Experience

A*STAR - Agency for Science, Technology and Research

• Singapore
• Research Services
• 700 & Above Employee

• Undergraduate Student Researcher

  • May 2023 - Present

  Engaging in my Final Year Project (FYP) under the Laboratory of Metabolic Medicine (LMM), IMCB, where I seek to investigate the role of branched-chain amino acid (BCAAs) and branched-chain aminotransferase (BCAT) in the development of hepatocellular carcinoma (HCC) - Trained to perform mammalian cell culture, protein purification, flow cytometry, as well as molecular techniques (e.g., DNA/RNA extraction, RT-qPCR, Molecular Cloning, Western blotting) Engaging in my Final Year Project (FYP) under the Laboratory of Metabolic Medicine (LMM), IMCB, where I seek to investigate the role of branched-chain amino acid (BCAAs) and branched-chain aminotransferase (BCAT) in the development of hepatocellular carcinoma (HCC) - Trained to perform mammalian cell culture, protein purification, flow cytometry, as well as molecular techniques (e.g., DNA/RNA extraction, RT-qPCR, Molecular Cloning, Western blotting)



Technion - Israel Institute of Technology

• Israel
• Higher Education
• 700 & Above Employee

• International Summer Research Programme

  • Jun 2023 - Jul 2023



NUS Department of Biological Sciences

• Singapore
• Higher Education
• 1 - 100 Employee

• Undergraduate Student Researcher

  • Sep 2022 - May 2023

  · Offered an opportunity to work as Undergraduate Student Researcher in Asst Prof Eunyoung Chae’s lab under NUS Student Work Scheme · Perform expression and purification of target proteins using E. coli expression system for use in characterization assays to identify cellular substrate, as well as the conditions required to modify biophysical properties of our protease, which was previously identified to be a NLR activator in A. thaliana. · Carry out high-throughput effector screening from multiple pathogens to identify numerous candidate effectors that can trigger hypersensitive response with our target protease in Nicotiana benthamiana (N. benthamiana) transient protein expression system Show less



A*STAR - Agency for Science, Technology and Research

• Singapore
• Research Services
• 700 & Above Employee

• Research Intern

  • May 2022 - Jul 2022

  A*GA - A*STAR Research Internship awardee attached to Institute of Molecular and Cell Biology (IMCB) - Acquired knowledge on the development process of monoclonal antibody (hybridoma generation, subclone selection, antibody purification from cell culture supernatant) - Perform molecular cloning to generate plasmids for expression of protein-of-interest in mammalian cell expression systems - Conducted routine characterization tests of generated mouse monoclonal antibodies against specific cancer and cytokine cell markers via sELISA, SDS-PAGE + Western blot, and immunofluorescence assay (IFA) - Experimented with the modification of antibodies to optimize their use for downstream diagnostic applications (e.g., immunohistochemistry (IHC) staining of tissue samples, IFA) Show less



NUS Department of Biological Sciences

• Singapore
• Higher Education
• 1 - 100 Employee

• Undergraduate Student Researcher

  • May 2021 - Apr 2022

  Undertaken 2 Undergraduate Research Opportunities in Science (UROPS) and received final report grades of A+ - Worked on the characterization of innate immunity activation pathways using Arabidopsis thaliana (A. thaliana) as model organism - Screened library of capsular polysaccharide (CPS) to identify potential polysaccharide molecular patterns that can initiate pattern-triggered immunity - Perform structure-guided mutagenesis at various sites of interest to give rise to different variants of known NLR interactors, and were transiently expressed together with its NLR pair to elucidate the biochemical pathway behind NLR activation leading to the observed autoimmunity phenomenon Show less