isolated from tuna muscle hydrolysates. • Protein hydrolysates were isolated through enzymatic treatment. • Anti-oxidant assays (DPPH and Reducing Power Assay) were used to assess the anti-oxidant properties of the protein hydrolysates. • Hydrolysate samples showing the highest anti-oxidant properties were fractionated using Ultrafiltration (3 and 10 kDa filters). • Anti-oxidant assays (DPPH and Reducing Power Assay) were used to assess the fraction with the highest anti-oxidant properties. Show less