Tapfuma Mutukura

Senior Research Associate at BlueRock Therapeutics
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Contact Information
us****@****om
(386) 825-5501
Location
Brampton, Ontario, Canada, CA

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Experience

    • United States
    • Biotechnology Research
    • 300 - 400 Employee
    • Senior Research Associate
      • Mar 2020 - Present

      iPSC cell culture IPSC cell differentiation into macrophages for downstream functional assays Optimization of the iPSC to macrophage differentiation process Polarization and phenotyping of macrophages derived from iPSC Phagocytosis assays with immune cells derived from iPSC iPSC cell culture IPSC cell differentiation into macrophages for downstream functional assays Optimization of the iPSC to macrophage differentiation process Polarization and phenotyping of macrophages derived from iPSC Phagocytosis assays with immune cells derived from iPSC

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Research Associate II
      • Mar 2015 - Oct 2019

      • Primary cell isolation from whole-blood and subsequent culture and analysis • Tumor cell culture and analysis • Multicolor flow cytometry • Tumor dissociation and cell analysis • Immune cell killing assay • Phagocytosis and efferocytosis assays • Multiplexed immunoassays • siRNA gene knockdown • ELISA • Western Blotting • Apoptosis assays • Primary cell isolation from whole-blood and subsequent culture and analysis • Tumor cell culture and analysis • Multicolor flow cytometry • Tumor dissociation and cell analysis • Immune cell killing assay • Phagocytosis and efferocytosis assays • Multiplexed immunoassays • siRNA gene knockdown • ELISA • Western Blotting • Apoptosis assays

  • Amorfix Life Sciences Ltd.
    • Mississauga, Ontario, Canada
    • Research Associate II
      • Feb 2012 - Feb 2015

      • Cell culture for cytotoxic therapeutic conjugate development and testing • ELISA development for Amyotrophic Lateral Sclerosis diagnostic assay • Fas specific antibody screening • Routine HABA assays and WST-1 cell proliferation assays • Cell staining for Proteinase K and 1c-120-Urease activity test by flow cytometry • Involved in general lab maintenance and up-keep • Cell culture for cytotoxic therapeutic conjugate development and testing • ELISA development for Amyotrophic Lateral Sclerosis diagnostic assay • Fas specific antibody screening • Routine HABA assays and WST-1 cell proliferation assays • Cell staining for Proteinase K and 1c-120-Urease activity test by flow cytometry • Involved in general lab maintenance and up-keep

    • Canada
    • Biotechnology Research
    • 1 - 100 Employee
    • Development Associate
      • Jan 2011 - Nov 2011

      • Conducting experiments during the initial discovery stages of assay development for autoimmune diseases like rheumatoid arthritis, celiac, and vasculitis • Development of a microarray based assay for the diagnosis of autoimmune diseases • Working with the platform automation team to diagnose and rectify software and hardware issues for the automated microarray platform. • Optimizing an automated method for the diagnosis of autoimmune diseases • Verification and validation studies for research use only (RUO), investigational use only (IUO), and in-vitro diagnostics (IVD) automated microarray platforms for autoimmune diseases • Performing technology transfer from development to manufacturing Show less

    • United States
    • Research Services
    • 1 - 100 Employee
    • Clinical Study Coordinator
      • Jun 2010 - Dec 2010

      • Conducting clinical trials as per study protocol and standard operating procedures • Subject consenting, vital signs measurements, explanation of study drug administration, Spirometry, and Acoustic Rhinometry • Input of Information into source documents and cooperating with internal Quality Assurance department, Sponsors, and Monitors during monitoring visits, audits, and follow ups • Preparing study documents which include source documents, study binder, logs, and tracking forms • Conducting clinical trials as per study protocol and standard operating procedures • Subject consenting, vital signs measurements, explanation of study drug administration, Spirometry, and Acoustic Rhinometry • Input of Information into source documents and cooperating with internal Quality Assurance department, Sponsors, and Monitors during monitoring visits, audits, and follow ups • Preparing study documents which include source documents, study binder, logs, and tracking forms

  • Amorfix Life Sciences Ltd.
    • Toronto, Ontario, Canada
    • Research Associate II
      • Sep 2006 - May 2010

      • Developed and tested a plasma based prion detection assay • Coupled antibody to europium and magnetic beads for use in bead assays. • Involved in the production and quality control of diagnostic kits for prion disease detection • Optimized brain homogenate assays for human, mouse, sheep, and hamster • Involved in general lab maintenance and up-keep • Developed and tested a plasma based prion detection assay • Coupled antibody to europium and magnetic beads for use in bead assays. • Involved in the production and quality control of diagnostic kits for prion disease detection • Optimized brain homogenate assays for human, mouse, sheep, and hamster • Involved in general lab maintenance and up-keep

Education

  • University of Toronto
    Master's degree, Pharmacology
  • University of Toronto
    MSc, Pharmacology
    2003 - 2005
  • University of Zimbabwe
    BSc, Biochemistry
    1999 - 2002

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