Experience

Kirk Kerkorian School of Medicine at UNLV

• United States
• Higher Education
• 100 - 200 Employee

• Assistant Professor

  • Jul 2020 - Present



University of Houston

• United States
• Higher Education
• 700 & Above Employee

• Instructional Assistant Professor

  • Sep 2011 - Aug 2019



The McGovern Medical School at the University of Texas Health Science Center at Houston

• Houston, Texas Area

• Assistant Professor

  • Sep 1993 - Aug 2008

  • Founded an independent research laboratory • Obtained $1.9 million in grant funds from the AHA and the NIH • Studied energy-dependent localization of proteins • Generated a novel 2-fluor cell sorting system that uses pseudo-FRET to isolate yeast mutants • Developed an elegant cell lysis procedure to reconstitute protein transport between two organelles in a cell-free system • Trained and supervised technical personnel • Directed >40 graduate, medical, or undergraduate… Show more • Founded an independent research laboratory • Obtained $1.9 million in grant funds from the AHA and the NIH • Studied energy-dependent localization of proteins • Generated a novel 2-fluor cell sorting system that uses pseudo-FRET to isolate yeast mutants • Developed an elegant cell lysis procedure to reconstitute protein transport between two organelles in a cell-free system • Trained and supervised technical personnel • Directed >40 graduate, medical, or undergraduate students to do experiments in biochemistry, cell biology, genetics, and molecular biology • Served and advised on >30 doctoral graduate student thesis committees • Invited >20 times to present research seminars across the US • Directed departmental Summer Undergraduate Research Program • Lectured on the pharmacology of antibiotics to med students (~200 per class) • Taught advanced microbiology, biochemistry, cell biology, and digital image analysis to grad students (~15 per class) Show less • Founded an independent research laboratory • Obtained $1.9 million in grant funds from the AHA and the NIH • Studied energy-dependent localization of proteins • Generated a novel 2-fluor cell sorting system that uses pseudo-FRET to isolate yeast mutants • Developed an elegant cell lysis procedure to reconstitute protein transport between two organelles in a cell-free system • Trained and supervised technical personnel • Directed >40 graduate, medical, or undergraduate… Show more • Founded an independent research laboratory • Obtained $1.9 million in grant funds from the AHA and the NIH • Studied energy-dependent localization of proteins • Generated a novel 2-fluor cell sorting system that uses pseudo-FRET to isolate yeast mutants • Developed an elegant cell lysis procedure to reconstitute protein transport between two organelles in a cell-free system • Trained and supervised technical personnel • Directed >40 graduate, medical, or undergraduate students to do experiments in biochemistry, cell biology, genetics, and molecular biology • Served and advised on >30 doctoral graduate student thesis committees • Invited >20 times to present research seminars across the US • Directed departmental Summer Undergraduate Research Program • Lectured on the pharmacology of antibiotics to med students (~200 per class) • Taught advanced microbiology, biochemistry, cell biology, and digital image analysis to grad students (~15 per class) Show less



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Senior Cell Biologist

  • Oct 1991 - Sep 1993

  Continuation of my research from Caltech. Through my developments in subcellular fractionation, I discovered a new organelle, the prevacuolar compartment in yeast. I also pioneered the use of a novel vital dye, FM4-64, to explore endocytosis, which is now widely used in the fields of endocytic membrane traffic, bacterial cell division, and the cytoskeleton. Continuation of my research from Caltech. Through my developments in subcellular fractionation, I discovered a new organelle, the prevacuolar compartment in yeast. I also pioneered the use of a novel vital dye, FM4-64, to explore endocytosis, which is now widely used in the fields of endocytic membrane traffic, bacterial cell division, and the cytoskeleton.



Caltech

• United States
• Research Services
• 700 & Above Employee

• Senior Research Fellow

  • Apr 1987 - Oct 1991

  I developed novel methods to permeabilize cells and maintain cellular functions. I used these approaches to reconstitute inter-compartmental transport between organelles in permeabilized yeast cells. I also designed and implemented new methods for subcellular fractionation. For these studies, I competitively obtained a junior and senior fellowship from the American Cancer Society. I developed novel methods to permeabilize cells and maintain cellular functions. I used these approaches to reconstitute inter-compartmental transport between organelles in permeabilized yeast cells. I also designed and implemented new methods for subcellular fractionation. For these studies, I competitively obtained a junior and senior fellowship from the American Cancer Society.



UT Southwestern Medical Center

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Graduate Research Assistant

  • Feb 1982 - Mar 1987

  I purified and characterized multiple forms of neprilysin, an integral plasma membrane endopeptidase that regulates activity of >10 neuronal and hormonal peptides, including β amyloid peptides. This work involved solubilizing membranes with nonionic detergents and extensive chromatography techniques. I developed immuno-assay and immuno-affinity procedures to detect (ng levels) and purify neprilysin iso-forms in mg quantities respectively. I purified and characterized multiple forms of neprilysin, an integral plasma membrane endopeptidase that regulates activity of >10 neuronal and hormonal peptides, including β amyloid peptides. This work involved solubilizing membranes with nonionic detergents and extensive chromatography techniques. I developed immuno-assay and immuno-affinity procedures to detect (ng levels) and purify neprilysin iso-forms in mg quantities respectively.



University of Florida

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Technician

  • Nov 1980 - Dec 1981