Wade Johnson
Manager, Assay Development at R&D Systems- Claim this Profile
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Topline Score
Bio
Experience
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R&D Systems
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United States
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Biotechnology Research
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300 - 400 Employee
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Manager, Assay Development
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Feb 2022 - Present
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UNM Flow Cytometry Core
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Albuquerque, New Mexico
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Manager
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May 2018 - Feb 2022
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Richard Cripps lab, University of New Mexico
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Albuquerque, New Mexico Area
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Postdoctoral Researcher
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Jul 2017 - May 2018
Through use of CRISPR technology we are tagging specific genes as well as creating novel mutations to better understand the molecular and biochemical changes that occur during muscle differentiation and development in Drosophila melanogaster. Through use of CRISPR technology we are tagging specific genes as well as creating novel mutations to better understand the molecular and biochemical changes that occur during muscle differentiation and development in Drosophila melanogaster.
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UNM Health Sciences Center
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Albuquerque, New Mexico Area
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Scientist
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May 2016 - Mar 2017
• Used various methodologies to characterize the relationship between immune system and cancer cells. Western blot, Q-PCR, Flow Cytometry, T Cell and NK Cell Isolation • Developed protocols for isolation and characterization of murine splenocytes, bone marrow macrophages and tumor samples • Isolated human tumor samples for analysis of cytokines, RNA and protein • Trained lab members in use of flow cytometer, western blot techniques, tissue isolation, tissue processing and RNA extraction Show less
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iQue® - Advanced Flow Cytometry Platform
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Biotechnology
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1 - 100 Employee
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Senior Scientist
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Jun 2014 - Oct 2015
IntelliCyt developed the iQue, a high throughput flow cytometer capable of sampling 96, 384 and 1536 well plates in as little as 5, 20 and 80 minutes, respectively. Developed assays in key areas of the immunology field including, immuno-phenotyping, natural killer cell cytotoxicity, cytokine detection, phosphorylation-flow assays and apoptosis characterization IntelliCyt developed the iQue, a high throughput flow cytometer capable of sampling 96, 384 and 1536 well plates in as little as 5, 20 and 80 minutes, respectively. Developed assays in key areas of the immunology field including, immuno-phenotyping, natural killer cell cytotoxicity, cytokine detection, phosphorylation-flow assays and apoptosis characterization
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R&D Systems
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United States
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Biotechnology Research
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300 - 400 Employee
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Scientist II
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Mar 2006 - Apr 2014
Validated stem cell products through ICC, IHC, Flow Cytometry, Western Blot, Antibody Arrays, and ELISA. These products included stem cell kits, reagents and antibodies. Coordinated antibody characterization and validation for department. Maintained stem cell cultures and cell lines. Instrumental in implementation of high throughput methodologies in antibody characterization and validation. Validated stem cell products through ICC, IHC, Flow Cytometry, Western Blot, Antibody Arrays, and ELISA. These products included stem cell kits, reagents and antibodies. Coordinated antibody characterization and validation for department. Maintained stem cell cultures and cell lines. Instrumental in implementation of high throughput methodologies in antibody characterization and validation.
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Universtiy of Boulder, CO
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Boulder, CO
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Post-doc
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1999 - 2006
Identification of factors involved in C.elegans vulval development Identification of factors involved in C.elegans vulval development
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Northwestern University - The Feinberg School of Medicine
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United States
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Higher Education
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700 & Above Employee
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Doctoral Research
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1993 - 1999
Regulation of human chorionic gonadotropin gene expression during placental development Regulation of human chorionic gonadotropin gene expression during placental development
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Upsher-Smith
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Pharmaceutical Manufacturing
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300 - 400 Employee
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Methods Development Chemist
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1992 - 1993
Develop methods and SOPs for a generic drug company Develop methods and SOPs for a generic drug company
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University of Illinois Chicago
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United States
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Higher Education
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700 & Above Employee
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Research Technician
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1990 - 1993
Drosophila gene regulation during adipose tissue development Drosophila gene regulation during adipose tissue development
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Laboratory Consultants
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Tempe, AZ
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Laboratory Technician
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1989 - 1990
Characterize and quantify chemical composition of soil and plant samples Characterize and quantify chemical composition of soil and plant samples
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Education
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Northwestern University
PhD, Molecular Biology/Genetics