Experience

Star Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Principal Scientist

  • Mar 2023 - Present

  South San Francisco, California, United States

• Senior Scientist

  • Sep 2020 - Feb 2023

  South San Francisco, California, United States



Bio-Rad Laboratories

• United States
• Biotechnology Research
• 700 & Above Employee

• Senior Scientist

  • Sep 2015 - Aug 2020

  Hercules, CA USA - Troubleshooting technical complaints, identifying root cause, and generating technically sound corrective actions for Bio-Rad's HPLC hemoglobin A1c and hemoglobinopathy testing platforms (CAPA). - Test design per CLSI guidelines, execution, data analysis and report writing. - Development of Latex-based Immunoassay (POC; Point-of-Care diagnostics). Reagent development by antibody-solid phase conjugation. - Method & product evaluation of various POC diagnostics technologies and… Show more - Troubleshooting technical complaints, identifying root cause, and generating technically sound corrective actions for Bio-Rad's HPLC hemoglobin A1c and hemoglobinopathy testing platforms (CAPA). - Test design per CLSI guidelines, execution, data analysis and report writing. - Development of Latex-based Immunoassay (POC; Point-of-Care diagnostics). Reagent development by antibody-solid phase conjugation. - Method & product evaluation of various POC diagnostics technologies and products (accuracy, precision, interference). - Research for next generation diabetes biomarkers - Optimization of cell-free DNA extraction for a ddPCR-based diagnostic test development. - Analytical method development, qualification, validation, training scientists and resolution of technical issues. - Reviewing technical documents, reports, and protocols to ensure accuracy and scientific merit. - Maintaining complete and accurate GMP compliant documentation of experiments. - Stability test of reagents and investigation of OOS/OOT products in conjunction with QC group Quality control). - Instrument/equipment qualification (IQ, OQ, PQ) Show less



Diadexus

• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Feb 2013 - Aug 2015

  349 Oyster Point Blvd, South San Francisco, CA. - Hybridoma cell culture and purification of monoclonal antibodies for Lp-PLA2 product using Protein A affinity, Cation exchange and Anion exchange chromatographies. Optimization of HRP conjugation for the detector antibody. - Made key contributions to the development of two successful prototype EIA assays for MR-proADM and MR-proANP (heartfailure biomarkers). - Lead the development program and antibody configuration study for the CT-proET-1 ELISA assay. - Lead research programs and… Show more - Hybridoma cell culture and purification of monoclonal antibodies for Lp-PLA2 product using Protein A affinity, Cation exchange and Anion exchange chromatographies. Optimization of HRP conjugation for the detector antibody. - Made key contributions to the development of two successful prototype EIA assays for MR-proADM and MR-proANP (heartfailure biomarkers). - Lead the development program and antibody configuration study for the CT-proET-1 ELISA assay. - Lead research programs and refined the designs of reagents in order to provide experimental data to support the submission of three provisional patents and one in preparation. - Performed comparison studies of different StreptAvidin-coated ELISA plates. - Developed and supplied reagents and materials for development of assay to measure MR-proANP, MR-proADM, CT-proET-1, three heartfailure biomarkers. - Developed alternative reagent configurations to support the team goal of having alternate development strategies available if needed. Various strategies such as primary amine-, carboxyl-, sugar-targeted biotinylation and Digoxigenin chemistry have been employed. - Managed multiple production and protein/Antibody engineering projects that involved various CROs. - Supervised the transfer of the licensed materials between diaDexus and a collaboration partner. - Supervised the collection of human plasma and serum samples used for assay development. - Supervised the installation, validation, maintenance of a BRAMS KRYPTOR compact analyzer system. - Participated in feasibility studies for the next generation assays (diaDexus SPEAR process). - Produced SNPs (single nucleotide polymorphismic variants) of Lp-PLA2 to study their physiochemical properties. - Developed a LC/SEC based assay to study interaction between lipoprotein (LDL/HDL) and Lp-PLA2 variants. - Demonstrated that Lp-PLA2 SNPs have different enzymatic activities and reactivity towards antibodies. Show less



Catalyst Biosciences

• United States
• Biotechnology Research
• 1 - 100 Employee

• Staff Scientist

  • Jul 2005 - Feb 2013

  260 Littlefield Ave. South San Francisco, CA. Protein expression/purification from E. coli, Pichia, mammalian cell culture (CHO, BHK, HEK 293). Protein solubilization, Refolding, Optimization of refolding, Chromatographies (IEX, SEC, HIC, Affinity(IMAC, FLAG, HisTag, Heparin etc.)), TFF, Protein characterization, Protein modification (PEGlyation, Fc-Fusion, Biotinylation etc), assay development, analytical HPLC (RP, SEC, IEX etc. Agilent, Shimadzu), Automated purification using AKTA/FPLC, AKTA Pilot systems, Protein biochemstry, Molecular… Show more Protein expression/purification from E. coli, Pichia, mammalian cell culture (CHO, BHK, HEK 293). Protein solubilization, Refolding, Optimization of refolding, Chromatographies (IEX, SEC, HIC, Affinity(IMAC, FLAG, HisTag, Heparin etc.)), TFF, Protein characterization, Protein modification (PEGlyation, Fc-Fusion, Biotinylation etc), assay development, analytical HPLC (RP, SEC, IEX etc. Agilent, Shimadzu), Automated purification using AKTA/FPLC, AKTA Pilot systems, Protein biochemstry, Molecular biology, Bacterial fermentation (Fed Batch, Batch mode). Show less



UCSF

• Postdoc, Postgraduate Researcher

  • Jan 1999 - Jun 2005

  San Francisco Bay Area Protease Proteinase Peptidase substrate specificity, Positional scanning synthetic combinatorial substrate libraries, Protease inhibitors, Protein overexpression using microorganisms, E. coli, Pichia, mammalian cell culture, Protein solubilization & refolding, Chromatographies, Purification, Characterization, assay development, HPLC (preparative & analytical), Organic chemistry, Biochemstry, Molecular biology, Cloning, Malaria-related proteases and inhibitors as Drug development candidates… Show more Protease Proteinase Peptidase substrate specificity, Positional scanning synthetic combinatorial substrate libraries, Protease inhibitors, Protein overexpression using microorganisms, E. coli, Pichia, mammalian cell culture, Protein solubilization & refolding, Chromatographies, Purification, Characterization, assay development, HPLC (preparative & analytical), Organic chemistry, Biochemstry, Molecular biology, Cloning, Malaria-related proteases and inhibitors as Drug development candidates, Project management. Show less



Myongji University

• Lecturer, Postdoc

  • Dec 1997 - Nov 1998

  Korea Taught college students (Biology), Supervised graduate students (Purification, Chromatographies, Biochemistry, Biotechnology, Fermentation etc.)