Marie Skłodowska-Curie Research Fellow

• Oct 2021 - Present

Sweetnam Research Fellow in Biophysics

• Oct 2020 - Oct 2021

Junior Researcher

• Jan 2017 - Sep 2020

The main object of my research was a complex process of amyloid aggregation that is related to the onset and progression of more than 50 human disorders including Alzheimer's, Parkinson's, and infective prion diseases. Main responsibilities:• Performed protein expression and purification using various chromatography methods.• Performed protein characterisation (Determination of purity, melting temperature (Tm), secondary structure, oligomerisation state etc. ).• Conceived and conducted scientific experiments.• Recorded, analysed and interpreted experimental data.• Presented results during group meetings and personally to the direct supervisor. • Organised group seminars.• Supervised undergraduate and master students. • Prepared images and figures for publications.• Contributed to writing scientific publications.• Carried out routine laboratory maintenance activities (maintained consumables inventory, carried out laboratory equipment maintenance, calibration and cleaning, kept an eye on cleanliness in the laboratory). Show less

PhD Student

• Oct 2015 - Mar 2020

The main object of my PhD research project is a complex process of amyloid aggregation, which is related to the onset and progression of multiple human disorders including Alzheimer's, Parkinson's, and infective prion diseases. Main research activities:• Conceptualisation and conduction of scientific experiments.• Cloning, recombinant protein (Amyloid-beta, tau, prion protein) expression and purification.• Characterisation of purified protein.• Monitoring of amyloid aggregation kinetics.• Mechanistic studies of amyloid fibril formation and self-replication. • Sample preparation and analysis via AFM.• Sample preparation and analysis via FTIR.• Sample analysis via DLS.• Assessment of the effects of various factors (e.g. pH, temperature, agitation, the concentration of denaturant, concentration of protein) on amyloid aggregation kinetics, aggregate morphology and secondary structure.• Assessment of effects of the molecule of interest on amyloid aggregation kinetics, aggregate morphology and secondary structure. • Writing of scientific publications. Show less

Researcher Biologist

• Feb 2015 - Feb 2018

Main responsibilities:• Performed protein expression and purification using various chromatography methods.• Performed protein characterisation (Determination of purity, melting temperature (Tm), secondary structure, oligomerisation state etc. ).• Conceived and conducted scientific experiments.• Recorded, analysed and interpreted experimental data.• Presented results during group meetings and personally to the direct supervisor. • Organised group seminars.• Supervised undergraduate students. • Prepared images and figures for publications.• Contributed to writing scientific publications.• Carried out routine laboratory maintenance activities (maintained consumables inventory, carried out laboratory equipment maintenance, calibration and cleaning, kept an eye on cleanliness in the laboratory). Show less

Laboratory Assistant

• Oct 2013 - Sep 2015

Main responsibilities:• Performed protein expression and purification using various chromatography methods.• Performed protein characterisation (Determination of purity, melting temperature (Tm), secondary structure, oligomerisation state etc. ).• Conducted and supported scientific investigations and experiments.• Planned, set up and undertook controlled experiments.• Recorded and analysed experimental data.• Presented results during group meetings and personally to the direct supervisor. • Carried out routine laboratory maintenance activities (maintained consumables inventory, carried out laboratory equipment maintenance, kept an eye on cleanliness in the laboratory). Show less

Internship at the Centre for Misfolding Disease in Prof. Michele Vendruscolo group

• Jun 2018 - Feb 2019

Main research activity: • Analysed the heterogeneity and relative abundance of Aβ42 aggregates during amyloid fibril formation reaction via high-resolution atomic force microscopy in combination with microfluidic spray deposition platform. Main responsibilities: • Performed AFM imaging. • Performed image and data processing, and further statistical analysis. • Data interpretation. • Presented results during group meetings and personally to the direct supervisor. • Prepared images and figures for publications. • Contributed to writing scientific publications. Show less

Trainee

• Sep 2011 - May 2012

Student research project: • Determination of glucose concentration in the samples using an electrochemical amperometric enzymatic sensor. Student research project: • Determination of glucose concentration in the samples using an electrochemical amperometric enzymatic sensor.