Experience

Clinical Research Management, Inc. (ClinicalRM)

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Research Technician

  • Aug 2012 - Present

  • Working as lead technician on all studies related to RNA projects • Isolated RNA from Penetrating Ballistic-like Brain Injury (PBBI) samples • Conducted genomic preparations and analysis • Performed reverse transcriptase reactions and individual qPCR on several microRNA(miRNA) and messengerRNA (mRNA) targets on PBBI samples to confirm results from an openarray study • Organized miRNA sample data, executed experimental setup, planning, data management and graph analysis in software prism for this continuation segment of the study • Assisted in various traumatic brain injury (TBI) drug studies including administering drugs to laboratory rats, collection of biofluids, brain dissection, and sample processing • Expertise in sample prep and running western blots and MSD ELISA assays using brain tissue and bio-fluids from PBBI rats • Organized and analyzed raw experimental data using prism software and produced graphs using multiple t-tests and two-way ANOVA for correlating studies • Give weekly updates to PIs on progress of various studies being performed • Assisted in training new technicians on western blot and ELISA assays used for specific projects in addition to other various protocols relevant to research tasks Small Animal Expertise • Animal handling on all laboratory rats intended for study use • Oral gavage drug dosage on laboratory rats • Rat brain tissue collection and dissection • Ketamine injections/anesthesia on laboratory rats • Collect rat serum and plasma via cardiac puncture Administrative Skills • Drafted protocol and SOPs for various laboratory equipment relevant to research being conducted • Conducted procurement, logistics, database management of biological samples derived from human subjects • Completed all required ordering for laboratory reagents, stock supplies and equipment • Perform all necessary inventory for lab supplies and reagents



Claflin University

• United States
• Higher Education
• 300 - 400 Employee

• Graduate Researcher

  • Aug 2010 - May 2012

  • Use of microbiological and molecular techniques to construct mutants to identify genes in M. catarrhalis essential in protecting it from cationic antimicrobial peptides • Molecular techniques include plasmid construction, DNA manipulation, separation methods necessary in DNA analysis RNA isolation, PCR, cloning, primer design • Use of sequence scanner software to conduct gene sequencing and analysis to relevant research • Participated in regular meetings with supervisor to discuss, interpret and evaluate results from experiments relevant to research • Operation of laboratory equipment, such as centrifuge, pH meter, incubators, analytical balance and thermal cycler • Analyzed data from laboratory findings and prepared summaries for weekly laboratory meetings • Prepare various culture media, solutions and buffers • Experience using aseptic technique and microscopy • Provided training to undergraduate students on use of molecular laboratory equipment needed for experimental purposes



Financial and Realty Services, LLC

• Food and Drug Administration

• Laboratory Technician

  • Mar 2009 - Aug 2010

  • Prepared various culture media and buffer solutions for FDA laboratories • Conducted routine washing and sterilization • Performed quality control of microbiological culture media • Prepared standard operation procedures (SOPs) including protocol followed, correction of notes and documentation • Responsible for laboratory upkeep including regular calibration, cleaning, and preventive maintenance of laboratory equipment • Conducted proper disposal and handling of biological hazard waste products