Sharon Manley
Research Scientist at Viracor Eurofins BioPharma Services- Claim this Profile
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Bio
Experience
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Eurofins Viracor BioPharma Services
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United States
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Biotechnology Research
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1 - 100 Employee
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Research Scientist
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Nov 2020 - Present
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University of Kansas Medical Center
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Higher Education
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700 & Above Employee
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Senior Research Associate
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Jan 2020 - Nov 2020
-Drug characterization studies in ALS bioenergetics. -Generation of induced pluripotent stem cells derived from Alzheimer patients.
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Postdoctoral Fellow
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Dec 2014 - Jan 2020
Awarded a KINBRE postdoctoral fellowship Successfully established utilization of Next Generation Sequencing for the laboratory for 16S rRNA sequencing and mitochondrial DNA sequencing. Demonstrated that mitochondria DNA cross talks with nuclear DNA to shape the gut microbiota by utilizing 16S rRNA sequencing (Next Generation Sequencing) and discovered that KISS1, a metastasis suppressor, activates fatty acid oxidation by inhibiting AMPK, an energy sensor
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University of Kansas Medical Center
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Higher Education
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700 & Above Employee
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Ph.D. Candidate
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Aug 2009 - Oct 2014
· Demonstrated that hydrophobic bile acids suppress hepatic autophagy · First to identify that bile acids sensor, FXR, is required for activation of FoxO3, a transcription factor, and subsequent hepatic autophagy activation in response to alcohol consumption · Established novel protocols, including measuring lysosomal activity using DQ-BSA in cellular lysate · · Demonstrated that hydrophobic bile acids suppress hepatic autophagy · First to identify that bile acids sensor, FXR, is required for activation of FoxO3, a transcription factor, and subsequent hepatic autophagy activation in response to alcohol consumption · Established novel protocols, including measuring lysosomal activity using DQ-BSA in cellular lysate ·
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Medical College of Wisconsin
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Higher Education
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700 & Above Employee
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Research Technologist
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Jul 2007 - Aug 2009
Expanded the human intestinal microvessel endothelial cell and human intestinal tissue banks in compliance with IRB and HIPAA and performed inflammatory bowel disease research using in vitro methods. Expanded the human intestinal microvessel endothelial cell and human intestinal tissue banks in compliance with IRB and HIPAA and performed inflammatory bowel disease research using in vitro methods.
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Marquette University
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United States
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Higher Education
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700 & Above Employee
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Research Trainee
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May 2006 - Aug 2006
· Defined the interaction domains of tRNA m1A methyltransferase using two-hybrid saccharomyces cerevisiae · Defined the interaction domains of tRNA m1A methyltransferase using two-hybrid saccharomyces cerevisiae
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Education
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University of Kansas School of Medicine
Doctor of Philosophy (Ph.D.), Toxicology -
Concordia University Wisconsin
Bachelor of Science (BS), Biology, General