Tien Yin Yau

Senior Scientist at Everest Detection
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Contact Information
Location
San Francisco, California, United States, US
Languages
  • Spanish Elementary proficiency
  • English Native or bilingual proficiency

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Bio

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Experience

    • Research
    • 1 - 100 Employee
    • Senior Scientist
      • Apr 2019 - Present

      o Designed experiments to identify non-invasive molecular biomarkers in early cancer. o Built database to capture, track, and associate clinical data, experimental design parameters and analytical results. o Isolated nucleic acids from clinical biosamples, built sequencing libraries, analyzed NGS sequencing results using Excel, python scripts, and altair graphs. o Built interactive graphs to allow for data exploration and investigation using jupyter notebooks, Altair, and ipywidgets o Co-ordinated with CROs. Show less

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Senior Scientist
      • Jan 2019 - Apr 2019

      o Designed, validated, and optimized assays for our diagnostic blood tests (qPCR, NGS, single-cell analysis, microarray). o Piloted novel analysis techniques, interpreted data, and presented to the team. o Designed, validated, and optimized assays for our diagnostic blood tests (qPCR, NGS, single-cell analysis, microarray). o Piloted novel analysis techniques, interpreted data, and presented to the team.

    • Scientist II
      • Sep 2017 - Jan 2019

      o Piloted implementation of 10x single cell RNA-seq technology at Abbvie-Stemcentrx. Prepared all sequencing libraries.o Investigated the feasibility of using CITE-Seq to determine paired protein and transcriptome expression at the single-cell level.

    • Scientist I
      • Jun 2016 - Sep 2017

      o Wrote and maintained Python scripts that automated sample tracking and identified overlooked samples ready for different stages of processingo Validated NGS targets with qPCR (quantstudio and Biomark)

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Senior Research Associate
      • Mar 2015 - Jun 2016

      o Isolated nucleic acids from patient derived xenografts, determined expression patterns by RNA-Seq and Microarray, and analyzed results with excel and Python (pandas). o Isolated nucleic acids from patient derived xenografts, determined expression patterns by RNA-Seq and Microarray, and analyzed results with excel and Python (pandas).

    • Universty Assistant (Researcher)
      • Feb 2011 - Dec 2014

      o Designed and produced transgenic mice using ES cells, lentiviral and transposon vectors, and pronuclear injection. o Analyzed transgenic mice with fluorescence microscopy, in-vivo imaging, PCR genotyping, qPCR, and Southern/Northern/Western blots. o Designed and produced transgenic mice using ES cells, lentiviral and transposon vectors, and pronuclear injection. o Analyzed transgenic mice with fluorescence microscopy, in-vivo imaging, PCR genotyping, qPCR, and Southern/Northern/Western blots.

    • Research Services
    • 1 - 100 Employee
    • Masters Student
      • Sep 2006 - Feb 2010

      o Studied the role of the phosphatidylinositol-3-kinase pathway in disease using cancer cell lines, mammalian cell transfection, and murine models.o Determined the effects of small molecule drugs on these diseases.

    • Laboratory Assistant
      • Sep 2004 - Aug 2006

      o Produced and purified transgenic protein using bacterial and mammalian cell transfection (Transient and Lentiviral).o Determined the effect of a novel small molecule drug on inhibiting progression of hematopoietic cancers.

    • Biotechnology
    • 1 - 100 Employee
    • Laboratory Assistant
      • May 2004 - Aug 2004

      o Worked independently to clone, express, and characterize a novel protein for in house use o Constructed vectors, transformed bacteria, analyzed protein expression and purified protein. o Worked independently to clone, express, and characterize a novel protein for in house use o Constructed vectors, transformed bacteria, analyzed protein expression and purified protein.

    • Laboratory Assistant
      • Jan 2003 - Aug 2003

      o Isolated and cultivated fungi from wood samples; DNA isolation, amplification, purification, cloning, sequencing, and sequence analysis. o Developed an electronic fungal database: Organized and led database meetings. o Isolated and cultivated fungi from wood samples; DNA isolation, amplification, purification, cloning, sequencing, and sequence analysis. o Developed an electronic fungal database: Organized and led database meetings.

    • Laboratory Assistant
      • May 2002 - Aug 2002

      o Identified mites using a dissection microscope; data entry & analysis o Identified mites using a dissection microscope; data entry & analysis

    • Undergraduate Academic Assistant
      • May 2002 - Aug 2002

      o Analyzed client protein samples using a MALDI-TOF mass spectrometer o Sequenced client DNA and protein samples using automated sequencers o Analyzed client protein samples using a MALDI-TOF mass spectrometer o Sequenced client DNA and protein samples using automated sequencers

    • Laboratory Assistant
      • Nov 2000 - Aug 2001

      o In vitro Tissue Culture: Micropropagation, Meristerm culture, virus elimination and Cryopreservation o Potato DNA extraction and fingerprinting by microsatellite marker o In vitro Tissue Culture: Micropropagation, Meristerm culture, virus elimination and Cryopreservation o Potato DNA extraction and fingerprinting by microsatellite marker

    • Laboratory Assistant
      • Oct 2000 - Mar 2001

      o Performed micropropagation in orchid, pineapple, banana, African violets, and meristem culture in banana. o Performed micropropagation in orchid, pineapple, banana, African violets, and meristem culture in banana.

    • Laboratory Assistant
      • May 2000 - Jun 2000

      o Investigate the effect of habitat on behavior of Julidochromis marlieri. o Investigate the effect of habitat on behavior of Julidochromis marlieri.

Education

  • The University of British Columbia / UBC
    Master of Science (M.Sc.), Genetics
    2006 - 2010
  • The University of British Columbia / UBC
    Bachelor of Science (B.Sc.), Cell Biology and Genetics
    1999 - 2006

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