Wade Johnson

Manager, Assay Development at R&D Systems
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Contact Information
us****@****om
(386) 825-5501
Location
US

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Experience

    • United States
    • Biotechnology Research
    • 300 - 400 Employee
    • Manager, Assay Development
      • Feb 2022 - Present

  • UNM Flow Cytometry Core
    • Albuquerque, New Mexico
    • Manager
      • May 2018 - Feb 2022

    • Postdoctoral Researcher
      • Jul 2017 - May 2018

      Through use of CRISPR technology we are tagging specific genes as well as creating novel mutations to better understand the molecular and biochemical changes that occur during muscle differentiation and development in Drosophila melanogaster. Through use of CRISPR technology we are tagging specific genes as well as creating novel mutations to better understand the molecular and biochemical changes that occur during muscle differentiation and development in Drosophila melanogaster.

  • UNM Health Sciences Center
    • Albuquerque, New Mexico Area
    • Scientist
      • May 2016 - Mar 2017

      • Used various methodologies to characterize the relationship between immune system and cancer cells. Western blot, Q-PCR, Flow Cytometry, T Cell and NK Cell Isolation • Developed protocols for isolation and characterization of murine splenocytes, bone marrow macrophages and tumor samples • Isolated human tumor samples for analysis of cytokines, RNA and protein • Trained lab members in use of flow cytometer, western blot techniques, tissue isolation, tissue processing and RNA extraction Show less

    • Biotechnology
    • 1 - 100 Employee
    • Senior Scientist
      • Jun 2014 - Oct 2015

      IntelliCyt developed the iQue, a high throughput flow cytometer capable of sampling 96, 384 and 1536 well plates in as little as 5, 20 and 80 minutes, respectively. Developed assays in key areas of the immunology field including, immuno-phenotyping, natural killer cell cytotoxicity, cytokine detection, phosphorylation-flow assays and apoptosis characterization IntelliCyt developed the iQue, a high throughput flow cytometer capable of sampling 96, 384 and 1536 well plates in as little as 5, 20 and 80 minutes, respectively. Developed assays in key areas of the immunology field including, immuno-phenotyping, natural killer cell cytotoxicity, cytokine detection, phosphorylation-flow assays and apoptosis characterization

    • United States
    • Biotechnology Research
    • 300 - 400 Employee
    • Scientist II
      • Mar 2006 - Apr 2014

      Validated stem cell products through ICC, IHC, Flow Cytometry, Western Blot, Antibody Arrays, and ELISA. These products included stem cell kits, reagents and antibodies. Coordinated antibody characterization and validation for department. Maintained stem cell cultures and cell lines. Instrumental in implementation of high throughput methodologies in antibody characterization and validation. Validated stem cell products through ICC, IHC, Flow Cytometry, Western Blot, Antibody Arrays, and ELISA. These products included stem cell kits, reagents and antibodies. Coordinated antibody characterization and validation for department. Maintained stem cell cultures and cell lines. Instrumental in implementation of high throughput methodologies in antibody characterization and validation.

    • Post-doc
      • 1999 - 2006

      Identification of factors involved in C.elegans vulval development Identification of factors involved in C.elegans vulval development

    • United States
    • Higher Education
    • 700 & Above Employee
    • Doctoral Research
      • 1993 - 1999

      Regulation of human chorionic gonadotropin gene expression during placental development Regulation of human chorionic gonadotropin gene expression during placental development

    • Pharmaceutical Manufacturing
    • 300 - 400 Employee
    • Methods Development Chemist
      • 1992 - 1993

      Develop methods and SOPs for a generic drug company Develop methods and SOPs for a generic drug company

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Technician
      • 1990 - 1993

      Drosophila gene regulation during adipose tissue development Drosophila gene regulation during adipose tissue development

    • Laboratory Technician
      • 1989 - 1990

      Characterize and quantify chemical composition of soil and plant samples Characterize and quantify chemical composition of soil and plant samples

Education

  • Northwestern University
    PhD, Molecular Biology/Genetics
    1992 - 1999

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