Experience

Academy of Sciences of the Czech Republic

• Czechia
• Research Services
• 200 - 300 Employee

• Researcher

  • Mar 2020 - Present

  Integrative Physiology of Gnotobionts Integrative Physiology of Gnotobionts



University of South Bohemia in České Budějovice

• Czechia
• Higher Education
• 200 - 300 Employee

• Doctoral Student

  • Aug 2015 - Mar 2020

  Insect Physiology Insect Physiology



Institute of Biochemistry, Biological Research Centre, Szeged, Hungary

• Research Services
• 1 - 100 Employee

• Internship

  • Sep 2014 - Jul 2015

  The elucidation of the molecular mechanism behind the nuclear import of Moesin, a cytoskeletal actin binding protein. I am using a Drosophila cell line S2R+ and handling various microscopes such as light and confocal laser scanning microscope. I am also using molecular biology techniques like Drosophila Gateway transfection, plasmid DNA purification, transformation, mutagenic PCR, RNA interference, gel electrophoresis and primer designing. The elucidation of the molecular mechanism behind the nuclear import of Moesin, a cytoskeletal actin binding protein. I am using a Drosophila cell line S2R+ and handling various microscopes such as light and confocal laser scanning microscope. I am also using molecular biology techniques like Drosophila Gateway transfection, plasmid DNA purification, transformation, mutagenic PCR, RNA interference, gel electrophoresis and primer designing.



University of Hyderabad

• India
• Higher Education
• 700 & Above Employee

• student

  • Jul 2012 - Jul 2014

  In master project i studied effect of the antioxidant Leucas aspera ethanolic extract on liver necrosis induced by bromobenzene, which is known to produce oxidative stress. I did screening of medicinal plant for their biological potential as antioxidants. I was exposed to extensive range of techniques for phytochemical screening qualitatively and quantitatively using different methods such as chemical and spectrophotometric techniques. The secondary metabolites were isolated by cold and hot soxhlet extraction methods. Removals of organic solvents were carried out under reduced pressure and water removal by lyophilisation techniques. Phytochemical screening and determination of flavonoids and phenolic were done using spectrophotometer. Antioxidant evaluation was carried out by in vitro using spectrophotometer and in vivo on rodents (Wistar Rats) using Bromobenzene, as inducing agents followed by histopathological and biochemical analysis. The results is indicates that methanolic extract of Leucas aspera showed hepatoprotective abilities against bromobenzene induced liver necrosis. Therefore Leucas aspera is efficient in protecting against hepatotoxicity. Show less