Experience

Regeneron

• United States
• Biotechnology
• 700 & Above Employee

• Principal Scientist

  • Jun 2023 - Present



Beckman Coulter Life Sciences

• United States
• Biotechnology Research
• 700 & Above Employee

• Staff Application Development Scientist, R&D

  • Aug 2022 - Jun 2023

  - SME/Lead scientist to support new product development - Conduct R&D, interact in a multi-discipline, international team to provide solutions to customer's pain points - Supervise and train junior scientists - Manage and coordinate timelines and resources to secure project deliverables

• Senior Application Development Scientist, R&D

  • Mar 2021 - Jul 2022



Indiana Biosciences Research Institute

• United States
• Research Services
• 1 - 100 Employee

• Senior Research Associate

  • May 2019 - Mar 2021

  Pharmaceutical Biotechnology Center - Established and implemented new technologies and platforms for pre-clinical development (IND) of therapeutic monoclonal antibodies - Antibody discovery, upstream process development - DNA vector design; transfection; expression; CHO batch and fed-batch cultures; antibody purification (AKTA and Unicorn) and characterization; Spectro Flow Cytometry Pharmaceutical Biotechnology Center - Established and implemented new technologies and platforms for pre-clinical development (IND) of therapeutic monoclonal antibodies - Antibody discovery, upstream process development - DNA vector design; transfection; expression; CHO batch and fed-batch cultures; antibody purification (AKTA and Unicorn) and characterization; Spectro Flow Cytometry



Indiana University School of Medicine

• Research Services
• 700 & Above Employee

• Research Analyst

  • May 2017 - Apr 2019

  Department of Anatomy & Cell Biology - Established and incorporated new assays, SOP, equipment for a newly established lab - Cell line development; HT flow cytometry; Chromatography (AKTA and Unicorn) - Conducted researches on Tau and Amyloid aggregation in neurodegenerative diseases - Supervised and mentored junior scientists and PhD students - Oversaw and coordinated ongoing projects Department of Anatomy & Cell Biology - Established and incorporated new assays, SOP, equipment for a newly established lab - Cell line development; HT flow cytometry; Chromatography (AKTA and Unicorn) - Conducted researches on Tau and Amyloid aggregation in neurodegenerative diseases - Supervised and mentored junior scientists and PhD students - Oversaw and coordinated ongoing projects



Regeneron

• United States
• Biotechnology
• 700 & Above Employee

• Research Associate IV

  • Sep 2012 - Apr 2017

  Protein Expression Science - Conducted R&D, implemented new technologies for the platforms from antibody discovery to upstream process development - CHO cell line development; Fed-batch culture; Cell sorting; DNA vector design; HT cloning and expression; HT automated transient transfection and expression; HT automated NGS library preparation; MiSeq and 454 NGS; Ultracentrifugation. - Present to senior leadership on weekly basis - Mentored interns and junior scientists Protein Expression Science - Conducted R&D, implemented new technologies for the platforms from antibody discovery to upstream process development - CHO cell line development; Fed-batch culture; Cell sorting; DNA vector design; HT cloning and expression; HT automated transient transfection and expression; HT automated NGS library preparation; MiSeq and 454 NGS; Ultracentrifugation. - Present to senior leadership on weekly basis - Mentored interns and junior scientists



Washington University School of Medicine in St. Louis

• Hospitals and Health Care
• 700 & Above Employee

• Staff Scientist

  • May 2006 - Sep 2012

  Division of Pulmonary and Critical Care Medicine - Conducted researches on the role of viruses in causing acute infection and chronic lung diseases - Established a new NKT-Macrophage primary co-culture system; Viruses purification and plaque assay; Multi-color cell sorting and analyzing; Cell culture; qPCR; Multi-plex assay;

• Senior Research Technician

  • Nov 1999 - May 2006

  Division of Pulmonary and Critical Care Medicine - Established a new cell culture platform and published a highly cited paper in the field up to date - A novel mouse airway epithelial cell culture system - Investigated on the mechanism of cell differentiation of ciliogenesis



TongJi Medical College of HUST

• China
• Higher Education
• 1 - 100 Employee

• Senior Technologist of Lab Medicine

  • Sep 1985 - Jul 1998

  Department of Biochemistry and Molecular Biology - Conducted researches on gene therapy of ADA-SCID; inherited metabolic disorders; development and male infertility - Led and coordinated nationwide molecular biology workshops that covered DNA recombination, gene expression and characterization. - Extensive and strong molecular biology (DNA clone, mutagenesis, PCR, Southern, Northern and Western blots, transfection), biochemistry and cell culture experience (primary cell culture);… Show more Department of Biochemistry and Molecular Biology - Conducted researches on gene therapy of ADA-SCID; inherited metabolic disorders; development and male infertility - Led and coordinated nationwide molecular biology workshops that covered DNA recombination, gene expression and characterization. - Extensive and strong molecular biology (DNA clone, mutagenesis, PCR, Southern, Northern and Western blots, transfection), biochemistry and cell culture experience (primary cell culture); Protein purification and characterization; Assay development; Enzyme kinetic study Show less Department of Biochemistry and Molecular Biology - Conducted researches on gene therapy of ADA-SCID; inherited metabolic disorders; development and male infertility - Led and coordinated nationwide molecular biology workshops that covered DNA recombination, gene expression and characterization. - Extensive and strong molecular biology (DNA clone, mutagenesis, PCR, Southern, Northern and Western blots, transfection), biochemistry and cell culture experience (primary cell culture);… Show more Department of Biochemistry and Molecular Biology - Conducted researches on gene therapy of ADA-SCID; inherited metabolic disorders; development and male infertility - Led and coordinated nationwide molecular biology workshops that covered DNA recombination, gene expression and characterization. - Extensive and strong molecular biology (DNA clone, mutagenesis, PCR, Southern, Northern and Western blots, transfection), biochemistry and cell culture experience (primary cell culture); Protein purification and characterization; Assay development; Enzyme kinetic study Show less