Bioarchitect and Interim Lab Manager

• Feb 2023 - Present

Associate Scientist

• Jan 2022 - Aug 2023

Associate Scientist

• Mar 2021 - Jan 2022

Gene therapy for acquired an metabolic liver diseases. Internalized development of BioXP for in-house gene synthesis. Designed, cloned, tested, and screened plasmids of different types of transgenes, including vectorized antibodies with different architectures and regulatory elements; analyzed secretion of vectorized antibodies by transfection in mammalian cells and ELISA; developed antibody-antigen avidity ELISAs. Interdisciplinary roles with AAV core, Bioanalytical, and Pharmacology, with… Show more Gene therapy for acquired an metabolic liver diseases. Internalized development of BioXP for in-house gene synthesis. Designed, cloned, tested, and screened plasmids of different types of transgenes, including vectorized antibodies with different architectures and regulatory elements; analyzed secretion of vectorized antibodies by transfection in mammalian cells and ELISA; developed antibody-antigen avidity ELISAs. Interdisciplinary roles with AAV core, Bioanalytical, and Pharmacology, with in vitro testing to initiate in vivo studies; delivered test articles at scale for in vivo studies. Provided regular updates to leadership, including executive team and co-founders. Show less Gene therapy for acquired an metabolic liver diseases. Internalized development of BioXP for in-house gene synthesis. Designed, cloned, tested, and screened plasmids of different types of transgenes, including vectorized antibodies with different architectures and regulatory elements; analyzed secretion of vectorized antibodies by transfection in mammalian cells and ELISA; developed antibody-antigen avidity ELISAs. Interdisciplinary roles with AAV core, Bioanalytical, and Pharmacology, with… Show more Gene therapy for acquired an metabolic liver diseases. Internalized development of BioXP for in-house gene synthesis. Designed, cloned, tested, and screened plasmids of different types of transgenes, including vectorized antibodies with different architectures and regulatory elements; analyzed secretion of vectorized antibodies by transfection in mammalian cells and ELISA; developed antibody-antigen avidity ELISAs. Interdisciplinary roles with AAV core, Bioanalytical, and Pharmacology, with in vitro testing to initiate in vivo studies; delivered test articles at scale for in vivo studies. Provided regular updates to leadership, including executive team and co-founders. Show less

Research Associate IV

• Jan 2019 - Mar 2021

Contributed to IND filings for Factor IX, MPS I, MPS II, and Fabry. Screened zinc-finger nuclease constructs in vitro with indel analysis by next-gen sequencing. Assessed donor transgene expression in vitro and in vivo by deep sequencing, RT-qPCR, Western blot, ELISA, and enzyme activity assays. Performed flow cytometry for target integration analysis using GFP-expressing vectors. Cloned plasmids for ZFNs and donor constructs for AAV production. Examined AAV quality by capsid ELISA, titer… Show more Contributed to IND filings for Factor IX, MPS I, MPS II, and Fabry. Screened zinc-finger nuclease constructs in vitro with indel analysis by next-gen sequencing. Assessed donor transgene expression in vitro and in vivo by deep sequencing, RT-qPCR, Western blot, ELISA, and enzyme activity assays. Performed flow cytometry for target integration analysis using GFP-expressing vectors. Cloned plasmids for ZFNs and donor constructs for AAV production. Examined AAV quality by capsid ELISA, titer by ddPCR and qPCR, and alkaline gels. Interfaced with facilities, vendors, and end-users during set-up and ongoing maintenance of new lab space in Brisbane.

