Experience

The TIM Company
• Netherlands
• Research Services
• 1 - 100 Employee
• Project Manager

  • Aug 2021 - Present


Elsevier

• Netherlands
• IT Services and IT Consulting
• 700 & Above Employee

• Associate Editor

  • Jan 2021 - Present

  I support the Editor-in-Chief on the first assessment of new submissions, searching and inviting reviewers, monitoring submission and revision process and final publication decision. "Human Nutrition & Metabolism is dedicated to stimulating and publishing research which focuses on the physiology and pathophysiology of nutrition and metabolism in humans. The journal publishes original papers, reviews, commentaries and opinion pieces in human nutritional and metabolic science to bridge the gap between preclinical and clinical work. Papers can focus on areas such as lifestyle, diabetes, insulin resistance, metabolic diseases, undernutrition, in-hospital nutrition, disease specific nutrition, macronutrients and micronutrients. Human Nutrition & Metabolism welcomes manuscripts from pediatrics to geriatrics regardless of global background."



AMC - Academic Medical Center

• PHD Candidate

  • Jan 2016 - 2021

  Topic: The impact of early life antibiotics on gut maturation Antibiotics are the most commonly prescribed medication in pediatric care, both to prevent and treat infectious diseases. There are, however, several diseases associated with the prolonged use of early life antibiotics. I aimed to investigate the effects of early life antibiotic treatment on intestinal development after birth. Main projects: • establish, optimize and characterize an in vitro model to study intestinal postnatal maturation using mouse fetal intestinal organoids (published in EMBO Reports). • video protocol of mouse fetal intestinal organoids model (published in JOVE). • study the direct effects of antibiotics on the intestinal cells, by comparing the treatment in young mice with the treatment of mouse fetal intestinal organoids (under revision). • determine the long-term consequences of early life antibiotic treatment on the adult intestine (in preparation). • optimize and characterize the culture of human fetal intestinal organoids (to be finished by next PhD candidate). Responsibilities: • work in ISO 15189 accreditation environment. • conduct experiments using young mice, fetal and adult/mouse and human/2D and 3D intestinal organoids, qRT-PCR, immunostainings, seahorse metabolic assays, enzyme activity assays, microarray analysis, FACS and ELISA. • obtain approval for a 5-year project from the Dutch Committee for Animal Research (Aanvraag Projectvergunning Dierproeven), including concept creation, experimental planning, protocol development, and resource planning. • closely collaborate with researchers within my group and the department, and from other groups in the hospital and external partners. • present results in several national and international conferences, for both scientific and lay audiences. • train other researchers in certain techniques. • supervise 3 students in thesis and project proposal writing and presenting. • organize biweekly literature discussions.



Restaurant De Jong

• Netherlands
• Restaurants

• Dishwasher

  • Nov 2016 - Jan 2017

  The name says it all :) I needed some income while applying to PhD positions. It was a good opportunity to practice my Dutch and be in close contact with one of my passions: cooking! The name says it all :) I needed some income while applying to PhD positions. It was a good opportunity to practice my Dutch and be in close contact with one of my passions: cooking!



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Intern

  • Sep 2014 - Aug 2015

  Topic: The role of cKit receptor in the Paneth cells as the stem cell niche of the small intestine and in the Paneth-like cells of the colon (Master Thesis project) Research group of Riccardo Fodde (Pathology Department) Techniques: isolation and culture of mouse intestinal organoids, cell lines culture, organoid reconstitution assay, qRT-PCR, TOP-FOP assay, plasmid amplification, cloning. Topic: The role of cKit receptor in the Paneth cells as the stem cell niche of the small intestine and in the Paneth-like cells of the colon (Master Thesis project) Research group of Riccardo Fodde (Pathology Department) Techniques: isolation and culture of mouse intestinal organoids, cell lines culture, organoid reconstitution assay, qRT-PCR, TOP-FOP assay, plasmid amplification, cloning.

• Research Intern

  • Nov 2013 - Jul 2014

  Topic: Genetic manipulation of mouse liver organoids for production of α-L-iduronidase as a gene therapy of Hurler’s disease Research group Derk ten Berg (Cell Biology Department) Techniques: culture of mouse liver organoids, genotyping, qRT-PCR, enzyme assays, immunofluorescence, confocal microscopy. Topic: Genetic manipulation of mouse liver organoids for production of α-L-iduronidase as a gene therapy of Hurler’s disease Research group Derk ten Berg (Cell Biology Department) Techniques: culture of mouse liver organoids, genotyping, qRT-PCR, enzyme assays, immunofluorescence, confocal microscopy.



ISN

• Canada
• Insurance
• 1 - 100 Employee

• Lifeguard

  • Jul 2010 - Aug 2012

  3 summers in different locations 3 summers in different locations



Science Museum of the University of Coimbra

• Museum Assistant

  • Sep 2011 - Apr 2012

  Organize and lead activities with children (6 to 12 years old). Guide museum tours to individuals and groups. Catalog and clean museum specimens. Organize and lead activities with children (6 to 12 years old). Guide museum tours to individuals and groups. Catalog and clean museum specimens.