Yann Huon de Kermadec

Research Engineer at Learning Planet Institute
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Contact Information
us****@****om
(386) 825-5501
Location
FR
Languages
  • Francais Native or bilingual proficiency
  • English Professional working proficiency

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Bio

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Experience

    • France
    • Higher Education
    • 100 - 200 Employee
    • Research Engineer
      • Dec 2021 - Present

      Development of a low-cost high-throughput method to select high-expressing pichia pastoris strain Development of a low-cost high-throughput method to select high-expressing pichia pastoris strain

    • Research Scientist
      • Nov 2019 - Aug 2021

      Development of bioreactor process to scale up the production of protein in pichia pastoris. Development of bioreactor process to scale up the production of protein in pichia pastoris.

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Research Scientist
      • Mar 2017 - Nov 2019

      I put into operation a Waters 600 HPLC (change tubing, prime the pumps) The lab is accessible to everyone who want to start a biological project. I help and train people to start their own project, providing technical support and scientific advice. I join an exciting project aimed to provide a low cost production protocol for Insulin. I'm currently working on a refolding strategy to purify this protein. I put into operation a Waters 600 HPLC (change tubing, prime the pumps) The lab is accessible to everyone who want to start a biological project. I help and train people to start their own project, providing technical support and scientific advice. I join an exciting project aimed to provide a low cost production protocol for Insulin. I'm currently working on a refolding strategy to purify this protein.

  • Communauté Université Grenoble Alpes
    • Région de Grenoble, France
    • PHD
      • Oct 2011 - Nov 2015

      My project was to study the membrane protein BmrA with the nanodiscs methods. I was responsible of: - Over-expression of membrane protein in bacteria or cell-free (hAAC1) and soluble protein (MSP1, MSP1ED1) - Development of membrane protein purification (BmrA, YedZ) - Reconstitution of membrane protein in nanodiscs (hAAC1, BmrA, BmrCD, YedZ, PopB, FhuA, 5HT3a, spNOX) - Characterization of the nanodiscs (size-exclusion chromatography) - Activity test (ATPase activity with BmrA) - Electron microscopy and cryo-EM (in collaboration) (BmrA, PopB)

Education

  • Université Pierre et Marie Curie (Paris VI)
    Master's degree, Biochimie
    2009 - 2011
  • Université de Versailles Saint-Quentin-en-Yvelines
    Bachelor degree, Biochemistry

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