Research Associate III

• Aug 2012 - Jan 2019

Research Associate III

• Jun 2012 - Aug 2012

Staff Research Associate II

• Apr 2008 - May 2012

Examined prion strain propagation in mammalian cell culture; distinguished the strains by drug sensitivity, guanidine stability, phosphotungstic acid precipitation, and Proteinase K digest pattern. Developed and implemented protocols for in-house production of 15-N Spirulina platensis for metabolic labeling in protein turnover studies, a cost savings of 90% compared to commercially available 15-N Spirulina platensis. Examined PrP trafficking to lipid rafts in mammalian cells under drug… Show more Examined prion strain propagation in mammalian cell culture; distinguished the strains by drug sensitivity, guanidine stability, phosphotungstic acid precipitation, and Proteinase K digest pattern. Developed and implemented protocols for in-house production of 15-N Spirulina platensis for metabolic labeling in protein turnover studies, a cost savings of 90% compared to commercially available 15-N Spirulina platensis. Examined PrP trafficking to lipid rafts in mammalian cells under drug treatments. Trained postdocs in mammalian cell culture, Western blot, and ELISA. Purified recombinant Fabs by FPLC; purified recombinant PrP by HPLC. Processed and catalogued human plasma in BSL-3 for clinical collaboration on rapidly progressive dementia. Consolidated and placed weekly supply orders. Built a searchable database of cell stocks in liquid nitrogen storage. Show less Examined prion strain propagation in mammalian cell culture; distinguished the strains by drug sensitivity, guanidine stability, phosphotungstic acid precipitation, and Proteinase K digest pattern. Developed and implemented protocols for in-house production of 15-N Spirulina platensis for metabolic labeling in protein turnover studies, a cost savings of 90% compared to commercially available 15-N Spirulina platensis. Examined PrP trafficking to lipid rafts in mammalian cells under drug… Show more Examined prion strain propagation in mammalian cell culture; distinguished the strains by drug sensitivity, guanidine stability, phosphotungstic acid precipitation, and Proteinase K digest pattern. Developed and implemented protocols for in-house production of 15-N Spirulina platensis for metabolic labeling in protein turnover studies, a cost savings of 90% compared to commercially available 15-N Spirulina platensis. Examined PrP trafficking to lipid rafts in mammalian cells under drug treatments. Trained postdocs in mammalian cell culture, Western blot, and ELISA. Purified recombinant Fabs by FPLC; purified recombinant PrP by HPLC. Processed and catalogued human plasma in BSL-3 for clinical collaboration on rapidly progressive dementia. Consolidated and placed weekly supply orders. Built a searchable database of cell stocks in liquid nitrogen storage. Show less

Staff Research Assistant

• May 2007 - Apr 2008

Habituated rats to alcohol consumption through intermittent access to 20% ethanol in the home cage; subsequently trained the rats in operant self-administration chambers for alcohol consuption; recorded data on alcohol consumption during the training regimens. Regularly handled and injected rats and mice to minimized stress effects in behavioral studies. Served as intermediary between the research team and the mouse colony manager to allocate animals for experiments. Cryosectioned perfused… Show more Habituated rats to alcohol consumption through intermittent access to 20% ethanol in the home cage; subsequently trained the rats in operant self-administration chambers for alcohol consuption; recorded data on alcohol consumption during the training regimens. Regularly handled and injected rats and mice to minimized stress effects in behavioral studies. Served as intermediary between the research team and the mouse colony manager to allocate animals for experiments. Cryosectioned perfused rodent prains for histology. Show less Habituated rats to alcohol consumption through intermittent access to 20% ethanol in the home cage; subsequently trained the rats in operant self-administration chambers for alcohol consuption; recorded data on alcohol consumption during the training regimens. Regularly handled and injected rats and mice to minimized stress effects in behavioral studies. Served as intermediary between the research team and the mouse colony manager to allocate animals for experiments. Cryosectioned perfused… Show more Habituated rats to alcohol consumption through intermittent access to 20% ethanol in the home cage; subsequently trained the rats in operant self-administration chambers for alcohol consuption; recorded data on alcohol consumption during the training regimens. Regularly handled and injected rats and mice to minimized stress effects in behavioral studies. Served as intermediary between the research team and the mouse colony manager to allocate animals for experiments. Cryosectioned perfused rodent prains for histology. Show less