Experience

University of Colorado Anschutz Medical Campus

• United States
• Higher Education
• 700 & Above Employee

• Instructor

  • Aug 2021 - Present



University of Colorado

• United States
• Higher Education
• 700 & Above Employee

• Research Associate

  • Jul 2011 - Present

  During present postdoctoral study, the anti-CRC efficacy of silibinin has been evaluated extensively with central emphasis on targeting both colon CSC/bulk tumor cells. Herein, we assessed the potential of silibinin to eradicate colon CSC and its associated molecular mechanisms. Silibinin significantly decreased colon CSC pool together with cell survival of bulk tumor cells. These silibinin effects were associated with decreased mRNA and protein levels of various CSC-associated transcription factors, signaling molecules and markers. Furthermore, 2D and 3D differentiation assays indicated formation of more differentiated clones by silibinin. Our findings also showed silibinin potential to interfere with self-renewal of CSC pool by shifting CSC cell division from symmetric to asymmetric or symmetric commitment type via targeting various signals associated with the survival and multiplication of colon CSC pool.However, the above observations were under in vitro conditions. To overcome the limitations of these findings, we assessed oral silibinin effect on tumorigenic potential of colon CSC. To determine oral silibinin effect on CSC self-renewal in serial transplantation studies, these CSC enriched fractions and the unsorted population of tumor cells were transplanted back into NOD/SCID mice and investigated for initiation, growth, and tumor phenotype, with or without silibinin treatment. Sorted CSC and unsorted tumor cells from both control and silibinin-treated mice were also subjected to in vitro experiments to determine their self- renewal and differentiation capacity. We observed that silibinin feeding has an inhibitory effect on tumorigenic potential of colon CSC along with a decrease in latency as well as size/ volume of tumor. We also studied epigenetics by evaluation of DNA methylation, miRNA, histone acetylation/methylation.



University of North Texas

• United States
• Higher Education
• 700 & Above Employee

• Research Associate

  • Aug 2010 - Jun 2011

  Resveratrol has been reported as an antioxidant agent. Whether the effects of oxidative stress induced by 4-HNE could be attenuated in cultured human lens epithelial cells by incubation with resveratrol is still unknown. Our primarily investigation suggested that resveratrol protected HLEB-3 Wt cells from 4-HNE induced oxidative damage; however synergistic effect was observed in GST 4-4A transfected cell. Resveratrol has been reported as an antioxidant agent. Whether the effects of oxidative stress induced by 4-HNE could be attenuated in cultured human lens epithelial cells by incubation with resveratrol is still unknown. Our primarily investigation suggested that resveratrol protected HLEB-3 Wt cells from 4-HNE induced oxidative damage; however synergistic effect was observed in GST 4-4A transfected cell.



School of Biotechnology, BHU

• Ph.D.

  • 2004 - 2010

  Helicobacter pylori is a highly successful bacterial pathogen that persistently colonizes the mucosa of the human stomach. I have made an attempt to reveal certain facts related to the factors involved in pathogenecity and the role of host factors in appearance and development of gastric diseases caused by H. pylori. For direct detection of H. pylory and its genetic diversity study we developed a novel multiplex PCR and for its quantitative estimation we also developed a competitive PCR. The study involved 276 cases, molecular diversity analysis was conducted with H. pylori isolates by Restriction Fragment Length Polymorphism (RFLP) of 16S rRNA gene, Enterobacterial Repetitive Intergenic Consensus (ERIC)–PCR and Amplified Fragment Length Polymorphism (AFLP). All the isolates showed distinguishable banding pattern by AFLP, genetic diversity based on 16S rDNA sequences suggested that Indian isolates are closely related to the isolates of Brazil and/or Taiwan. The relationship between polymorphism in interleukin 1B (IL-1B), H. pylori infection, and gastric cancer (GC) was also evaluated. The frequency of IL-1RN 2/2 was significantly higher in GC cases than the controls. The risk of gastric cancer markedly increased in the genotypes of IL-1B -511 TT, -31CC, +3954 CT and IL-1RN 1/2 when infected with H. pylori.