Tatiani Brenelli de Lima

MS Specialist at Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)
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Contact Information
us****@****om
(386) 825-5501
Location
Campinas, São Paulo, Brazil, BR
Languages
  • Português Native or bilingual proficiency
  • Inglês Full professional proficiency

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Valeria Scorsato

A Dra. Tatiani é uma profissional de excelência! Eu tive o prazer de conhece-la durante nossas pesquisas de doutorado. Ela me auxiliou na minha pesquisa sobre estrutura de proteínas com as técnicas de MS. E juntas tivemos resultados de sucesso. Ela domina as técnicas que envolvem Espectrometria de Massas (LC-MS, LC_MS/MS, cross-linking, HDX por exemplo). Apesar de ser formada em química, sua visão e conhecimentos sobre ciências é multidisciplinar. Sua inteligência emocional, autocontrole e antifragilidade são muito elevadas, além disso a Tatiani é muito responsável e criativa.

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Experience

    • Brazil
    • Research Services
    • 700 & Above Employee
    • MS Specialist
      • Nov 2020 - Present

      - Metabolomics Facility Manager; - Development, optimization and implementation of methodologies to analyze metabolites (lipids, carbohydrates, glycan, primary and secondary metabolites) and biomass derivatives by MALDI-MS, LC-MS and LC-MS/MS; - Development of MS-based approaches for enzymatic studies, proteomics and structural proteomics; - Hands-on experience in MS (Synapt XS and LTQ-XL); - Internship supervisor. - Metabolomics Facility Manager; - Development, optimization and implementation of methodologies to analyze metabolites (lipids, carbohydrates, glycan, primary and secondary metabolites) and biomass derivatives by MALDI-MS, LC-MS and LC-MS/MS; - Development of MS-based approaches for enzymatic studies, proteomics and structural proteomics; - Hands-on experience in MS (Synapt XS and LTQ-XL); - Internship supervisor.

    • Brazil
    • Biotechnology Research
    • 1 - 100 Employee
    • Secondary Technical Education Teacher
      • Aug 2022 - Aug 2023

      Subjects: - Good laboratory practices (GLP) - Instrumental chemistry analysis - Biochemistry - Analytical chemistry - Qualitative analytical chemistry - Capstone project planning Subjects: - Good laboratory practices (GLP) - Instrumental chemistry analysis - Biochemistry - Analytical chemistry - Qualitative analytical chemistry - Capstone project planning

    • Germany
    • Higher Education
    • 700 & Above Employee
    • Postdoctoral Research Fellow
      • Feb 2019 - Jan 2020

      - Clinical proteomics by LC-MS/MS (quantitative proteomics and phosphoproteomics of acute myeloid leukemia and lymphomas) - Proteomics data analysis (R studio, MaxQuant, Perseus) - Clinical proteomics by LC-MS/MS (quantitative proteomics and phosphoproteomics of acute myeloid leukemia and lymphomas) - Proteomics data analysis (R studio, MaxQuant, Perseus)

    • Belgium
    • Chemical Manufacturing
    • 700 & Above Employee
    • Research Scientist - Inova Talentos
      • Aug 2018 - Jan 2019

      - Development, optimization and implementation of methodologies to analyse surfactants, biosurfactants and biomass derivatives by LC-MS and LC/MS/MS - Agilent Q-TOF 6530-C operation and maintenance - Development, optimization and implementation of methodologies to analyse surfactants, biosurfactants and biomass derivatives by LC-MS and LC/MS/MS - Agilent Q-TOF 6530-C operation and maintenance

    • PHD Candidate
      • Feb 2016 - Mar 2018

      - Cell culture (HEK293T) and cell transfection - Characterization of proteins and protein-protein interactions by SDS-PAGE, western blot and co-immunoprecipitation - Plasmid amplification by E.coli transformation and midi-prep - Protein expression and purification - Cell culture (HEK293T) and cell transfection - Characterization of proteins and protein-protein interactions by SDS-PAGE, western blot and co-immunoprecipitation - Plasmid amplification by E.coli transformation and midi-prep - Protein expression and purification

    • Research Services
    • 100 - 200 Employee
    • PHD Candidate
      • Mar 2014 - Mar 2018

      - Structural proteomics by LC-MS (chemical cross-linking, hydrogen-deuterium exchange and native MS coupled to mass spectrometry)- Shotgun proteomics (identification of proteins and post-translational modifications)- UPLC, Synapt G1-HDMS and Xevo Q-TOF operation and maintenance- Q-Exactive operation- Teaching internship: Organic Chemistry (theorical and experimental) and biochemistry

    • Master Candidate
      • Feb 2012 - Feb 2014

      - Structural proteomics by LC-MS (chemical cross-linking and hydrogen-deuterium exchange coupled to mass spectrometry)- Shotgun proteomics (identification of proteins and post-translational modifications)- UPLC, Synapt G1-HDMS and Xevo Q-TOF operation and maintenance- Protein structure prediction (I-tasser)

  • Marthin Luther Halle-Wittenberg University
    • Halle-Neustadt e Região, Alemanha
    • Visiting Research Fellow
      • Nov 2017 - Jan 2018

      I went to the Sinz lab to characterize the three-dimensional structure of the ultrastable p53 complex using a new MS-cleavable cross-linker - Protein expression and purification - Structural proteomics by MS (chemical cross-linking and native MS) I went to the Sinz lab to characterize the three-dimensional structure of the ultrastable p53 complex using a new MS-cleavable cross-linker - Protein expression and purification - Structural proteomics by MS (chemical cross-linking and native MS)

    • Brazil
    • Personal Care Product Manufacturing
    • 1 - 100 Employee
    • Internship
      • Jul 2011 - Dec 2011

      - Analysis of chemical products by liquid and gas chromatography - Analysis of chemical products by liquid and gas chromatography

    • Brazil
    • Higher Education
    • 700 & Above Employee
    • Research Internship
      • Oct 2008 - Jun 2011

      - Synthesis and biosynthesis of biosurfactant; - Cyanobacteria inhibitions assays; - ELISA assays; - UV-vis spectroscopy analysis; - Cyanobacterias cell culture; - Analysis of microcystins by liquid chromatography; - Analysis of compounds by gas chromatography and atomic absorption spectroscopy; - Teaching internship: Organic Chemistry, Inorganic Chemistry and Biochemistry - Synthesis and biosynthesis of biosurfactant; - Cyanobacteria inhibitions assays; - ELISA assays; - UV-vis spectroscopy analysis; - Cyanobacterias cell culture; - Analysis of microcystins by liquid chromatography; - Analysis of compounds by gas chromatography and atomic absorption spectroscopy; - Teaching internship: Organic Chemistry, Inorganic Chemistry and Biochemistry

Education

  • Universidade Estadual de Campinas
    Doctor of Philosophy - PhD, Organic Chemistry and Biochemistry
    2014 - 2018
  • Universidade Estadual de Campinas
    Master's degree, Organic Chemistry
    2012 - 2014
  • Pontifícia Universidade Católica de Campinas
    Química Tecnológica
    2008 - 2012
  • Escola Técnica Estadual Conselheiro Antonio Prado
    Técnico em Bioquímica
    2006 - 2007

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