Scientist

• Apr 2022 - Present

Senior Research Associate

• Oct 2020 - Mar 2022

• Expanded and differentiated erythropoietic progenitor cells (EPCs) from G-CSF mobilized CD34+ stem/progenitor cells. • Characterized EPCs using flow cytometry and qPCR to assess erythroid lineage commitment. • Screened PROTAC-based degraders to target specific proteins in EPCs. • Conducted gene knockout studies using CRISPR-Cas9 and siRNA techniques for target validation. • Performed small molecules screening using high-content imaging for mitochondrial morphology and autophagy assays. Show less

Senior Associate Scientist

• Oct 2019 - Sep 2020

• Optimized conditions for lentiviral transduction and electroporation of αβ and gd cells for CAR-T cell engineering.• Assessed CAR-T phenotype through multi-parameter flow cytometry.• Evaluated CAR-T cell function through target cell killing assays, cytokine secretion and proliferation assays.• Standardized the protocol for monoclonal-antibody-based expansion of gd T cells for adoptive T cell therapy.• Conducted flow cytometry-based assays to quantify the binding efficiency of bispecific antibodies to T cells and tumor-associated antigen (TAA) expressing cancer cells.• Bispecific antibody-mediated cytotoxicity assays against cancer cells co-cultured with T cells using flow cytometry and Incucyte-based killing assays.• Evaluated bispecific antibody-induced activation, proliferation (CTV/CFSE dilution), and cytokine profiles in healthy volunteers and AML patients' PBMCs.• Isolated tumor-infiltrating lymphocytes (TIL) from tumor samples and performed phenotypic analysis.• Reprogrammed PBMCs using Cyto-tune iPSC 2.0 Sendai reprogramming kit to generate induced pluripotent stem cells (iPSCs).• Maintained iPSC lines, performed passaging, and assessed pluripotency through lineage differentiation, flow staining, and IHC. Show less

Associate Scientist

• Sep 2017 - Sep 2019

Research Associate II

• Aug 2015 - Aug 2017

• Differentiated mouse bone marrow cells to macrophages (BMDM) for phagocytosis assay. • Screened large molecules as immune checkpoint blockers. • Enumerated T cells proliferation by CFSE/CTV dilution. • Conducted cellular target engagement assays for small molecules to elucidate target occupancy. • Assessed cell cycle progression and apoptosis by flow cytometry. • Evaluated the effect of small molecules on target-specific protein modulation by Western blot. • Determined the anti-proliferative effect of New Chemical Entities (NCEs). Show less

Research Associate

• Jun 2012 - Jul 2015

Developing and validation of cell-based assays for osteoarthritis, Immuno-modulation studies. Developing and validation of cell-based assays for osteoarthritis, Immuno-modulation studies.