Thidar Aye
Scientist II at UL Solutions- Claim this Profile
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Bio
Credentials
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Lean Six Sigma Yellow Belt
UL SolutionsJun, 2023- Nov, 2024
Experience
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UL Solutions
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United States
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International Trade and Development
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700 & Above Employee
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Scientist II
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Feb 2023 - Present
-Lead biocompatibility tests encompassing in vitro cytotoxicity, irritation, and sensitization for medical devices, ensuring adherence to ISO 10993-1, ISO 10993-5, ISO 10993-10, ISO 10993-12, and ISO 10993-23. -Develop and research innovative test techniques, resulting in improved efficiency and accuracy. -Authored comprehensive standard operating procedures for the laboratory, ensuring consistency and quality. -Conduct thoroough document reviews to ensure regulatory compliance… Show more -Lead biocompatibility tests encompassing in vitro cytotoxicity, irritation, and sensitization for medical devices, ensuring adherence to ISO 10993-1, ISO 10993-5, ISO 10993-10, ISO 10993-12, and ISO 10993-23. -Develop and research innovative test techniques, resulting in improved efficiency and accuracy. -Authored comprehensive standard operating procedures for the laboratory, ensuring consistency and quality. -Conduct thoroough document reviews to ensure regulatory compliance according to Good Laboratory Practice (GLP). -Perform cost-analysis research for test implementation, optimizing resource application. -Spearhead scope expansion, initiation, researching, and implementing new capabilities within the laboratory. -Actively participated in an audit of the lab, ensuring adherence to quality standards and identifying areas for improvement. Show less -Lead biocompatibility tests encompassing in vitro cytotoxicity, irritation, and sensitization for medical devices, ensuring adherence to ISO 10993-1, ISO 10993-5, ISO 10993-10, ISO 10993-12, and ISO 10993-23. -Develop and research innovative test techniques, resulting in improved efficiency and accuracy. -Authored comprehensive standard operating procedures for the laboratory, ensuring consistency and quality. -Conduct thoroough document reviews to ensure regulatory compliance… Show more -Lead biocompatibility tests encompassing in vitro cytotoxicity, irritation, and sensitization for medical devices, ensuring adherence to ISO 10993-1, ISO 10993-5, ISO 10993-10, ISO 10993-12, and ISO 10993-23. -Develop and research innovative test techniques, resulting in improved efficiency and accuracy. -Authored comprehensive standard operating procedures for the laboratory, ensuring consistency and quality. -Conduct thoroough document reviews to ensure regulatory compliance according to Good Laboratory Practice (GLP). -Perform cost-analysis research for test implementation, optimizing resource application. -Spearhead scope expansion, initiation, researching, and implementing new capabilities within the laboratory. -Actively participated in an audit of the lab, ensuring adherence to quality standards and identifying areas for improvement. Show less
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Research Technician
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Apr 2022 - Feb 2023
• Managed a comprehensive mice colony program, including breeding plans, genotyping, weaning, and tumor monitoring, supporting the lab's essential animal-based research. • Applied Polymerase Chain Reaction (PCR) tests and agarose gel electrophoresis for precise genotyping of mice, while also contributing to the development of an optimized PCR protocol. • Maintained multiple mammalian cell lines, employing aseptic cell culture techniques to ensure optimal cell health and experimental… Show more • Managed a comprehensive mice colony program, including breeding plans, genotyping, weaning, and tumor monitoring, supporting the lab's essential animal-based research. • Applied Polymerase Chain Reaction (PCR) tests and agarose gel electrophoresis for precise genotyping of mice, while also contributing to the development of an optimized PCR protocol. • Maintained multiple mammalian cell lines, employing aseptic cell culture techniques to ensure optimal cell health and experimental integrity. • Conducted bacterial transformation and employed genomic extraction, purification, and quantification techniques, utilizing mini- and maxi-prep protocols to ensure high-quality genetic material for analysis. • Led training sessions for new team members, imparting proper laboratory and experimental techniques to ensure consistent and effective workflow. • Represented the lab during Institutional Animal Care and Use Committee (IACUC) inspections, showcasing compliance and standards in mice research. Show less • Managed a comprehensive mice colony program, including breeding plans, genotyping, weaning, and tumor monitoring, supporting the lab's essential animal-based research. • Applied Polymerase Chain Reaction (PCR) tests and agarose gel electrophoresis for precise genotyping of mice, while also contributing to the development of an optimized PCR protocol. • Maintained multiple mammalian cell lines, employing aseptic cell culture techniques to ensure optimal cell health and experimental… Show more • Managed a comprehensive mice colony program, including breeding plans, genotyping, weaning, and tumor monitoring, supporting the lab's essential animal-based research. • Applied Polymerase Chain Reaction (PCR) tests and agarose gel electrophoresis for precise genotyping of mice, while also contributing to the development of an optimized PCR protocol. • Maintained multiple mammalian cell lines, employing aseptic cell culture techniques to ensure optimal cell health and experimental integrity. • Conducted bacterial transformation and employed genomic extraction, purification, and quantification techniques, utilizing mini- and maxi-prep protocols to ensure high-quality genetic material for analysis. • Led training sessions for new team members, imparting proper laboratory and experimental techniques to ensure consistent and effective workflow. • Represented the lab during Institutional Animal Care and Use Committee (IACUC) inspections, showcasing compliance and standards in mice research. Show less
