Experience

Institute for Molecular Medicine Finland (FIMM)

• Finland
• Research
• 1 - 100 Employee

• Laboratory Analyst (NGS Sequencing Unit)

  • May 2022 - Present



University of Helsinki

• Finland
• Higher Education
• 700 & Above Employee

• Research Laboratory Technician (Genome Stability Group Kauppi)

  • Aug 2018 - Nov 2020

  - Carried out, planned and optimized translational research to advance individualized treatment for ovarian cancer: develop diagnostic ex vivo functional assay to identify patients' DNA repair capacity and chemotherapy response and find chemosensitization approaches for treatment-resistant patients - Focused on cell culture and treatments (>200 patient tumors and commercial cell lines) and immunostaining (>1000 histological sections) - Coordinated TERVA Chemoresponse consortium… Show more - Carried out, planned and optimized translational research to advance individualized treatment for ovarian cancer: develop diagnostic ex vivo functional assay to identify patients' DNA repair capacity and chemotherapy response and find chemosensitization approaches for treatment-resistant patients - Focused on cell culture and treatments (>200 patient tumors and commercial cell lines) and immunostaining (>1000 histological sections) - Coordinated TERVA Chemoresponse consortium members and shared patient samples (TERVA=Health from Science program by Academy of Finland and Finnish Cancer Foundation) - Improved infrastructure and logistics of fresh patient sample transport from clinical collaborators in another city - Coordinated laboratory moving and setup - Responsible for consortium's social media science account and presented at science events - Organized retreats for the consortium

• Project Coordinator (Battersby Lab)

  • Feb 2012 - Aug 2018

  - Carried out, planned and optimized basic research to study regulation of mitochondrial gene expression in an international research group, part of FinMIT (an Academy of Finland Centre of Excellence) - Focused on genotyping, Gateway cloning, protein work and cell culture - Coordinated breeding and maintenance of mouse colonies with Laboratory Animal Centre - Maintained laboratory organized, safe and well-supplied, wrote protocols - Coordinated laboratory moving and setup -… Show more - Carried out, planned and optimized basic research to study regulation of mitochondrial gene expression in an international research group, part of FinMIT (an Academy of Finland Centre of Excellence) - Focused on genotyping, Gateway cloning, protein work and cell culture - Coordinated breeding and maintenance of mouse colonies with Laboratory Animal Centre - Maintained laboratory organized, safe and well-supplied, wrote protocols - Coordinated laboratory moving and setup - Trained undergraduate students and co-workers Learned: - Cell culture (siRNA and plasmid transfection, transgene overexpression via retroviral transduction and transient transfection, immortalization) - DNA-work (extraction, Gateway cloning, site-directed mutagenesis, gene SOEing, Advanced Analytical fragment analyzer) - RNA-work (extraction, Agilent Bioanalyzer 2100 capillary electrophoresis) - Protein work (extraction, SDS-PAGE, blue native, sucrose gradient separation, sodium carbonate extraction of membrane proteins) - Mouse work (tissue and embryo collection, genotyping, primary cell line generation) - 35S radioactive work - FACS

• Master Student (Battersby Lab)

  • Mar 2010 - Feb 2012

  - Completed a MSc thesis project in an international research group, part of FinMIT (an Academy of Finland Centre of Excellence) - Presented results to local science community Thesis title: Establishing transgenic mouse lines for studying mitochondrial DNA segregation and analyzing Gimap3 allelic expression



Yhtyneet Medix Laboratoriot Oy

• Finland
• Hospitals and Health Care
• 1 - 100 Employee

• Substitute of a Bioanalyst

  • 2007 - 2009

  Substituted a bioanalyst during three summers in 2007-2009. - Performed and analyzed cytogenetic and molecular genetic diagnostic laboratory tests on patient samples in the Genetics department (ISO 17025 accreditation) - Cultured cells, fixed and G-band stained chromosomes from tissues (blood, placenta, amniotic fluid, bone marrow) - Analyzed chromosomes and karyotypes utilizing Ikaros-software - Automated blood DNA extraction - FISH-microdeletion analysis and Taqman PCR… Show more Substituted a bioanalyst during three summers in 2007-2009. - Performed and analyzed cytogenetic and molecular genetic diagnostic laboratory tests on patient samples in the Genetics department (ISO 17025 accreditation) - Cultured cells, fixed and G-band stained chromosomes from tissues (blood, placenta, amniotic fluid, bone marrow) - Analyzed chromosomes and karyotypes utilizing Ikaros-software - Automated blood DNA extraction - FISH-microdeletion analysis and Taqman PCR (MRSA and lactose intolerance tests) Show less Substituted a bioanalyst during three summers in 2007-2009. - Performed and analyzed cytogenetic and molecular genetic diagnostic laboratory tests on patient samples in the Genetics department (ISO 17025 accreditation) - Cultured cells, fixed and G-band stained chromosomes from tissues (blood, placenta, amniotic fluid, bone marrow) - Analyzed chromosomes and karyotypes utilizing Ikaros-software - Automated blood DNA extraction - FISH-microdeletion analysis and Taqman PCR… Show more Substituted a bioanalyst during three summers in 2007-2009. - Performed and analyzed cytogenetic and molecular genetic diagnostic laboratory tests on patient samples in the Genetics department (ISO 17025 accreditation) - Cultured cells, fixed and G-band stained chromosomes from tissues (blood, placenta, amniotic fluid, bone marrow) - Analyzed chromosomes and karyotypes utilizing Ikaros-software - Automated blood DNA extraction - FISH-microdeletion analysis and Taqman PCR (MRSA and lactose intolerance tests) Show less