Experience

LinXis Biopharmaceuticals

• Netherlands
• Biotechnology Research
• 1 - 100 Employee

• Head of Pharmaceutical Development

  • Jan 2023 - Present



Kiadis Pharma

• Netherlands
• Biotechnology
• 1 - 100 Employee

• Director Analytical development

  • Mar 2021 - Dec 2022

• Principal Scientist Analytical Development

  • Jan 2020 - Feb 2021

• Senior Scientist Analytical Development and Quality Control

  • May 2018 - Dec 2019



uniQure

• Netherlands
• Biotechnology Research
• 400 - 500 Employee

• Senior Scientist Analytical Development/Team Lead Product Support

  • Jan 2014 - Apr 2018

  o Supervision Scientists and Technicians Analytical Development, Product support groupo Method development, qualification and reporting of analytical packages supporting AAV-based product release testing o Reference standard qualificationo Master and Working Cell bank and Viral Seeds qualificationo Phase I/II product release and stability testing, support QP releaseo Setting product specificationso Extended product characterization (Phase I/II, Phase III and commercial products)o Product comparability (Phase I/II, Phase III and commercial products)o Assay validation according ICH Q2o Assay trouble shooting o Contribution to Module 3 of IND/IMPD and MAA dossierso Assay transfer to Quality Control department uniQure Lexington facilityo AD Lead Scientist in multi-disciplinary CMC project teams

• Scientist Analytical Development

  • Nov 2010 - Dec 2013

  o Responsible for the development of molecular biological assays (e.g. qPCR, PCR and gel based assays) o Setup assay validation strategies for Q-PCR methods according to ICH guidelineo Manage development of molecular biological assays at CRO o Additional (non-routine) characterization of rAAV products o Contribution to registration dossier (CMC section)o Manage assay transfer activities to Quality Control departmento Reference standards

• Scientist Vector Development

  • Mar 2007 - Nov 2010

  o Main focus to improve baculovirus-based rAAV production process using directed protein evolution and molecular biological approacheso rAAV product characterization using molecular biological and biochemical methodso Contribution to registration dossier (CMC section)



Radboudumc

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Ph D student

  • Oct 2002 - Oct 2006

  o Subject: Protein tyrosine phosphatase PTPRR isoforms function as multimeric brain-specific MAPK phosphatases o Techniques: Tissue culture, transfections, retroviral transduction, pull-down assays, co-immunoprecipitations, SDS-PAGE, quantitative Western blotting (Odyssey infrared imaging system), RT-PCR, PCR, cloning, site directed mutagenesis, RNA interference, immunohistochemistry, immunofluorescence and confocal laser scanning microscopy. o Subject: Protein tyrosine phosphatase PTPRR isoforms function as multimeric brain-specific MAPK phosphatases o Techniques: Tissue culture, transfections, retroviral transduction, pull-down assays, co-immunoprecipitations, SDS-PAGE, quantitative Western blotting (Odyssey infrared imaging system), RT-PCR, PCR, cloning, site directed mutagenesis, RNA interference, immunohistochemistry, immunofluorescence and confocal laser scanning microscopy.