Shannon Toffton

Senior Scientist-Microbiology at VitaKey Inc.
  • Claim this Profile
Contact Information
us****@****om
(386) 825-5501
Location
Raleigh, North Carolina, United States, US

Topline Score

Topline score feature will be out soon.

Bio

Generated by
Topline AI

You need to have a working account to view this content.
You need to have a working account to view this content.

Experience

    • United States
    • Biotechnology
    • 1 - 100 Employee
    • Senior Scientist-Microbiology
      • Nov 2021 - Present

      Durham, North Carolina, United States Leading efforts to evaluate novel microbial products. • Led efforts to purchase equipment and materials to establish microbiology laboratory. • Developing methods to assess viability of formulated probiotics in food products. • Overseeing shelf-life studies to evaluate formulated probiotic products in a variety of dairy matrices. • Serving as team lead with two direct reports.

    • United States
    • Biotechnology Research
    • 200 - 300 Employee
    • Scientist II
      • Jul 2019 - Nov 2021

      Raleigh-Durham, North Carolina Area Manufacturing DNA for use in gene therapy applications. Developing a scalable process and methods for production of DNA.

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Associate Research Scientist
      • Mar 2017 - Jul 2019

      Raleigh-Durham, North Carolina Area Developing and characterizing enzyme and microbial products for use in poultry and swine. Optimizing fermentation media and conditions to produce enzymes using bench scale bioreactors and shake flasks. Writing and validating SAPs/SOPs. Determining shelf life of microbial and enzyme products. Designing experiments to determine mode of action for probiotic products.

    • Denmark
    • Biotechnology Research
    • 700 & Above Employee
    • Research Associate II BioAG Microbial Discovery
      • Aug 2014 - Jan 2017

      RTP, NC Isolated and characterized beneficial microbes from environmental samples in support of BioAg pipeline. Extracted high-quality DNA from fungal and bacterial cultures. Utilized automated equipment for high throughput assays (liquid handlers and plate readers). Served as super user for MALDI-TOF Mass Spectroscopy System. • Decreased laboratory costs by integrating 2D Barcode tube sample tracking system into workflows. • Developed methods for isolating novel microbes from environmental… Show more Isolated and characterized beneficial microbes from environmental samples in support of BioAg pipeline. Extracted high-quality DNA from fungal and bacterial cultures. Utilized automated equipment for high throughput assays (liquid handlers and plate readers). Served as super user for MALDI-TOF Mass Spectroscopy System. • Decreased laboratory costs by integrating 2D Barcode tube sample tracking system into workflows. • Developed methods for isolating novel microbes from environmental samples. • Optimized workflow for bacterial and fungal isolations and assays. Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Technician/Lab Manager
      • Nov 2008 - Aug 2014

      Raleigh, NC Engaged in molecular toxicology research on DNA polymerases. Managed lab operations and safety. Performed mammalian and insect cell culture. Expressed proteins in yeast, bacteria, and cell lines. Developed novel qPCR assays. • Contributed to publication of 5 journal articles. • Arranged for repair and maintenance of laboratory equipment. • Created polymerase Eta mutants using site direction mutagenesis and cloning techniques. • Ensured safe lab operations by training new personnel… Show more Engaged in molecular toxicology research on DNA polymerases. Managed lab operations and safety. Performed mammalian and insect cell culture. Expressed proteins in yeast, bacteria, and cell lines. Developed novel qPCR assays. • Contributed to publication of 5 journal articles. • Arranged for repair and maintenance of laboratory equipment. • Created polymerase Eta mutants using site direction mutagenesis and cloning techniques. • Ensured safe lab operations by training new personnel on laboratory and safety protocols. Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Technician
      • Jul 2006 - Oct 2008

      Chapel Hill, NC Managed animal and laboratory resources. Purified and analyzed proteins from animal tissues. Ordered lab supplies and equipment. • Ensured laboratory compliance with animal rules and regulations. • Integrated new LIMS system to track availability of animals and progress of breeding programs. • Optimized PCR protocols for genotyping mice.

    • United States
    • Government Administration
    • 700 & Above Employee
    • Student Contractor
      • May 2005 - Jul 2006

      Durham, NC Assisted and engaged in developmental immunology and toxicology research. Conducted research involving rodent models. Prepared solutions and samples for experiments. Processed blood samples for ELISAs. • Assisted senior research personnel with complex animal assays.

Education

  • North Carolina State University
    BS, Microbiology
    2002 - 2006

Community

You need to have a working account to view this content. Click here to join now