Experience

R&D Systems

• United States
• Biotechnology Research
• 300 - 400 Employee

• Scientist

  • Jan 2007 - Present

  Challenging protein expression in CHO-S, HEK293, NSO, Per C6 cell lines. Examples include BMP's, Wnt's and Semaphorin protein expression, using above cell lines. Contribute to the development of new products by incorporating technologies related to recombinant protein expression in eukaryotic cells. My other responsibilities focus on analyzing the structure and characteristics of complex proteins in order to design efficient recombinant expression strategies. This may include gene modification as well as possible design and construction of expression vectors used for a broad range of protein expression in multiple systems. I also interact with other departments in order to facilitate effective progress of candidate reagents through various stages of product development.



Abbott

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Molecular biologist

  • Jan 2006 - Jun 2006

  Extensive experience in Cloning, expression of proteins in E.coli, Pichia vector construction and expression, insect cells and mammalian cells and subsequent protein isolation of N-His and C-His proteins and characterization, Performing all standard molecular biology laboratory procedures including Gateway cloning, 96 well cloning procedures, synthetic gene construction and site directed mutagenesis. Day to day use of VectorNTI,, as well as expertise in protein isolation and characterization using standard biochemical laboratory techniques. Additional responsibilities include providing regular updates, documentation, and communication of project results as appropriate, participating in national scientific meetings, and keeping current with field of expertise, including literature and technology development in the area of molecular biology.



Northwestern Medical school/ CMRC

• Senior Post doc

  • Dec 1997 - Jun 2006

  Virology Techniques (Baculo virus, Adeno virus and Adeno associated virus):Adeno viral construction using homologous recombination and ligation methods, Viral purification using CSCl centrifugation, Plaque assays and recombinant vector detection. Construction of several adeno associated viral vectors, viral purification (Iodoxinol step gradient centrifugation and FPLC method) and titering using ELISA and QRT-PCR. In vitro reporter assays and regulation (flow cytometry and QRT-PCR for detecting mRNA copy number) using regulatable promoters. Construction of baculoviral vectors for protein expression and AAV production. Growth of different types of bacteriophages, purification of DNA from different types of bacteriophages, cloning prokaryotic DNA into bacteriphage arms and packaging to form library, titering and plating of packaged library and library amplification. Growth and maintenance of ATCC 293, U20S, Hela, MCF10A, CHO, COS, 3T3 and SF9 cell lines. Over expression of proteins using adeno viral and baculo viral vectors. Construction of recombinant baculovirus vectors for making AAV vectors. Over expression of proteins in mammalian cells using CMV, SV 40 promoters, Infection of neuronal cell lines using replication defective lent viral vectors.



Gulbarga University

• Higher Education
• 1 - 100 Employee

• Student

  • 1984 - 1986

• Student

  • 1984 - 1986

• Student

  • 1984 - 1986

• Student

  • 1984 - 1986