Experience

Guardant Health

• United States
• Biotechnology Research
• 700 & Above Employee

• Scientist I, IVD/CDx

  • Mar 2023 - Present

• Staff Research Associate

  • Feb 2020 - Feb 2023



Cepheid

• United States
• Biotechnology Research
• 700 & Above Employee

• Associate Scientist

  • Mar 2018 - Feb 2020

  • Develop multiplex 10-color MTB/XDR Xpert assay for Gene Xpert system. • Optimize real time PCR and RT-PCR assays on Omni system. • Perform analytical studies required for design verification of complex assays for CE-IVD and US-IVD. • Help develop strategies for development of complex in vitro diagnostic tests. • Develop and write verification and validation SOPs, technical reports and study plans following CLSI guideline. • Execute several research projects involved BCG cells… Show more • Develop multiplex 10-color MTB/XDR Xpert assay for Gene Xpert system. • Optimize real time PCR and RT-PCR assays on Omni system. • Perform analytical studies required for design verification of complex assays for CE-IVD and US-IVD. • Help develop strategies for development of complex in vitro diagnostic tests. • Develop and write verification and validation SOPs, technical reports and study plans following CLSI guideline. • Execute several research projects involved BCG cells and clinical specimens such as sputum. • Present data and results internally through departmental presentation and team meetings. Show less • Develop multiplex 10-color MTB/XDR Xpert assay for Gene Xpert system. • Optimize real time PCR and RT-PCR assays on Omni system. • Perform analytical studies required for design verification of complex assays for CE-IVD and US-IVD. • Help develop strategies for development of complex in vitro diagnostic tests. • Develop and write verification and validation SOPs, technical reports and study plans following CLSI guideline. • Execute several research projects involved BCG cells… Show more • Develop multiplex 10-color MTB/XDR Xpert assay for Gene Xpert system. • Optimize real time PCR and RT-PCR assays on Omni system. • Perform analytical studies required for design verification of complex assays for CE-IVD and US-IVD. • Help develop strategies for development of complex in vitro diagnostic tests. • Develop and write verification and validation SOPs, technical reports and study plans following CLSI guideline. • Execute several research projects involved BCG cells and clinical specimens such as sputum. • Present data and results internally through departmental presentation and team meetings. Show less



Agilent Technologies

• Biotechnology Research
• 700 & Above Employee

• Associate Researcher

  • Sep 2016 - Oct 2017

  - Performed targeted genome editing using CRISPR/Cas9 system and test the characteristics of different gRNA on different cell lines. - Conducted PCR, qPCR, RT-PCR and prepare cDNA, DNA libraries for single read or pair-end read sequencing using Illumina NGS. - Assessed and troubleshoot problems regarding DNA quality and quantity. - Performed high through put screening using variety of automatic system and instruments (Bioanalyzer, Tapestation, Qiacube). - Performed targeted genome editing using CRISPR/Cas9 system and test the characteristics of different gRNA on different cell lines. - Conducted PCR, qPCR, RT-PCR and prepare cDNA, DNA libraries for single read or pair-end read sequencing using Illumina NGS. - Assessed and troubleshoot problems regarding DNA quality and quantity. - Performed high through put screening using variety of automatic system and instruments (Bioanalyzer, Tapestation, Qiacube).



