Yubing Li

Senior Scientist at FfAME - Foundation for Applied Molecular Evolution
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(386) 825-5501
Location
Gainesville, Florida, United States, US
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Credentials

  • Intro to Python for Data Science Course
    DataCamp
    Aug, 2018
    - Oct, 2024

Experience

  • FfAME - Foundation for Applied Molecular Evolution
    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Senior Scientist
      • Oct 2018 - Present

      Origin of life Artificial life with expanded genetic alphabets Cell engineering Endosymbiosis and evolution, Synthetic biology Origin of life Artificial life with expanded genetic alphabets Cell engineering Endosymbiosis and evolution, Synthetic biology

  • University of Florida
    • United States
    • Higher Education
    • 700 & Above Employee
    • Senior Research Biologist
      • 2013 - 2018

      Research project leader Supervise undergraduate student and graduate students Manage lab operation Projects: •  Investigated phenylpropanoid biosynthetic pathways (including plastoquinone, phylloquinone, vitamin E, and ubiquinone) in plants, identified and characterized novel enzymes, and further engineered those pathways/enzymes in bacterial, yeast or plants through synthetic biology. • Cloned and characterized plant ubiquinone biosynthesis genes and expressed in E. coli… Show more Research project leader Supervise undergraduate student and graduate students Manage lab operation Projects: •  Investigated phenylpropanoid biosynthetic pathways (including plastoquinone, phylloquinone, vitamin E, and ubiquinone) in plants, identified and characterized novel enzymes, and further engineered those pathways/enzymes in bacterial, yeast or plants through synthetic biology. • Cloned and characterized plant ubiquinone biosynthesis genes and expressed in E. coli mutants and analyzed their enzyme activities/substrate specificities using HPLC based analytical assays. • Engineered Vitamin E biosynthetic pathways in E. coli to produce un-natural phenylpropanoid products. • Identified novel protein-protein interactions among plastoglobins, phenylpropanoid biosynthetic enzymes in Arabidopsis using both GAL4 and split-ubiquitin based yeast two-hybrid systems.  Characterized a 3‐methylglutaconyl‐CoA hydratase in branched-chain amino acid degradation pathways in plants and studied its potential application in crop nutrient improvement.  Studied transporters and membrane protein trafficking, assembly, structure and functions in plant chloroplasts. • Characterized a novel membrane translocase complex -Sec2 and its protein substrates in plant chloroplasts and investigated their functions in plant chloroplast biogenesis, photosynthesis, and plant development using inducible RNAi, yeast two-hybrid screening, transient expression in protoplasts, and chloroplast in vitro import/integration approaches. • Using inducible RNAi systems to generate Sec2, Sec1 knock-down mutant lines in Arabidopsis; characterized mutant phenotypes and analyzed chloroplast gene expression and protein abundance in those mutants. • Developed new in-vitro organelle-free assays for analyzing protein reconstitution into chloroplast membranes. • Characterized a SecY2 Mu insertion mutant in maize. Show less Research project leader Supervise undergraduate student and graduate students Manage lab operation Projects: •  Investigated phenylpropanoid biosynthetic pathways (including plastoquinone, phylloquinone, vitamin E, and ubiquinone) in plants, identified and characterized novel enzymes, and further engineered those pathways/enzymes in bacterial, yeast or plants through synthetic biology. • Cloned and characterized plant ubiquinone biosynthesis genes and expressed in E. coli… Show more Research project leader Supervise undergraduate student and graduate students Manage lab operation Projects: •  Investigated phenylpropanoid biosynthetic pathways (including plastoquinone, phylloquinone, vitamin E, and ubiquinone) in plants, identified and characterized novel enzymes, and further engineered those pathways/enzymes in bacterial, yeast or plants through synthetic biology. • Cloned and characterized plant ubiquinone biosynthesis genes and expressed in E. coli mutants and analyzed their enzyme activities/substrate specificities using HPLC based analytical assays. • Engineered Vitamin E biosynthetic pathways in E. coli to produce un-natural phenylpropanoid products. • Identified novel protein-protein interactions among plastoglobins, phenylpropanoid biosynthetic enzymes in Arabidopsis using both GAL4 and split-ubiquitin based yeast two-hybrid systems.  Characterized a 3‐methylglutaconyl‐CoA hydratase in branched-chain amino acid degradation pathways in plants and studied its potential application in crop nutrient improvement.  Studied transporters and membrane protein trafficking, assembly, structure and functions in plant chloroplasts. • Characterized a novel membrane translocase complex -Sec2 and its protein substrates in plant chloroplasts and investigated their functions in plant chloroplast biogenesis, photosynthesis, and plant development using inducible RNAi, yeast two-hybrid screening, transient expression in protoplasts, and chloroplast in vitro import/integration approaches. • Using inducible RNAi systems to generate Sec2, Sec1 knock-down mutant lines in Arabidopsis; characterized mutant phenotypes and analyzed chloroplast gene expression and protein abundance in those mutants. • Developed new in-vitro organelle-free assays for analyzing protein reconstitution into chloroplast membranes. • Characterized a SecY2 Mu insertion mutant in maize. Show less

