Steve Stiehl

Research Associate at R&D Systems
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Contact Information
us****@****om
(386) 825-5501
Location
St Paul, Minnesota, United States, US
Skills

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Credentials

  • Supervision 1 training
    -

Experience

  • R&D Systems
    • United States
    • Biotechnology Research
    • 300 - 400 Employee
    • Research Associate
      • Jun 2013 - Present

      Experimental Design/Biological Validation Research Associate Run Luminex and MAGPIX multiplexing units for analysis. Trouble shoot multiplexing machines with my own experimental designs. Run Western Blots and analyze data to assist other departments with enhancing their experimental results. Provide data for the product datasheets. Proficient in immunoprecipitation and chemoluminescence techniques. Conduct research of scientific papers and journals to create more streamlined experiments and research Prepare cell cultures for testing

    • Research Associate 4
      • May 2006 - Jun 2013

      Product Support Group Research Associate Level 3 and 4 Designed and created experiments to resolve issues with buffers, plate coating, reagent preps, purification techniques and development issues related to immunoassays. Resolved problems with the ELISA and Duoset kits. Personally designed many unique resolutions and ideas that improved and fixed the current issues. Developed and implemented over 60 new documents and procedures for SOP’s . In charge of many redevelopment and research projects Organized and facilitated meetings related to redevelopment and troubleshooting projects.  Worked collaboratively with all departments downstream and upstream of production to effectively and efficiently resolve the most difficult problems. Solved customer issues and complaints in a timely manner.  Knowledgeable with both antigen and antibody down ELISA’s. Designed a new process to separate the monomer and the dimer of a protein that had not been previously used within the company.

    • Research Associate
      • Jun 1994 - May 2006

      Antibody Purification Research Associate Level 2 and 3 Purify Monoclonal and Polyclonal antibodies by antigen affinity, protein A, and protein G. Performed horseradish peroxidase (HRP) and streptavidin alkaline phosphatase (AP) conjugation. Oversaw 30 AKTA FPLC, Purifiers and HPLC machines.  Performed maintenance, repairs and designed all programs for purifications, including size exclusion, gradients, ion exchange, and antigen affinity columns. Designed programs on the AKTA (Unicorn/Wizard) and AKTA Prime Plus (PrimeView) Familiar with multiple purification stations including AKTA, Waters, and Perkin Elmer.  Performed cleavages of –Fc fusion proteins for immunization by factor Xa.  Proficient in a wide variety of purification methods:• Affinity, ion exchange, and size exclusion. Prepared small and large scale antibody purification (ug – g) while maintaining purity and quality. Concentrated proteins using ultrafiltration (Amicon stirred cells).

    • Manufacturing Associate / Team Lead
      • Dec 1991 - Jun 1994

      Manufacturing Associate Level 1 Team Lead Primary person in charge of making all buffers for plate coating and reagents. In charge of the weekly planning meeting for the manufacturing of ELISA kits and their components. Helped to set up the original production facility for the diagnostic division as well as instrumental in the transfer and assembly of the AMGEN acquisition.

  • Amgen
    • United States
    • Biotechnology Research
    • 700 & Above Employee
    • Manufacturing Associate 1
      • May 1989 - Nov 1991

      Manufacturing Associate Level 1 Assisted and executed the setup of the entire diagnostic manufacturing facility.  Formulated all materials for the diagnostic division, created all the manufacturing documents, in charge of shipping, and receiving.  Corresponded with the research and development division to make a viable product. Manufacturing Associate Level 1 Assisted and executed the setup of the entire diagnostic manufacturing facility.  Formulated all materials for the diagnostic division, created all the manufacturing documents, in charge of shipping, and receiving.  Corresponded with the research and development division to make a viable product.

Education

  • Colorado State University
    Bachelor of Science - BS, Biochemistry
    1985 - 1990
  • Minneapolis Community and Technical College
    Associate's degree, Criminal Justice/Police Science
    1999 - 2000
  • Colorado State University
    BS, biochemistry
    1985 - 1990
  • Colorado State University
    Bachelor of Science - BS, Biochemistry
    -

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