William Crowther
Application Scientist at Oxford Nanopore Technologies- Claim this Profile
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Experience
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Oxford Nanopore Technologies
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United Kingdom
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Biotechnology Research
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700 & Above Employee
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Application Scientist
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Mar 2022 - Present
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Rosalind Franklin Laboratory
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United Kingdom
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Government Relations Services
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1 - 100 Employee
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Senior Scientist
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May 2021 - Mar 2022
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University of Warwick
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United Kingdom
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Higher Education
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700 & Above Employee
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Sep 2016 - Mar 2022
Developing novel NGS methods for rapidly mapping resistance genes directly from diverse Genebank crop varieties.
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Nov 2015 - Sep 2016
• Working alongside PhD students, primarily to characterise and map disease resistance in Brassicas. • Coordinating pathology experiments (inoculating and phenotyping), and responsibility for maintaining biotrophic isolates (Albugo candida).• Use of a wide array of lab techniques; including DNA extractions, PCR, Long Range PCR, restriction digests, RNA extractions, qPCR, trypan blue staining and assembling CRISPR constructs.
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Queen Mary University of London
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United Kingdom
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Higher Education
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700 & Above Employee
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Research Technician
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Oct 2014 - Oct 2015
• Processing samples of global ash species for analysis of genetic variation and presence of genes conferring low susceptibility to ash dieback. • High quality DNA extractions, PCR (ITS + microsatellite markers), preparation of DNA for WGS and flow cytometry (genome size measurements). • Preliminary analysis of WGS data – training in genome assemblies. • Processing samples of global ash species for analysis of genetic variation and presence of genes conferring low susceptibility to ash dieback. • High quality DNA extractions, PCR (ITS + microsatellite markers), preparation of DNA for WGS and flow cytometry (genome size measurements). • Preliminary analysis of WGS data – training in genome assemblies.
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University of Oxford
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United Kingdom
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Research Services
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700 & Above Employee
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Research Technician
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Apr 2013 - Jul 2014
• Investigating the drivers behind bleeding canker disease in UK horse chestnut trees. • Four months field work around the UK; collecting samples and measuring environmental covariates. • Protocol management and budgeting. • Laboratory based DNA extractions (from wood), PCR, sanger sequencing and cell culturing. • Investigating the drivers behind bleeding canker disease in UK horse chestnut trees. • Four months field work around the UK; collecting samples and measuring environmental covariates. • Protocol management and budgeting. • Laboratory based DNA extractions (from wood), PCR, sanger sequencing and cell culturing.
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Education
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University of Warwick
Doctor of Philosophy - PhD, Molecular plant pathology -
Imperial College London
Master of Science - MS, Ecology, Evolution and Conservation -
Univeristy of Liverpool
Bachelor of Science - BS, Environmental Biology