Tatiani Brenelli de Lima
MS Specialist at Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)- Claim this Profile
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Português Native or bilingual proficiency
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Inglês Full professional proficiency
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Bio
Valeria Scorsato
A Dra. Tatiani é uma profissional de excelência! Eu tive o prazer de conhece-la durante nossas pesquisas de doutorado. Ela me auxiliou na minha pesquisa sobre estrutura de proteínas com as técnicas de MS. E juntas tivemos resultados de sucesso. Ela domina as técnicas que envolvem Espectrometria de Massas (LC-MS, LC_MS/MS, cross-linking, HDX por exemplo). Apesar de ser formada em química, sua visão e conhecimentos sobre ciências é multidisciplinar. Sua inteligência emocional, autocontrole e antifragilidade são muito elevadas, além disso a Tatiani é muito responsável e criativa.
Valeria Scorsato
A Dra. Tatiani é uma profissional de excelência! Eu tive o prazer de conhece-la durante nossas pesquisas de doutorado. Ela me auxiliou na minha pesquisa sobre estrutura de proteínas com as técnicas de MS. E juntas tivemos resultados de sucesso. Ela domina as técnicas que envolvem Espectrometria de Massas (LC-MS, LC_MS/MS, cross-linking, HDX por exemplo). Apesar de ser formada em química, sua visão e conhecimentos sobre ciências é multidisciplinar. Sua inteligência emocional, autocontrole e antifragilidade são muito elevadas, além disso a Tatiani é muito responsável e criativa.
Valeria Scorsato
A Dra. Tatiani é uma profissional de excelência! Eu tive o prazer de conhece-la durante nossas pesquisas de doutorado. Ela me auxiliou na minha pesquisa sobre estrutura de proteínas com as técnicas de MS. E juntas tivemos resultados de sucesso. Ela domina as técnicas que envolvem Espectrometria de Massas (LC-MS, LC_MS/MS, cross-linking, HDX por exemplo). Apesar de ser formada em química, sua visão e conhecimentos sobre ciências é multidisciplinar. Sua inteligência emocional, autocontrole e antifragilidade são muito elevadas, além disso a Tatiani é muito responsável e criativa.
Valeria Scorsato
A Dra. Tatiani é uma profissional de excelência! Eu tive o prazer de conhece-la durante nossas pesquisas de doutorado. Ela me auxiliou na minha pesquisa sobre estrutura de proteínas com as técnicas de MS. E juntas tivemos resultados de sucesso. Ela domina as técnicas que envolvem Espectrometria de Massas (LC-MS, LC_MS/MS, cross-linking, HDX por exemplo). Apesar de ser formada em química, sua visão e conhecimentos sobre ciências é multidisciplinar. Sua inteligência emocional, autocontrole e antifragilidade são muito elevadas, além disso a Tatiani é muito responsável e criativa.
Experience
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Brazilian Center for Research in Energy and Materials (CNPEM)
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Brazil
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Research Services
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700 & Above Employee
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MS Specialist
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Nov 2020 - Present
- Metabolomics Facility Manager; - Development, optimization and implementation of methodologies to analyze metabolites (lipids, carbohydrates, glycan, primary and secondary metabolites) and biomass derivatives by MALDI-MS, LC-MS and LC-MS/MS; - Development of MS-based approaches for enzymatic studies, proteomics and structural proteomics; - Hands-on experience in MS (Synapt XS and LTQ-XL); - Internship supervisor. - Metabolomics Facility Manager; - Development, optimization and implementation of methodologies to analyze metabolites (lipids, carbohydrates, glycan, primary and secondary metabolites) and biomass derivatives by MALDI-MS, LC-MS and LC-MS/MS; - Development of MS-based approaches for enzymatic studies, proteomics and structural proteomics; - Hands-on experience in MS (Synapt XS and LTQ-XL); - Internship supervisor.
