William Crowther

Application Scientist at Oxford Nanopore Technologies
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Location
Leamington Spa, England, United Kingdom, UK

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Experience

    • United Kingdom
    • Biotechnology Research
    • 700 & Above Employee
    • Application Scientist
      • Mar 2022 - Present
    • United Kingdom
    • Government Relations Services
    • 1 - 100 Employee
    • Senior Scientist
      • May 2021 - Mar 2022
    • United Kingdom
    • Higher Education
    • 700 & Above Employee
      • Sep 2016 - Mar 2022

      Developing novel NGS methods for rapidly mapping resistance genes directly from diverse Genebank crop varieties.

      • Nov 2015 - Sep 2016

      • Working alongside PhD students, primarily to characterise and map disease resistance in Brassicas. • Coordinating pathology experiments (inoculating and phenotyping), and responsibility for maintaining biotrophic isolates (Albugo candida).• Use of a wide array of lab techniques; including DNA extractions, PCR, Long Range PCR, restriction digests, RNA extractions, qPCR, trypan blue staining and assembling CRISPR constructs.

    • United Kingdom
    • Higher Education
    • 700 & Above Employee
    • Research Technician
      • Oct 2014 - Oct 2015

      • Processing samples of global ash species for analysis of genetic variation and presence of genes conferring low susceptibility to ash dieback. • High quality DNA extractions, PCR (ITS + microsatellite markers), preparation of DNA for WGS and flow cytometry (genome size measurements). • Preliminary analysis of WGS data – training in genome assemblies. • Processing samples of global ash species for analysis of genetic variation and presence of genes conferring low susceptibility to ash dieback. • High quality DNA extractions, PCR (ITS + microsatellite markers), preparation of DNA for WGS and flow cytometry (genome size measurements). • Preliminary analysis of WGS data – training in genome assemblies.

    • United Kingdom
    • Research Services
    • 700 & Above Employee
    • Research Technician
      • Apr 2013 - Jul 2014

      • Investigating the drivers behind bleeding canker disease in UK horse chestnut trees. • Four months field work around the UK; collecting samples and measuring environmental covariates. • Protocol management and budgeting. • Laboratory based DNA extractions (from wood), PCR, sanger sequencing and cell culturing. • Investigating the drivers behind bleeding canker disease in UK horse chestnut trees. • Four months field work around the UK; collecting samples and measuring environmental covariates. • Protocol management and budgeting. • Laboratory based DNA extractions (from wood), PCR, sanger sequencing and cell culturing.

Education

  • University of Warwick
    Doctor of Philosophy - PhD, Molecular plant pathology
    2016 - 2021
  • Imperial College London
    Master of Science - MS, Ecology, Evolution and Conservation
    2010 - 2011
  • Univeristy of Liverpool
    Bachelor of Science - BS, Environmental Biology
    2005 - 2009

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