Experience

Locanabio, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Associate I

  • Aug 2022 - Present

  Develop and optimize multiple ddPCR and qRT-PCR assays to evaluate transgene expression and vector genome biodistribution of proprietary RNA targeting gene therapy constructs. Assess the efficacy of RNA-binding proteins via MSD assays screening for disease phenotype reduction as well as expression of the therapeutics. Collaborate with multiple teams and work cross-functionally to support preclinical and IND enabling studies for the Duchenne Muscular Dystrophy program through downstream… Show more Develop and optimize multiple ddPCR and qRT-PCR assays to evaluate transgene expression and vector genome biodistribution of proprietary RNA targeting gene therapy constructs. Assess the efficacy of RNA-binding proteins via MSD assays screening for disease phenotype reduction as well as expression of the therapeutics. Collaborate with multiple teams and work cross-functionally to support preclinical and IND enabling studies for the Duchenne Muscular Dystrophy program through downstream analyses of in vivo samples. Facilitate test method transfer to contract research organizations for large-scale pilot pharmacology studies using nonhuman primates and mouse models. Create excel templates for 96-well format assays that reduces over 80% of redundant workload via automation. Establish and maintain multiple electronic lab notebook templates for efficient and consistent documentation.

• Research Associate II

  • Nov 2021 - Aug 2022



Salk Institute for Biological Studies

• United States
• Research Services
• 700 & Above Employee

• Research Assistant I

  • Mar 2021 - Nov 2021

  Performed mammary orthotopic transplantation in immunocompromised mice to assess how tumorigenesis and tumor progression differ per donor mouse strains and/or health conditions. Utilized fluorescence-activated cell sorting (FACS) to measure self-renewal and differentiation capacity of mammary epithelium during mammary gland reconstitution process. Renovated mouse genotyping structure to allow expedited management of more than 5000 mice per year by standardizing PCR protocols and reducing… Show more Performed mammary orthotopic transplantation in immunocompromised mice to assess how tumorigenesis and tumor progression differ per donor mouse strains and/or health conditions. Utilized fluorescence-activated cell sorting (FACS) to measure self-renewal and differentiation capacity of mammary epithelium during mammary gland reconstitution process. Renovated mouse genotyping structure to allow expedited management of more than 5000 mice per year by standardizing PCR protocols and reducing repetitive procedures. Enhance viral titer of lentivirus via transgene size reduction and produce chemically competent cells with 107 cfu/ug transformation efficiency. Established structured training protocols and assessment forms, and produced videography of laboratory and surgical procedures for instructional purposes to facilitate new technician training programs.

• Laboratory Technician

  • Dec 2019 - Mar 2021



Abbott

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Assistant I

  • Oct 2018 - Sep 2019

  Investigated the optimal ratio of gold to antibody conjugation on lateral flow assay to improve the accuracy of drug screening device. Performed daily confirmation tests on 90 to 100 lots of current market products to identify areas for in-process improvement. Maintained an organized and detailed record of experiments in a laboratory notebook in accordance with the Good Documentation Practices guideline and QA regulations. Awarded “Certificate of Recognition” for the work on eCup E&I… Show more Investigated the optimal ratio of gold to antibody conjugation on lateral flow assay to improve the accuracy of drug screening device. Performed daily confirmation tests on 90 to 100 lots of current market products to identify areas for in-process improvement. Maintained an organized and detailed record of experiments in a laboratory notebook in accordance with the Good Documentation Practices guideline and QA regulations. Awarded “Certificate of Recognition” for the work on eCup E&I and continued development of Chronos. Show less Investigated the optimal ratio of gold to antibody conjugation on lateral flow assay to improve the accuracy of drug screening device. Performed daily confirmation tests on 90 to 100 lots of current market products to identify areas for in-process improvement. Maintained an organized and detailed record of experiments in a laboratory notebook in accordance with the Good Documentation Practices guideline and QA regulations. Awarded “Certificate of Recognition” for the work on eCup E&I… Show more Investigated the optimal ratio of gold to antibody conjugation on lateral flow assay to improve the accuracy of drug screening device. Performed daily confirmation tests on 90 to 100 lots of current market products to identify areas for in-process improvement. Maintained an organized and detailed record of experiments in a laboratory notebook in accordance with the Good Documentation Practices guideline and QA regulations. Awarded “Certificate of Recognition” for the work on eCup E&I and continued development of Chronos. Show less