Principal Scientist PD & CMC lead

• 2022 - Present

Manufacturing Manager

• Nov 2021 - Feb 2022

Senior Scientist / Team lead

• 2020 - Nov 2021

Manufacturing Scientist

• 2019 - 2020

Manufacturing genetically modified T cells

Research Scientist

• 2018 - 2019

Cell and Gene therapy Cell and Gene therapy

PhD Researcher

• 2013 - 2017

PhD synopsis: Using a mouse fibroblast model synchronized by contact inhibition and serum starvation, and combination of siRNA mediated depletion of cyclins and chemical inhibition of CDK activity at restriction point we have monitored replication complex assembly in vivo and in vitro. Successfully developed and analyzed cell free mediated DNA replication assay with G1 nuclei and cyclin depleted nuclei. S phase extract when supplemented with nuclei can initiate DNA replication, however, when… Show more PhD synopsis: Using a mouse fibroblast model synchronized by contact inhibition and serum starvation, and combination of siRNA mediated depletion of cyclins and chemical inhibition of CDK activity at restriction point we have monitored replication complex assembly in vivo and in vitro. Successfully developed and analyzed cell free mediated DNA replication assay with G1 nuclei and cyclin depleted nuclei. S phase extract when supplemented with nuclei can initiate DNA replication, however, when cyclin E depleted nuclei failed to initiate DNA replication even in presence of S phase extract. These contrasts with Cyclin A depleted nuclei that show a reduction in pre-RC proteins Cdc6 and Mcm2 loading on chromatin that can be reversed by addition of S-phase cytosolic extracts, recapitulating initiation of DNA replication. This data suggests that sustained cyclin activity is required to maintain pre-RC protein levels and facilitate initiation of DNA replication and G1/S transition.

Laboratory Demonstrator

• 2013 - 2017

I have had opportunity to be part of science outreach programs and community day celebrations demonstrating fascinating lab experiments to children and encouraging their interests in sciences. I have also demonstrated for confocal microscope during university open days and encouraged undergraduate student admissions. I have been actively involved in teaching and demonstrating practical of various undergraduate and postgraduate modules. I have also been involved in supervision of undergraduate… Show more I have had opportunity to be part of science outreach programs and community day celebrations demonstrating fascinating lab experiments to children and encouraging their interests in sciences. I have also demonstrated for confocal microscope during university open days and encouraged undergraduate student admissions. I have been actively involved in teaching and demonstrating practical of various undergraduate and postgraduate modules. I have also been involved in supervision of undergraduate dissertation projects, designing and implementing experiments individually.

Postgraduate Research Student

• 2012 - 2013

MRes Genetics/ Molecular Cell Biology Thesis title: Investigation of relationship between DEF-6 and mRNA translation in T-cells MRes Genetics/ Molecular Cell Biology Thesis title: Investigation of relationship between DEF-6 and mRNA translation in T-cells

Junior Research Fellow

• 2012 - 2012

DNA-Protein Interactions using Atomic Force Microscopy: I was involved in the development of protocol to visualize DNA and Protein interaction under Atomic force microscopy. During my time at TIFR Mumbai, I successfully developed protocol to visualize Linear and circular plasmid DNA under liquid and semi solid conditions of atomic force microscopy. I was successful in introducing a DNA binding protein (RPA) with linear Plasmid DNA and image it under liquid conditions in atomic force… Show more DNA-Protein Interactions using Atomic Force Microscopy: I was involved in the development of protocol to visualize DNA and Protein interaction under Atomic force microscopy. During my time at TIFR Mumbai, I successfully developed protocol to visualize Linear and circular plasmid DNA under liquid and semi solid conditions of atomic force microscopy. I was successful in introducing a DNA binding protein (RPA) with linear Plasmid DNA and image it under liquid conditions in atomic force microscope. I was also involved in maintaining and documenting experiments within the Lab. Show less DNA-Protein Interactions using Atomic Force Microscopy: I was involved in the development of protocol to visualize DNA and Protein interaction under Atomic force microscopy. During my time at TIFR Mumbai, I successfully developed protocol to visualize Linear and circular plasmid DNA under liquid and semi solid conditions of atomic force microscopy. I was successful in introducing a DNA binding protein (RPA) with linear Plasmid DNA and image it under liquid conditions in atomic force… Show more DNA-Protein Interactions using Atomic Force Microscopy: I was involved in the development of protocol to visualize DNA and Protein interaction under Atomic force microscopy. During my time at TIFR Mumbai, I successfully developed protocol to visualize Linear and circular plasmid DNA under liquid and semi solid conditions of atomic force microscopy. I was successful in introducing a DNA binding protein (RPA) with linear Plasmid DNA and image it under liquid conditions in atomic force microscope. I was also involved in maintaining and documenting experiments within the Lab. Show less

Research Trainee

• 2011 - Jul 2011

‘Heterologous Expression and Nuclear localization of DNA- Binding Proteins in Human and Drosophila cells’. During this project I had an opportunity to work with a rare Chromosome Binding Protein. My work involved cloning this protein in a GFP- Overexpression vector and transfecting it in human colorectal cancer cell line. Apart from cloning, to study nuclear localization of this protein Immunofluorescence assay was also performed on Drosophila cell line. In an Independent project on another DNA… Show more ‘Heterologous Expression and Nuclear localization of DNA- Binding Proteins in Human and Drosophila cells’. During this project I had an opportunity to work with a rare Chromosome Binding Protein. My work involved cloning this protein in a GFP- Overexpression vector and transfecting it in human colorectal cancer cell line. Apart from cloning, to study nuclear localization of this protein Immunofluorescence assay was also performed on Drosophila cell line. In an Independent project on another DNA Binding Protein RNA Polymerase II was carried out. Inhibitor studies were performed on this protein; MTT assay and Immunofluorescence assay were performed to study effects of RNA Polymerase II inhibitors. Confocal Microscopy helped study Immunofluorescence assay. During this project, I had a rare opportunity to interact with a scientist from Sweden. This project helped me learning basic techniques including microbial techniques, PCR, Cloning, Electrophoresis, Polyacrylamide SDS Gel Electrophoresis, and Western Blot. Cell Culture techniques including Human, Mouse and Drosophila cells. Show less ‘Heterologous Expression and Nuclear localization of DNA- Binding Proteins in Human and Drosophila cells’. During this project I had an opportunity to work with a rare Chromosome Binding Protein. My work involved cloning this protein in a GFP- Overexpression vector and transfecting it in human colorectal cancer cell line. Apart from cloning, to study nuclear localization of this protein Immunofluorescence assay was also performed on Drosophila cell line. In an Independent project on another DNA… Show more ‘Heterologous Expression and Nuclear localization of DNA- Binding Proteins in Human and Drosophila cells’. During this project I had an opportunity to work with a rare Chromosome Binding Protein. My work involved cloning this protein in a GFP- Overexpression vector and transfecting it in human colorectal cancer cell line. Apart from cloning, to study nuclear localization of this protein Immunofluorescence assay was also performed on Drosophila cell line. In an Independent project on another DNA Binding Protein RNA Polymerase II was carried out. Inhibitor studies were performed on this protein; MTT assay and Immunofluorescence assay were performed to study effects of RNA Polymerase II inhibitors. Confocal Microscopy helped study Immunofluorescence assay. During this project, I had a rare opportunity to interact with a scientist from Sweden. This project helped me learning basic techniques including microbial techniques, PCR, Cloning, Electrophoresis, Polyacrylamide SDS Gel Electrophoresis, and Western Blot. Cell Culture techniques including Human, Mouse and Drosophila cells. Show less