Sylviane Yoba, Ph.D.

Scientist at LifeArc
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Contact Information
us****@****om
(386) 825-5501
Location
Stevenage, England, United Kingdom, UK
Languages
  • Français Native or bilingual proficiency
  • Anglais Full professional proficiency
  • Allemand Limited working proficiency
  • Espagnol Limited working proficiency
  • Japonais Limited working proficiency

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Credentials

  • iPhotography Course
    The CPD Certification Service
    Feb, 2014
    - Nov, 2024

Experience

  • LifeArc
    • United Kingdom
    • Non-profit Organizations
    • 200 - 300 Employee
    • Scientist
      • Jan 2021 - Present

      - Screened hundreds of small molecules and antibodies targeting pathways involved in cancer. - Developed functional assays (cell killing, cytokine release, platelet assay, internalization assay, binding assay) to select/characterize lead candidates. - Designed dozens of cell lines (primary, tumour cells) - Led the biology part of projects. Management of laboratory and equipment - Trained IP students in cell culture (T cells, CHO, HEK) - Organized monthly biology department meetings - Wrote project proposals. Provided strategic advice for projects. - Methodologies: cell culture, high content/high throughput automated screening, multi-colour (multiplex) FACS, ELISA (MSD), transfection, transduction, internalization assay, live imaging (Incucyte), luminescence, Octet, Viaflow, CDD Vault, Dotmatics, R/R Studio. Show less

  • University of Bristol
    • United Kingdom
    • Higher Education
    • 700 & Above Employee
    • Research associate in molecular microbiology British Heart Foundation
      • Aug 2017 - Oct 2020

      Established cellular pathways targeted by Streptococcus gordonii in platelets and endothelium in the context of infective endocarditis - Developped recombinant proteins from Streptococcus gordonii - Developped multiple assays to assess protein expression and platelet biology during S. gordonii/human platelet interactions - Established biological events in platelets infected with S. gordonii (GpVI, GpIb, P-selectin...) - Supervised students during research project or case base learning - Internal examiner during the APR of a PhD student - Methodologies: culture of bacteria human platelets and endothelial cells, phlebotomy, PCR, affinity chromatography, agarose/electrophoresis gel, confocal microscopy, in vivo imaging, ELISA, flow cytometry, aggregation assay, adhesion assay, mutagenesis, western blot, MS office, GraphPad Prism, ImageJ, FlowJo. Show less

  • Royal College of Surgeons in Ireland (RCSI)
    • Ireland
    • Higher Education
    • 700 & Above Employee
    • Visiting Researcher
      • Nov 2018 - Mar 2020

      - Established adhesins responsible for the activation of human aortic endothelium in presence of S. gordonii - Methodologies: bacterial culture, culture of human primary cells, infection, Ibidi system, in vivo imaging. - Established adhesins responsible for the activation of human aortic endothelium in presence of S. gordonii - Methodologies: bacterial culture, culture of human primary cells, infection, Ibidi system, in vivo imaging.

  • Queen's University Belfast
    • United Kingdom
    • Higher Education
    • 700 & Above Employee
    • Research assistant Marie Curie ITN
      • May 2013 - Dec 2016

      - Developed multiple in vitro assays to assess autophagy, evaluate gene/protein expression and survival in Klebsiella-infected macrophages in the context of respiratory infections and COPD - Designed and executed bioassays to evaluate the effect of drugs (mainly rapamycin and H89) on biological events such as autophagy, cell death, survival, gene/protein expression, cell signaling - Established biological events and signaling pathways affected in the host during Klebsiella-induced infections (signaling pathways, virulence factors, autophagy...) - Designed cell lines to assess protein expression during Klebsiella-induced infections - Extensive culture of immortalized and primary cells - Trained scientific and technical staff (immunocytochemistry, microscopy, western blot) - Organised/chaired lab meetings/conferences/science festivals/symposiums - Methodologies: microbial culture, bacterial selection, gene induction, plasmid purification, transfection, bioengineering, culture of immortalized and primary cells (alveolar macrophages, lung epithelial cells), infection, cell death, intracellular survival assay, nuclear extraction, DNA/RNA extraction, PCR, RT-qPCR, BCA assay, agarose/electrophoresis gel, immunocytochemistry, fluorescence microscopy, confocal microscopy, western blot, primer design, MS office, GraphPad Prism, ImageJ, BLAST. Show less

