Experience

Axxam

• Italy
• Biotechnology Research
• 100 - 200 Employee

• Cell Biology Scientist

  • Apr 2020 - Present



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Postdoctoral Research Fellow

  • Jul 2016 - Feb 2020

  Lab K.S. Wendt, Cell Biology Department I investigated the role of Cohesin complex and Cohesin regulator in the genome organisation, with a particular focus on disease conditions like genetic disorders and cancer. I coached young scientists (undergraduate and PhD student). Skills: • CRISPR/Cas9 genome editing (Knock-in, Knock-out) • ASO (Antisense oligonucleotides) • RNA sequencing • 4C / Hi-C analysis • ChIP-sequencing • Cell biology • Molecular Biology (DNA, RNA, DNA cloning) • Biochemistry • Immunoprecipitation • Chromatin Immunoprecipitation (ChIP) • Flowcytometry Cell Cycle Analysis Show less



San Raffaele Biomedical Science Park

• Hospitals and Health Care
• 1 - 100 Employee

• Postdoctoral Researcher

  • Apr 2015 - May 2016

  Lab D. Gabellini.I explored the complex interaction between the epigenetic and the genetic components in FSHD muscular dystrophy, a genetic disorder caused by a reduced copy number of D4Z4 macrosatellite repeat. Based on my previous work on the role of Polycomb in the disease, I answered to a key question in the field: How does D4Z4 repeat copy number influence the severity of FSHD?Skills:• DNA Cloning of Complex Fragments (High GC, Repetitive, Large fragments)• Cell biology• Site-specific integration (Flp / Frt Recombination)• Flow Cytometry Analyses (Two Colours)• DNA Methylation Analyses (Bisulfite Conversion; Methylated DNA immunoprecipitation -MeDIP) • Chromatin Immunoprecipitation (ChIP)• Molecular Biology (RNA and DNA) Show less

• Doctoral Student

  • Jan 2010 - Apr 2015

  Lab D. Gabellini.I unveiled the role played by the Polycomb group of epigenetic factors in a genetic disease, the FSHD muscular dystrophy, which is caused by a reduced copy number of the D4Z4 macrosatellite repeat. In particular, my major question was:How a reduced repeat copy number can influence the transcriptional activity of the FSHD locus, thus determining the disease?Skills:• Cell biology (Human and Murine Primary cells and Cell lines)• Generation of Transient and Stable cell lines (Transfection; Lentiviral Infection; Site-Specific Integration via Flp/Frt Recombination)• Molecular Biology (RNA - Genomic DNA - Plasmid DNA - BAC DNA) • DNA cloning techniques• PCR - qPCR - RTqPCR • Analyses of complex sequences (High GC, Repetitive, Large fragments)• Chromatin Immunoprecipitation (ChIP) of Histone Modifications and Transcription Factors• Combined Technique for DNA Methylation Analyses of ChIP-DNA (ChIP-Bisulfite Sequencing) Show less



LMU - Ludwig-Maximilians-Universität München

• Munich, Germany

• Guest Research Fellow

  • Dec 2009 - Dec 2009

  Collaboration wih Prof. Dr. Heinrich Leonhardt lab to learn their novel technology (DNA:protein competition binding assay - Frauer et al., NAR 2009). Collaboration wih Prof. Dr. Heinrich Leonhardt lab to learn their novel technology (DNA:protein competition binding assay - Frauer et al., NAR 2009).



Technische Universität Darmstadt

• Germany
• Higher Education
• 700 & Above Employee

• Research Fellow

  • Jan 2009 - Dec 2009

  Lab of M.C. Cardoso. I studied the protein-protein interaction and the DNA binding activity of the Methyl-CpG binding proteins, and in particular of MeCP2, epigenetic factor which is mutated in Rett Syndrome genetic disease. Skills: • Production of recombinant proteins • Immunoprecipation assays, SDS-PAGE, Western Blotting. • Mobility shift (EMSA) assays • DNA:protein competition binding assay and detection by fluorescence spectroscopy Lab of M.C. Cardoso. I studied the protein-protein interaction and the DNA binding activity of the Methyl-CpG binding proteins, and in particular of MeCP2, epigenetic factor which is mutated in Rett Syndrome genetic disease. Skills: • Production of recombinant proteins • Immunoprecipation assays, SDS-PAGE, Western Blotting. • Mobility shift (EMSA) assays • DNA:protein competition binding assay and detection by fluorescence spectroscopy