  • Salk Institute for Biological Studies
    • United States
    • Research Services
    • 700 & Above Employee
    • Research Associate
      • 2006 - 2011

      Projects: • Generated and screened cell size/cell cycle mutant library in green alga, Chlamydomonas reinhardtii. • Characterized a novel cyclin dependent kinase, CDKG1, in Chlamydomonas and investigated its function in cell cycle/cell size and cell growth regulation. • Optimized culture conditions for algal mutant and wild type strains and developed new assay methods for cell progression. • Studied the function of cell cycle genes in flagella/cilia length control in green algae and… Show more Projects: • Generated and screened cell size/cell cycle mutant library in green alga, Chlamydomonas reinhardtii. • Characterized a novel cyclin dependent kinase, CDKG1, in Chlamydomonas and investigated its function in cell cycle/cell size and cell growth regulation. • Optimized culture conditions for algal mutant and wild type strains and developed new assay methods for cell progression. • Studied the function of cell cycle genes in flagella/cilia length control in green algae and mammalian cells. • Provided novel evidence on the effect of cilia length control on cell cycle regulation, metabolism and cancer inhibition. Show less Projects: • Generated and screened cell size/cell cycle mutant library in green alga, Chlamydomonas reinhardtii. • Characterized a novel cyclin dependent kinase, CDKG1, in Chlamydomonas and investigated its function in cell cycle/cell size and cell growth regulation. • Optimized culture conditions for algal mutant and wild type strains and developed new assay methods for cell progression. • Studied the function of cell cycle genes in flagella/cilia length control in green algae and… Show more Projects: • Generated and screened cell size/cell cycle mutant library in green alga, Chlamydomonas reinhardtii. • Characterized a novel cyclin dependent kinase, CDKG1, in Chlamydomonas and investigated its function in cell cycle/cell size and cell growth regulation. • Optimized culture conditions for algal mutant and wild type strains and developed new assay methods for cell progression. • Studied the function of cell cycle genes in flagella/cilia length control in green algae and mammalian cells. • Provided novel evidence on the effect of cilia length control on cell cycle regulation, metabolism and cancer inhibition. Show less

  • UC Riverside
    • Riverside, CA
    • Postgraduate Researcher
      • 2004 - 2006

      Projects: • Studied metabolic function and subcellular localization of lysophosphatidyl acyltransferase, LPAT2, in Arabidopsis and its role in plant lipid biosynthesis. • Purified and characterized major pollen coat proteins and flavonoids from maize. • Investigated the storage and delivery modes of pollen coat proteins and flavonoids in maize tapetum cells. Projects: • Studied metabolic function and subcellular localization of lysophosphatidyl acyltransferase, LPAT2, in Arabidopsis and its role in plant lipid biosynthesis. • Purified and characterized major pollen coat proteins and flavonoids from maize. • Investigated the storage and delivery modes of pollen coat proteins and flavonoids in maize tapetum cells.

Education

  • The Chinese University of Hong Kong
    Ph.D, Plant Cell and Molecular Biology
    2000 - 2004
  • Nankai University
    Master, Microbiology
    1993 - 2000

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