  • Institut Pasteur
    • France
    • Research Services
    • 700 & Above Employee
    • Visiting research assistant
      • Sep 2014 - Nov 2014

      Developed an in vitro assay to assess autophagy during K. pneumoniae-induced infections. Methodologies: cell culture, microscopy (confocal, Apotome), live imaging (spinning disk). Developed an in vitro assay to assess autophagy during K. pneumoniae-induced infections. Methodologies: cell culture, microscopy (confocal, Apotome), live imaging (spinning disk).

  • Science Teacher
    • Région de Paris, France
    • Science Tutor
      • Jan 2012 - Apr 2013

      Private tutor: biology, mathematics, chemistry, physics, English, French to secondary school students. Private tutor: biology, mathematics, chemistry, physics, English, French to secondary school students.

  • Animal Experimentation
    • University of Reims
    • Certificate of Animal Experimentation level II (training)
      • Sep 2012 - Sep 2012

      On rats, mice and rabbits. Handling, anesthesia, injections, blood samples, restraints On rats, mice and rabbits. Handling, anesthesia, injections, blood samples, restraints

  • Etablissement Français du Sang
    • Creteil, FRANCE
    • Cell culture of mesenchymal stromal cells (MSC) from human umbilical cord (training)
      • May 2012 - May 2012

      Elaboration, maintenance of cell lines, phenotypic analysis of human MSC to see which proteins they express. Dosage of VEGF. Methodologies: ELISA, cell culture, flow cytometry. Elaboration, maintenance of cell lines, phenotypic analysis of human MSC to see which proteins they express. Dosage of VEGF. Methodologies: ELISA, cell culture, flow cytometry.

  • Harvard Medical School
    • United States
    • Higher Education
    • 700 & Above Employee
    • Research assistant in immunology
      • Oct 2010 - May 2011

      Characterized double negative T cells in sooty mangabeys (controllers of SIV). Methodologies: isolation of PBMCs, flow cytometry, isolation of RNA, PCR, agarose gel, cloning, sequencing, bioinformatics. Characterized double negative T cells in sooty mangabeys (controllers of SIV). Methodologies: isolation of PBMCs, flow cytometry, isolation of RNA, PCR, agarose gel, cloning, sequencing, bioinformatics.

  • Paris-Diderot University, Institut de Biologie des Plantes
    • Gif-sur-Yvette. Laboratory of François Bouteau
    • Research assistant in biology
      • Jun 2010 - Jul 2010

      Evaluated the immunogenicity of LPS on tobacco and Arabidopsis thaliana cells following an infection with Pectobacterium caratovorum and Pectobacterium astrosepticum (production of ROS and calcium, cell death assays). Methodologies: cell culture (plant/bacteria), infection, luminometry, Trypan Blue. Evaluated the immunogenicity of LPS on tobacco and Arabidopsis thaliana cells following an infection with Pectobacterium caratovorum and Pectobacterium astrosepticum (production of ROS and calcium, cell death assays). Methodologies: cell culture (plant/bacteria), infection, luminometry, Trypan Blue.

Education

  • Queen's University Belfast
    Doctor of Philosophy (Ph.D.), Sciences microbiologiques et immunologie
    2013 - 2016
  • Université de Reims
    Expérimentation animale niveau 2
    2012 - 2012
  • Université Paris-Diderot/ Institut Pasteur
    Master, Immunologie, Biochimie, Recherche
    2009 - 2011
  • Université Paris-Diderot
    Licence de Biologie Cellulaire/Moléculaire et Physiologie, Biologie cellulaire/moléculaire, biologie du développement, neurosciences, biostatistiques
    2005 - 2009
  • Lycée Flora Tristan
    Baccalauréat scientifique option physique-chimie, Mention assez-bien
    2005 - 2005
  • Université Paris-Est Créteil (UPEC)
  • Université Paris-Est Créteil (UPEC)
  • Université Paris-Est Créteil (UPEC)
  • Université Paris-Est Créteil (UPEC)
  • Université Paris-Est Créteil (UPEC)

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