Experience

Northwest A&F University

• China
• Higher Education
• 1 - 100 Employee

• Professor

  • Jan 2014 - Present

  Yangling, Shaanxi Province, China



Duke University

• United States
• Higher Education
• 700 & Above Employee

• Research Associate

  • Nov 2010 - Dec 2013

  LC/MS/MS; 2D-LC/HDMSE; bacteria; Ant symbiont; Gut microorganisms



Syngenta

• Switzerland
• Farming
• 700 & Above Employee

• Technical Expert II

  • Aug 2009 - Oct 2010

  • Evaluated the potential synergism and antagonism of Bt proteins with respect to insecticidal activity using sensitive insect species such as European corn borer, Fall armyworm, Western corn rootworm, and Colorado potato beetle • Extracted and partially purified proteins from corn and soybean with immunoaffinity columns and other filtration devices • Compared the biochemical and functional activities of protein of interest from both plant and microbe sources via immunological methods… Show more • Evaluated the potential synergism and antagonism of Bt proteins with respect to insecticidal activity using sensitive insect species such as European corn borer, Fall armyworm, Western corn rootworm, and Colorado potato beetle • Extracted and partially purified proteins from corn and soybean with immunoaffinity columns and other filtration devices • Compared the biochemical and functional activities of protein of interest from both plant and microbe sources via immunological methods such as ELISA • Prepared SDS-PAGE and Western blot for peptide mapping and N-terminal amino acid sequence analysis • Created and reviewed SOPs, assay development reports, and study reports Show less



KBI Biopharma

• United States
• Biotechnology Research
• 700 & Above Employee

• Formulation Scientist

  • May 2009 - Aug 2009

  • Studied conformational and biological activity of therapeutic proteins via Circular Dichroism for secondary structure change, Capillary Electrophoresis for glycans detection and identification, Dynamic Light Scattering for protein congregation etc. • Monitored the stability of antibody drugs with HPLC, A280, CD, DLS, pH, color etc. • Studied the different formulations on the stability of antibody drugs



Biolex

• Biotechnology
• 1 - 100 Employee

• Research Scientist

  • Oct 2008 - May 2009

  • Research scientist in Protein Analytical Development Group • Developed an ELISA assay for quantifying a protein of interest • Developed and qualified an assay to study the in vitro release kinetics of the encapsulated proteins from microspheres (Controlled Drug Release) via HPLC • Developed a liquid media turbidity measurement assay



NC State University

• Research Specialist

  • Feb 2005 - Oct 2008

  • Studied the mechanisms by which hormones cause changes in behavior and physiology in honey bees and wasps by using DNA microarray, qRT-PCR, in situ hybridization etc. techniques • Investigated the proteins relating to honey bee foraging behavior by using BCA protein assay, SDS-PAGE, Western Blotting, immunoprecipitation, immunohistochemistry etc. • Managed the lab and helped graduate students on their research projects and provided technical expertise



The University of Georgia

• Higher Education
• 700 & Above Employee

• Post-doctoral Research Associate

  • Jul 2004 - Feb 2005

  •Isolated and purified several insulin-like peptides from the yellow fever mosquito Aedes aegypti and the African malaria mosquito, Anopheles gambiae •Molecularly cloned and sequenced 8 insulin-like peptide genes and elucidated their tissue-, stage- and sex-specific expressions in the yellow fever mosquito, Aedes aegypti •Explored the interactions between insulin-like peptides and their receptor/s in two mosquito species •Developted and validated ELISA for measuring the insulin-like… Show more •Isolated and purified several insulin-like peptides from the yellow fever mosquito Aedes aegypti and the African malaria mosquito, Anopheles gambiae •Molecularly cloned and sequenced 8 insulin-like peptide genes and elucidated their tissue-, stage- and sex-specific expressions in the yellow fever mosquito, Aedes aegypti •Explored the interactions between insulin-like peptides and their receptor/s in two mosquito species •Developted and validated ELISA for measuring the insulin-like peptides in two mosquito species Show less



NC State University

• Post-doctoral Research Associate

  • Jul 1999 - Jul 2004

  •Identified and purified a high dendity lipoprotein from plasma of two insect species •Developed anti-rabbit policlonal antibodies for two insect species •Developed and validated ELISA to study the transport of lipids in several cockroaches •Elucidated the gene expression, protein titration and endocrine regulation of potent human allergen, Bla g 4, in German cockroach