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Escola Técnica Estadual "Conselheiro Antonio Prado"
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Brazil
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Biotechnology Research
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1 - 100 Employee
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Secondary Technical Education Teacher
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Aug 2022 - Aug 2023
Subjects: - Good laboratory practices (GLP) - Instrumental chemistry analysis - Biochemistry - Analytical chemistry - Qualitative analytical chemistry - Capstone project planning Subjects: - Good laboratory practices (GLP) - Instrumental chemistry analysis - Biochemistry - Analytical chemistry - Qualitative analytical chemistry - Capstone project planning
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Goethe University Frankfurt
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Germany
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Higher Education
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700 & Above Employee
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Postdoctoral Research Fellow
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Feb 2019 - Jan 2020
- Clinical proteomics by LC-MS/MS (quantitative proteomics and phosphoproteomics of acute myeloid leukemia and lymphomas) - Proteomics data analysis (R studio, MaxQuant, Perseus) - Clinical proteomics by LC-MS/MS (quantitative proteomics and phosphoproteomics of acute myeloid leukemia and lymphomas) - Proteomics data analysis (R studio, MaxQuant, Perseus)
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Solvay
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Belgium
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Chemical Manufacturing
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700 & Above Employee
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Research Scientist - Inova Talentos
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Aug 2018 - Jan 2019
- Development, optimization and implementation of methodologies to analyse surfactants, biosurfactants and biomass derivatives by LC-MS and LC/MS/MS - Agilent Q-TOF 6530-C operation and maintenance - Development, optimization and implementation of methodologies to analyse surfactants, biosurfactants and biomass derivatives by LC-MS and LC/MS/MS - Agilent Q-TOF 6530-C operation and maintenance
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Laboratório Nacional de Biociências (LNBio)
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Research
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1 - 100 Employee
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PHD Candidate
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Feb 2016 - Mar 2018
- Cell culture (HEK293T) and cell transfection - Characterization of proteins and protein-protein interactions by SDS-PAGE, western blot and co-immunoprecipitation - Plasmid amplification by E.coli transformation and midi-prep - Protein expression and purification - Cell culture (HEK293T) and cell transfection - Characterization of proteins and protein-protein interactions by SDS-PAGE, western blot and co-immunoprecipitation - Plasmid amplification by E.coli transformation and midi-prep - Protein expression and purification
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State University of Campinas
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Research Services
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100 - 200 Employee
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PHD Candidate
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Mar 2014 - Mar 2018
- Structural proteomics by LC-MS (chemical cross-linking, hydrogen-deuterium exchange and native MS coupled to mass spectrometry)- Shotgun proteomics (identification of proteins and post-translational modifications)- UPLC, Synapt G1-HDMS and Xevo Q-TOF operation and maintenance- Q-Exactive operation- Teaching internship: Organic Chemistry (theorical and experimental) and biochemistry
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Master Candidate
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Feb 2012 - Feb 2014
- Structural proteomics by LC-MS (chemical cross-linking and hydrogen-deuterium exchange coupled to mass spectrometry)- Shotgun proteomics (identification of proteins and post-translational modifications)- UPLC, Synapt G1-HDMS and Xevo Q-TOF operation and maintenance- Protein structure prediction (I-tasser)
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Marthin Luther Halle-Wittenberg University
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Halle-Neustadt e Região, Alemanha
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Visiting Research Fellow
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Nov 2017 - Jan 2018
I went to the Sinz lab to characterize the three-dimensional structure of the ultrastable p53 complex using a new MS-cleavable cross-linker - Protein expression and purification - Structural proteomics by MS (chemical cross-linking and native MS) I went to the Sinz lab to characterize the three-dimensional structure of the ultrastable p53 complex using a new MS-cleavable cross-linker - Protein expression and purification - Structural proteomics by MS (chemical cross-linking and native MS)
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CLS Tecnologia Analítica e Sistemas de Gestão Ltda
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Brazil
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Personal Care Product Manufacturing
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1 - 100 Employee
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Internship
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Jul 2011 - Dec 2011
- Analysis of chemical products by liquid and gas chromatography - Analysis of chemical products by liquid and gas chromatography
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Pontifícia Universidade Católica de Campinas
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Brazil
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Higher Education
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700 & Above Employee
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Research Internship
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Oct 2008 - Jun 2011
- Synthesis and biosynthesis of biosurfactant; - Cyanobacteria inhibitions assays; - ELISA assays; - UV-vis spectroscopy analysis; - Cyanobacterias cell culture; - Analysis of microcystins by liquid chromatography; - Analysis of compounds by gas chromatography and atomic absorption spectroscopy; - Teaching internship: Organic Chemistry, Inorganic Chemistry and Biochemistry - Synthesis and biosynthesis of biosurfactant; - Cyanobacteria inhibitions assays; - ELISA assays; - UV-vis spectroscopy analysis; - Cyanobacterias cell culture; - Analysis of microcystins by liquid chromatography; - Analysis of compounds by gas chromatography and atomic absorption spectroscopy; - Teaching internship: Organic Chemistry, Inorganic Chemistry and Biochemistry
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Education
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Universidade Estadual de Campinas
Doctor of Philosophy - PhD, Organic Chemistry and Biochemistry -
Universidade Estadual de Campinas
Master's degree, Organic Chemistry -
Pontifícia Universidade Católica de Campinas
Química Tecnológica -
Escola Técnica Estadual Conselheiro Antonio Prado
Técnico em Bioquímica