Varian

• United States
• Medical Device
• 700 & Above Employee

• Intern - Biology Scientist

  • May 2016 - Sep 2016

  • Worked with different cancer cell lines, prepared cell culture and maintained the cells for experiments. • Conducted gammaH2AX ELISA assay (Trevigen), cell toxicity, cell proliferation assay (Promega) and clonogenic assay to investigate the effect of DNA repair pathway’s inhibitors in glioblastoma cell lines and the level of DNA damage under different drug and radiation treatment. • Determined half inhibitory concentration for different cancer cell line with different drug treatment… Show more • Worked with different cancer cell lines, prepared cell culture and maintained the cells for experiments. • Conducted gammaH2AX ELISA assay (Trevigen), cell toxicity, cell proliferation assay (Promega) and clonogenic assay to investigate the effect of DNA repair pathway’s inhibitors in glioblastoma cell lines and the level of DNA damage under different drug and radiation treatment. • Determined half inhibitory concentration for different cancer cell line with different drug treatment (Origin). • Presented data and results internally through departmental presentation and team meetings. Show less • Worked with different cancer cell lines, prepared cell culture and maintained the cells for experiments. • Conducted gammaH2AX ELISA assay (Trevigen), cell toxicity, cell proliferation assay (Promega) and clonogenic assay to investigate the effect of DNA repair pathway’s inhibitors in glioblastoma cell lines and the level of DNA damage under different drug and radiation treatment. • Determined half inhibitory concentration for different cancer cell line with different drug treatment… Show more • Worked with different cancer cell lines, prepared cell culture and maintained the cells for experiments. • Conducted gammaH2AX ELISA assay (Trevigen), cell toxicity, cell proliferation assay (Promega) and clonogenic assay to investigate the effect of DNA repair pathway’s inhibitors in glioblastoma cell lines and the level of DNA damage under different drug and radiation treatment. • Determined half inhibitory concentration for different cancer cell line with different drug treatment (Origin). • Presented data and results internally through departmental presentation and team meetings. Show less



UCSF Smith Cardiovascular Institute

• San Francisco, CA

• Graduate Research Assistant

  • Sep 2015 - Feb 2016

  • Conducted analyses and prepared primary cell digestion, buffer, and chemical solutions. • Prepared live cell imaging using fluorescence microscopy and perform image analysis and cell counts (ImageJ). • Conducted routine animal husbandry in assisting other life science researchers. • Performed DNA extraction, mice genotyping, PCR and analysis of PCR using gel electrophoresis. • Conducted analyses and prepared primary cell digestion, buffer, and chemical solutions. • Prepared live cell imaging using fluorescence microscopy and perform image analysis and cell counts (ImageJ). • Conducted routine animal husbandry in assisting other life science researchers. • Performed DNA extraction, mice genotyping, PCR and analysis of PCR using gel electrophoresis.



Sanmina

• United States
• Appliances, Electrical, and Electronics Manufacturing
• 700 & Above Employee

• Chemical Analyst

  • May 2014 - Sep 2015

  * Performed quality control by conducting several routine chemical analyses of raw materials, wasted water , etching and plating solutions according to Standard Operation Procedures (SOPs). * Maintained laboratory inventory and prepared laboratory stock solutions, buffers and chemicals, changed chemical baths and consolidated chemical wastes. * Collaborated with Quality Assurance to redesigned lab reports, documentations and implemented more effective lab procedures. * Interpreted… Show more * Performed quality control by conducting several routine chemical analyses of raw materials, wasted water , etching and plating solutions according to Standard Operation Procedures (SOPs). * Maintained laboratory inventory and prepared laboratory stock solutions, buffers and chemicals, changed chemical baths and consolidated chemical wastes. * Collaborated with Quality Assurance to redesigned lab reports, documentations and implemented more effective lab procedures. * Interpreted data, performed laboratory investigations, remedied cause of deviation and reported scientific results to supervisors. Show less * Performed quality control by conducting several routine chemical analyses of raw materials, wasted water , etching and plating solutions according to Standard Operation Procedures (SOPs). * Maintained laboratory inventory and prepared laboratory stock solutions, buffers and chemicals, changed chemical baths and consolidated chemical wastes. * Collaborated with Quality Assurance to redesigned lab reports, documentations and implemented more effective lab procedures. * Interpreted… Show more * Performed quality control by conducting several routine chemical analyses of raw materials, wasted water , etching and plating solutions according to Standard Operation Procedures (SOPs). * Maintained laboratory inventory and prepared laboratory stock solutions, buffers and chemicals, changed chemical baths and consolidated chemical wastes. * Collaborated with Quality Assurance to redesigned lab reports, documentations and implemented more effective lab procedures. * Interpreted data, performed laboratory investigations, remedied cause of deviation and reported scientific results to supervisors. Show less