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The Conlon Lab
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University of North Carolina at Chapel Hill
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Undergraduate Reserach Assistant
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May 2021 - Apr 2022
Congenital heart disease (CHD) is one of the most prevalent forms of birth defects. Various abnormalities during embryonic development are involved in its occurrence. The primary interest of our lab, the Conlon lab, is to understand the molecular events of embryonic development to inform CHD. Our lab used quantitative proteomics to identify proteins within the heart. From this, we identified one pathway of interest, the mevalonate pathway (MVA), which takes Acetoacetyl-CoA to performs… Show more Congenital heart disease (CHD) is one of the most prevalent forms of birth defects. Various abnormalities during embryonic development are involved in its occurrence. The primary interest of our lab, the Conlon lab, is to understand the molecular events of embryonic development to inform CHD. Our lab used quantitative proteomics to identify proteins within the heart. From this, we identified one pathway of interest, the mevalonate pathway (MVA), which takes Acetoacetyl-CoA to performs cholesterol synthesis and protein prenylation. MVA is involved in many important cellular functions, including cell proliferation, survival, migration, and apoptosis. However, its role in heart development is not fully understood. Our analysis of proteomics data shows that the majority of proteins within this pathway are highly expressed during critical time points in development. Therefore, we hypothesized that this pathway may regulate proteins responsible for CHD, specifically those involved in cellular signaling and proliferation in embryonic cardiomyocytes. To assess the role of MVA in the developing heart, I performed fluorescence IHC to locate expressions of GGPS1 and HMGCR, two critical proteins in MVA, at critical stages of embryonic development (E 9.5, E12.5, and E16.5). We found that GGPS1 is predominantly expressed in cytoplasm of cardiomyocytes in the interventricular septum, atria, left ventricle, and right ventricle. HMGCR1 is initially highly expressed in the atria and ventricles but is specifically expressed in the endothelial cells within the major components of the heart. Our double stain analysis of GGPS1 and Tropomyosin shows that GGPS1 is expressed in the cardiocytes of the major components and trabeculae. Given that its proteins are dynamically expressed in the developing heart, we believe that increased understanding of the MVA pathway will inform developmental abnormalities that contribute to congenital heart disease. Show less Congenital heart disease (CHD) is one of the most prevalent forms of birth defects. Various abnormalities during embryonic development are involved in its occurrence. The primary interest of our lab, the Conlon lab, is to understand the molecular events of embryonic development to inform CHD. Our lab used quantitative proteomics to identify proteins within the heart. From this, we identified one pathway of interest, the mevalonate pathway (MVA), which takes Acetoacetyl-CoA to performs… Show more Congenital heart disease (CHD) is one of the most prevalent forms of birth defects. Various abnormalities during embryonic development are involved in its occurrence. The primary interest of our lab, the Conlon lab, is to understand the molecular events of embryonic development to inform CHD. Our lab used quantitative proteomics to identify proteins within the heart. From this, we identified one pathway of interest, the mevalonate pathway (MVA), which takes Acetoacetyl-CoA to performs cholesterol synthesis and protein prenylation. MVA is involved in many important cellular functions, including cell proliferation, survival, migration, and apoptosis. However, its role in heart development is not fully understood. Our analysis of proteomics data shows that the majority of proteins within this pathway are highly expressed during critical time points in development. Therefore, we hypothesized that this pathway may regulate proteins responsible for CHD, specifically those involved in cellular signaling and proliferation in embryonic cardiomyocytes. To assess the role of MVA in the developing heart, I performed fluorescence IHC to locate expressions of GGPS1 and HMGCR, two critical proteins in MVA, at critical stages of embryonic development (E 9.5, E12.5, and E16.5). We found that GGPS1 is predominantly expressed in cytoplasm of cardiomyocytes in the interventricular septum, atria, left ventricle, and right ventricle. HMGCR1 is initially highly expressed in the atria and ventricles but is specifically expressed in the endothelial cells within the major components of the heart. Our double stain analysis of GGPS1 and Tropomyosin shows that GGPS1 is expressed in the cardiocytes of the major components and trabeculae. Given that its proteins are dynamically expressed in the developing heart, we believe that increased understanding of the MVA pathway will inform developmental abnormalities that contribute to congenital heart disease. Show less
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Education
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2018 - 2022
University of North Carolina at Chapel Hill
Bachelor of Science - BS, Biology, General
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