Shane Zavodnik

Environmental Scientist at Northeast Technical Services, Inc.
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Contact Information
us****@****om
(386) 825-5501
Location
Hoyt Lakes, Minnesota, United States, US

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Experience

    • United States
    • Environmental Services
    • 1 - 100 Employee
    • Environmental Scientist
      • Jun 2016 - Present

      Ground Water Well and Surface Water Monitoring through practice of well stabilization MPCA SOP method. NPDES permit experienced- required sampling for site specific waterways, wells and surface water locations HAZWOPER certified MSHA Certified Ground Water Well and Surface Water Monitoring through practice of well stabilization MPCA SOP method. NPDES permit experienced- required sampling for site specific waterways, wells and surface water locations HAZWOPER certified MSHA Certified

    • United States
    • Chemical Manufacturing
    • 700 & Above Employee
    • Territory Manager
      • Jun 2014 - Jun 2016

    • United States
    • Government Administration
    • 700 & Above Employee
    • Chemist/Biologist
      • Aug 2012 - Mar 2014

      Assisting as a chemist in the Ecosystem and Assessment Research Branch: Collection of sediment, vegetation, and larval fishing for possibly new invasive species introduction. Analyzing water samples taken from the Great Lakes as well as other surrounding waters for analysis of key characteristic features. Assisting as a biologist, collecting benthic samples from waters possibly infested with newly introduced invasive species. Collecting zooplankton and larval fish samples from the Great Lakes to investigate known and unknown aquatic species. Show less

    • United States
    • Government Administration
    • 700 & Above Employee
    • Research Biologist
      • Aug 2011 - Jan 2012

      Genotypic sexing using PCR and gel electrophoresisMeasuring unknown concentrations of vitellogenin in a serum sample using Enzyme-linked immunosorbent assay (ELISA); Biologist. Assist and coordinate in developing a standardized bioassay (Larval Amphibian Growth and Development Assay) under the Endocrine Disruptor Screening Program with amphibian model species Xenopus sp. To investigate effects of chemical exposure on the hypothalamic-pituitary-gonadal and thyroidal axes by examining key maturation endpoints from an embryonic stage to an adult. Followed up and examined post tissue collection indicative to high sensitivity environmental changes.Related DutiesFMI pump ratio calibration and maintenancePeristaltic pump calibration, refurbishing, and maintenance on mechanical issuesDiluents and toxicant calculationsMaintaining Target Chemical concentrations within and in vivo exposure: 17-beta-Trenbolone, Prochloraz, and 4-tert-Octylohenol exposuresDaily record keeping and statistical measurements of physiological eventsDissection and extraction of particular organs for hormone analysis Show less

    • Biologist
      • Aug 2010 - Aug 2011

      Biologist. Assisted in development and validation of an assay that was capable of identifying the adverse consequences of exposure to endocrine disrupting chemicals in amphibians (Larval Amphibian Growth and Development Assay). Performed tissue collections, DNA extractions, Amplified Fragment Length Polymorphism analysis for genetic sex determination. Planned tissue sampling methods, organized sample handling and care. Optimized and wrote and operating procedure for an in-house X. laevis genotyping protocol. Show less

    • United States
    • Construction
    • 1 - 100 Employee
    • Environmental Biologist
      • Apr 2011 - Sep 2011

      Environmental Biologist. Collected water samples at beaches and rivers for Fecal Indicating Bacteria (FIB) and pathogen analyses in order to analyze and assess the effects of water quality characteristics due to industrialization and agriculture in Duluth, Minnesota. Duties and Accomplishments Knowledge and use of a HYDROLAB; daily monitoring of each beach site for pH, conductivity, dissolved oxygen, chlorophylls, and turbidity Daily water sample collection through proper field collection protocol Assembled multiple weather stations Maintenance and operation of automated sampling equipment Daily record taking of wave height, type of weather, nearby debris, animal activity, and human activity Maintained QA/QC throughout the study EPA Quality Assurance and Quality Control trained Kept daily records of solar and UV radiation, wind direction, precipitation, humidity and temperature Experience in writing scientific articles for peer review Show less

    • United States
    • Higher Education
    • 700 & Above Employee
    • Research Assistant
      • Dec 2009 - May 2010

      Researched to find a simpler purification method of Polyphenol Oxidase via Ipomoea batatas (sweet potato) using non-denaturing gel electrophoresis isolation. Planned an array of purification methods to implement while testing different assay methods. Assisted in creating a standardized assay for protein isolation and to analyze purity using column chromatography and absorption spectrometry. Researched to find a simpler purification method of Polyphenol Oxidase via Ipomoea batatas (sweet potato) using non-denaturing gel electrophoresis isolation. Planned an array of purification methods to implement while testing different assay methods. Assisted in creating a standardized assay for protein isolation and to analyze purity using column chromatography and absorption spectrometry.

Education

  • The College of St. Scholastica
    Bachelor of Arts (BA), Biology/Biological Sciences, General
    2007 - 2010
  • The College of St. Scholastica
    Bachelor of Science (BS), Biochemistry
    2007 - 2010
  • The College of St. Scholastica
    Bachelor of Science, Biochemistry
  • Mesabi Range Community and Technical College
    Associate of Arts (AA), Biology/Biological Sciences, General
    2004 - 2006

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