Shainnel Eans
Research Technician at Torrey Pines Institute for Molecular Studies- Claim this Profile
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Spanish Limited working proficiency
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Japanese Limited working proficiency
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Bio
Experience
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Torrey Pines
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United States
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Biotechnology Research
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1 - 100 Employee
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Research Technician
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Mar 2010 - Present
SUMMARY: Primary researcher of neurological and behavior-pharmacological studies using C57BL/6J mice, GT-tg Bigenic mice, and Mu/Kappa-opioid receptor knockout mice. TASKS PERFORMED: Methods of drug administration (in mice): Intraperitoneal, Sub-cutaneous, Intracerebroventricular, Oral gavage, Insufflation, Intravenous, Intraplantar, Intranasal injections Pain assays: Tail-flick, Hargraeves, Von Frey, Acetic acid stretching test, Intraplantar Capsaisin/Formalin testing Anxiety/Depression assays: Elevated plus maze, Marble-burying, Forced swim stress, Tail-suspension, PPI/Acoustic startle reflex, Open field, Social defeat stress studies (using dominant aggressors as the stimulus) Learning & Memory assays: Novel object recognition, Surgeries & Other: CCI (in CD-1 mice), Ovarectomy, Cycling of female mice Dissections (mice): Perfusions and Tissue collections of Brain, Liver, Kidney, Small & Large Intestines, Olfactory nerves, blood, etc. Drug liability testing: Conditioned-place preference, Oxymax/CLAMS (for measuring respiration & locomotor activity), Rotorod, Diuresis, Intestinal motility measurements Mouse colony maintenance: C57BL/6J mice, CD-1 Mice, GT-tg bigenic mice, Mu/Kappa opioid receptor knockout mice Show less
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Research Technician
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2007 - 2009
SUMMARY: Primary researcher of insecticide-resistance genes and gene-expression studies in Culex nigripalpus Theobald mosquito populations. TASKS PERFORMED: Molecular techniques: DNA/RNA extractions, PCR & RT-PCR, Agarose gel electophoresis, Cloning, Sequencing, and Sequencing analysis Primary researcher of insecticide-resistance genes and their expression in Culex nigripalpus mosquito populations Dissections (mosquitoes): Midgut, Head, Thorax, Abdomen Other assays: Bottle bioassay Mosquito colony maintenance: Culex nigripalpus Show less
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University of Florida
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United States
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Higher Education
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700 & Above Employee
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Volunteer
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2006 - May 2007
TASKS PERFORMED: Animal husbandry/ Colony maintenance: Singing mice and Prairie voles Researcher of neurological behavior (social learning and memory) of prairie voles (Microtus ochrogaster). (Was placed in charge of the behavioral portion of a main project) TASKS PERFORMED: Animal husbandry/ Colony maintenance: Singing mice and Prairie voles Researcher of neurological behavior (social learning and memory) of prairie voles (Microtus ochrogaster). (Was placed in charge of the behavioral portion of a main project)
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Education
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University of Florida
Master of Science (MS), Entomology -
University of Florida
Bachelor of Science (BS), Zoology/Animal Biology -
University of Florida
Minor, East Asian Languages, Literatures, and Linguistics