Experience

American Molecular Laboratories

• United States
• Biotechnology Research
• 1 - 100 Employee

• Vice President Operations

  • Mar 2022 - Present

  Greater Chicago Area

• Director of Research and Development

  • Aug 2015 - Mar 2022

  Great Chicago Area evelop and implement the cancer diagnostics based on next generation sequencing on the platforms Illumina MiSeq DX and Thermo Fisher Ion 5S to establish a clinical diagnostics reference laboratory. • Designed and validated the next generation sequencing panels for cancer mutation analysis. • Developed microbiology antibiotic resistance mutation detection and analysis panel with next generation sequencing (patent pending). • Developing microsatellite instability and Sanger… Show more evelop and implement the cancer diagnostics based on next generation sequencing on the platforms Illumina MiSeq DX and Thermo Fisher Ion 5S to establish a clinical diagnostics reference laboratory. • Designed and validated the next generation sequencing panels for cancer mutation analysis. • Developed microbiology antibiotic resistance mutation detection and analysis panel with next generation sequencing (patent pending). • Developing microsatellite instability and Sanger sequencing to detect and validate mutations on ABI 3500 for colon cancer diagnostics. • Leading and managing the R&D on NGS, maintenance SOP and oversee the daily laboratory activities.



Celsee Diagnostics Inc.

• Research Scientist and R&D Manager in Assay Development

  • Dec 2012 - Jul 2015

  Detroit, Michigan Focus on R&D in molecular assays for next generation diagnostics of cancer. • Design and implement immunofluorescence assay to characterize circulating tumor cells (CTCs) captured by microfluidic device from blood. • Create and establish fluorescence in situ hybridization (FISH) and in situ PCR to distinguish single cancer cells (CTCs) on the microfluidic chip. • Introduce RNA FISH to analyze cancer associate gene expression and to identify cancer stem cell from circulating… Show more Focus on R&D in molecular assays for next generation diagnostics of cancer. • Design and implement immunofluorescence assay to characterize circulating tumor cells (CTCs) captured by microfluidic device from blood. • Create and establish fluorescence in situ hybridization (FISH) and in situ PCR to distinguish single cancer cells (CTCs) on the microfluidic chip. • Introduce RNA FISH to analyze cancer associate gene expression and to identify cancer stem cell from circulating tumor cells (CTCs). • Develop next generation sequencing of single cell (circulating tumor cells). Show less



Cornell University

• United States
• Higher Education
• 700 & Above Employee

• Research Associate

  • Nov 2006 - Nov 2012

  Ithaca, New York Area • Led the project of molecular mechanism of preeclampsia using the BPH/5 genetic model mouse. • Designed and implemented experiments utilizing state-of-the-art molecular biology technologies for genome-wide gene and microRNAs expression profiling of mouse model, and provide advanced data analysis as well as trouble shooting. • Revealed and validated the function of Thrombospondin-2 (angiogenesis inhibitor) in human preeclampsia. • Established DNA microarray, real time qPCR and in… Show more • Led the project of molecular mechanism of preeclampsia using the BPH/5 genetic model mouse. • Designed and implemented experiments utilizing state-of-the-art molecular biology technologies for genome-wide gene and microRNAs expression profiling of mouse model, and provide advanced data analysis as well as trouble shooting. • Revealed and validated the function of Thrombospondin-2 (angiogenesis inhibitor) in human preeclampsia. • Established DNA microarray, real time qPCR and in situ hybridization (prone design and generation, hybridization experiment optimization) technologies in the lab. Show less



Lady Davis Institute for Medical Research, Jewish General Hospital/McGill University

• Research Associate

  • Jan 2005 - Nov 2006

  Montreal, Canada Area • In a cancer research lab, my research projects focus on FAK and ErBb signing transduction pathways and function analysis in breast cancer invasion and metastasis in mouse model and cell lines using genomics tools, Affymetrix chips to analyze genome-wide gene expression profiling. • The dysregulated genes were confirmed with real-time qPCR and Western blot and function analysis using siRNA.



Institut de recherches cliniques de Montréal (IRCM) / Montreal Clinical Research Institute

• Canada
• Research Services
• 200 - 300 Employee

• Functional Genomics Platform Manager

  • Jun 2003 - Jan 2005

  Montreal, Canada Area • Established the microarray core facilities, developed and optimized the protocols for the DNA microarray system for using in gene expression and genome-wide location analysis (ChIP-Chip). • Developed human and mouse promoter arrays for analyzing the DNA-protein interaction in human and mouse genome. • Oversee the service of molecular biology and genomics, including microarray core facilities, Agilent 2100 Bioanalyzer, DNA sequencing, real-time qPCR and microsatellites… Show more • Established the microarray core facilities, developed and optimized the protocols for the DNA microarray system for using in gene expression and genome-wide location analysis (ChIP-Chip). • Developed human and mouse promoter arrays for analyzing the DNA-protein interaction in human and mouse genome. • Oversee the service of molecular biology and genomics, including microarray core facilities, Agilent 2100 Bioanalyzer, DNA sequencing, real-time qPCR and microsatellites genotyping. • Organized daily activities and operation and supervised three lab technicians. Effectively communicated with institute communities, research scientists, biotech companies. Show less



Cereal Research Center, Agriculture and Agri-Food Canada

• NSERC Visiting Fellow

  • Jun 2001 - Jun 2003

  • Discovery of single nucleotide polymorphisms (SNPs) from sequencing of expressed genes of wheat and validated by allelic specific primer extension on ABI 3100 genetic analyzer to identify Canadian wheat. • Developed a high-throughput genotyping platform of single nucleotide polymorphism using invader assay and DNA chips. • Discovered novel FHB resistance genes and analyzed gene expression profiling with cDNA microarray.



Texas A&M University

• United States
• Higher Education
• 700 & Above Employee

• Assistant Research Scientist

  • Jul 1999 - Jun 2001

  • Developed 200 microsatellites as molecular markers from pine genome. • Constructed pine genetic mapping and QTL analysis with microsatellites. • Revealed the genetic load happened in pine trees with microsatellites.



Université Laval

• Canada
• Higher Education
• 700 & Above Employee

• Postdoctoral Research Fellow

  • Oct 1997 - Jun 1999

  Quebec City, Quebec Canada • Developed RADP markers from White and Black spruce tree genome. • Constructed genetic mapping using RAPD and STS markers for QTL analysis.