Experience

Clinical Research Management, Inc. (ClinicalRM)

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Assistant IV (a contractor at walter Reed Army Institute of Research)

  • Nov 2014 - Present

  •Study blasts induce brain injury and hearing loss. •Perform duties required to detect, identify, and quantify neurobiological mediators involved in the pathophysiology of bTBI using appropriate molecular analytical techniques. •Independently develop experimental methods, perform data analysis, and report results. •Recommend equipment acquisitions appropriate for experimental procedures and maintain a laboratory inventory of chemical reagents and surgical supplies required for these tasks, as well as ensuring an organized and functional laboratory is maintained. The main techniques are used in the lab: •Isolation and purification (extraction) of RNA (from tissue and cells); quantification of RNA by using RT-PCR and qPCR. •Purification of protein (from tissue and cells), western blotting, immunohistochemistry and ELISA. •Tissue culture (isolated primary cells from the brain of the mouse embryos). •Transfection by using lipofection with Crispr/cas9 vectors and other plasmid vectors, and transduction by using viral particle. Show less



Children's National Hospital

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Assitant III in Research Center for Genetic Medicine

  • Jul 2011 - Oct 2014

  •Perform cell culture, nucleic acid extraction, RT-qPCR, western blotting, flow cytometry, 2D gel and SILAC assay etc. to define effects of hypoxia on modification of ADAMTS13 and cell cycle progression and proliferation of airway epithelial. •Maintain and generate cell line and cell bank (include patient's samples). •Train new employees and oversee lab supplies

• Research Assistant III in Resaerch Center for Cancer and Immunology

  • Dec 2005 - Jun 2011

  •Developed tumor cell models of absent ganglioside by using transfection technique with retrovirus vector, viral particle shRNA; generated cell lines from mouse embryo, skin, tumor tissue, and human skin of absent ganglioside; conducted RT-qPCR, cell proliferation assay, ELISA and lipid assay; isolation single cell from tumor, spleen and lymph node of the transgenic/ knockout mouse to test function of immune cells (MDSC, T cell and B cell…) by flow cytometry etc. to study tumor host interaction in tumor microenvironment. •Extracted DNA, RNA, and protein from cells and various tissues of mouse •Maintained cell lines and cell bank •Maintained normal and knockout/transgenic mice strains. •Trained new employees and order lab supplies. Show less

• Research Assistant II&III in Research Center for Cancer and Immunology

  • May 2001 - Nov 2005

  •Performed transient transfection, RNA interference (siRNA), RT-qPCR, in vitro transcription and cellular fractionation of protein to study regulation of Human cytomegalovirus (HCMV) IE gene expression, trafficking and function of HCMV UL37 protein. •Performed site-directed mutagenesis, DNA sequencing etc. to construct recombinant DNA molecules and clone DNA fragments. •Generated and titrated viral stocks. •Trained new employees and Order lab supplies.