Experience

Kernal Biologics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Scientist

  • Oct 2022 - Present



SiPhox Inc.

• United States
• Semiconductor Manufacturing
• 1 - 100 Employee

• Research Scientist

  • Jan 2021 - Sep 2022

  • Developed 5 immunoassays (direct, sandwich, competitive) and generated a plan for assay submission for FDA approval • Performed assay design and development, ELISA, GatorBio, antibody conjugation to nanoparticles, IgG purification, antibody screening • Built and managed a BSL2 lab • Worked with external vendors • Hired technical assistants • Developed 5 immunoassays (direct, sandwich, competitive) and generated a plan for assay submission for FDA approval • Performed assay design and development, ELISA, GatorBio, antibody conjugation to nanoparticles, IgG purification, antibody screening • Built and managed a BSL2 lab • Worked with external vendors • Hired technical assistants



ATLAS ONCOLOGY LLC

• United States
• 1 - 100 Employee

• Research Scientist

  • Aug 2019 - Oct 2020

  • Analyzed tumor genomic profiles of 100 patients to identify biomarkers of targeted cancer therapies and prepared reports describing efficient personalized therapeutic approaches • Selected companion diagnostics tests for molecular profiling of tumor samples • Managed a team of 3 people to build a database of predictive tumor biomarkers • Analyzed tumor genomic profiles of 100 patients to identify biomarkers of targeted cancer therapies and prepared reports describing efficient personalized therapeutic approaches • Selected companion diagnostics tests for molecular profiling of tumor samples • Managed a team of 3 people to build a database of predictive tumor biomarkers



Massachusetts Institute of Technology

• United States
• Higher Education
• 700 & Above Employee

• Postdoctoral Scientist

  • Sep 2014 - Jun 2019

  Supervisor: Daniel Griffith Anderson, PhD • Developed an IVT-mRNA system for in vivo delivery of the humanized anti-HER2 antibody trastuzumab and demonstrated its anticancer activity in vitro using human breast cancer cell lines, ex vivo using an ADCC assay, and in vivo using a mouse xenograft model. • Developed a protocol for Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) assay. • Engineered a breast cancer mouse model for analysis of trastuzumab mRNA efficacy. • Performed high-throughput loss-of-function shRNA-based screening. Identified a new lncRNAs (ASTILCS) essential for survival of human liver cancer cells and characterized its molecular mechanism. • Collaborated with the Robert Langer lab, designed and executed in vitro and in vivo experiments to test anticancer activity of novel drug-excipient combinations predicted by a computer-aided drug design. • Performed molecular cloning (Golden Gate, Gibson assembly, Gateway, traditional), cell culture (primary cells and established cell lines), flow cytometry (TUNEL assay, BrdU and Click-iT EdU cell cycle analysis), Western blotting, qPCR, IgG purification, IVT-mRNA synthesis, ELISA, high-throughput genetic screening (RNAi), lentiviral transduction, gene knockdown (using RNAi, CRISPRi, antisense oligonucleotides), cell transformation (Lipofectamine, lentiviral), cell-based assays (survival, proliferation), PCR primer design, sgRNA/shRNA design. Proficient in using SnapGene, ImageJ, FlowJo, ModFit, GraphPad Prism. • Worked with mice: hydrodynamic and tail vein injections, tail vein and submandibular bleeding, generation of subcutaneous xenografts, generation of liver cancer genetic model, survival experiments, dissection • Hired, mentored, and trained technicians, students, and postdocs Show less



The Research Center of Neurology

• Moscow, Russia

• Junior Researcher

  • Jun 2009 - Mar 2014

  Worked on PhD project: • Studied the effects of natural antioxidant carnosine and its derivatives on cell cycle progression in pheochromocytoma cells (PC-12). Performed structure–function analysis to investigate the mechanism of carnosine antiproliferative effect. • Characterized carnosine’s protective effects on cerebellar neurons oxidative damage. • In collaboration with St.-Petersburg Institute of Bioregulation and Gerontology described the effects of synthetic antioxidant pinealon on cancer cell survival and cell cycle progression. • In collaboration with the Department of Biochemistry of Russian Peoples' Friendship University in Moscow investigated molecular mechanism of antitumor effect of L-Amino acid oxidases. • Performed: flow cytometry (cell cycle analysis with PI, ROS analysis with DCFH-DA), cell culture (PC-12), immunoblotting, qRT-PCR. Show less



University of Iowa Health Care

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Graduate Research Assistant

  • Aug 2012 - Jun 2013

  Worked on PhD project: • Characterized the mechanism of glioblastoma cell growth inhibition by short-chain neuropeptide carnosine and its derivatives. Extensively analyzed cell cycle. • Demonstrated radiosensitizing capacity of plant alkaloid piperlongumine in head and neck cancer cells. • Performed: flow cytometry (DNA content analysis with PI, BrdU cell cycle analysis, ROS analysis with DCFH-DA), cell culture (glioblastoma, breast and head and neck cancer cells), cell proliferation assay, clonogenic survival assay, enzyme activity gel (for MnSOD), immunoblotting, qRT-PCR. Show less



Summer scientific adventure children program “Vsemu uchit more”

• Sukko, Krasnodarskiy kray, Russia

• Biology Tutor

  • Jul 2008 - Jul 2009

  Taught biology and ecology and organized scientific entertainment events Taught biology and ecology and organized scientific entertainment events



A.N. Belozersky Institute of Physico-Chemical Biology of Lomonosov Moscow State University

• Moscow, Russia

• Undegraduate Research Assistant

  • Sep 2007 - Jun 2009

  Worked on Specialist's research project: • Identified and characterized dedifferentiation effects of synthetic mitochondria-targeted antioxidants on neuroblastoma and glioblastoma cells. Extensively analyzed cytoskeleton rearrangements • Performed cell culture (neuroblastoma and glioblastoma cell lines), immunocytochemistry, fluorescence microscopy, immunoblotting, qRT-PCR • Analyzed and interpreted data, presented at international conferences Worked on Specialist's research project: • Identified and characterized dedifferentiation effects of synthetic mitochondria-targeted antioxidants on neuroblastoma and glioblastoma cells. Extensively analyzed cytoskeleton rearrangements • Performed cell culture (neuroblastoma and glioblastoma cell lines), immunocytochemistry, fluorescence microscopy, immunoblotting, qRT-PCR • Analyzed and interpreted data, presented at international conferences