Experience

Affimed

• Germany
• Biotechnology Research
• 100 - 200 Employee

• Research Associate

  • May 2022 - Present

  • Developed in-house procedure for large-scale immune cell isolation and cultivation for downstream applications. Initiated development of novel cell products and testing therapeutic efficacy in combination with innate cell engagers. • Collaborate with CROs for further drug development.

• Senior Technician

  • Jan 2021 - May 2022

  • Establishing a new Translational Immunobiology lab within the company while introducing new devices and technologies that have been implemented to expand the understanding of ICE®s.• Designing, optimizing, and reporting of studies using primary human immune cells for further characterization and functional profiling with cancer cell lines for internal programs and CROs.



DKFZ German Cancer Research Center

• Germany
• Research Services
• 700 & Above Employee

• Research Scientist

  • Jun 2019 - Dec 2020

  • Coordinating a small group of researchers to develop cellular assays and high-throughput screening methods for applications with novel small molecules designed specific inhibitory profiles to better characterize possible therapeutic targets using in vitro, ex vivo, and in vivo models.• Designing and executing high-throughput drug screens that test the synergistic potential of clinically tested cancer therapies and inhibitors of known pathways and novel biomarkers conferring drug resistance in human cell lines.



EMBL

• Germany
• Research Services
• 700 & Above Employee

• Technical Specialist

  • Aug 2016 - Jun 2019

  • Responsible for basic lab maintenance procedures.• Responsible for breeding and maintaining transgenic mouse lines.• Isolating cell lineages in the mammary epithelium via FACS to study the contribution of mammarygland stem cells and progenitors to tumor initiation and recurrence.• Utilize a 3D culture system of primary mouse mammary epithelial cells to study cancer-initiating cellsin tumor recurrence.• Employing molecular biology techniques, histopathological analysis, and live cell imagingto investigate organotypic 3D cell culture systems and the corresponding mouse models.• Single cell sequencing analysis of regressed organoids to study the modified genetic signature of minimal residual disease compared to normal cells in culture.



University of North Carolina at Chapel Hill School of Medicine

• United States
• Higher Education
• 700 & Above Employee

• Research Technician

  • Jan 2015 - Jul 2016

  Responsible for basic lab maintenance procedures.• Generating novel AAV genomes with additional nucleolar localization sequences usingtraditional and Gibson cloning and characterizing resultant phenotypes.• Assaying virus production, stability, and infectivity in immortalized cell lines usingbiochemical methods (triple transfection, glycerol gradient virus isolation, Western blot, etc.) andfluorescent microscopy to determine protein accumulation and capsid assembly.• Assisting with a chapter publication for Methods in Molecular Biology on designing novelAAV vectors with cardiac tropism.



North Carolina State University

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Researcher

  • May 2013 - Dec 2014

  • Responsible for basic lab maintenance procedures.• Assisted in genome-wide association study of the influence of male-mediated effects on crossover rates in Drosophila melanogaster using visible genetic markers to assay variation in recombination.• Generating transgenic bacteria containing a null mutation in the promoter region of a vital gene in Drosophila CNS development to be injected into embryos that were propagated and whose progeny were used for antibody staining and visualization of inhibited development using confocal microscopy.• Virgin Drosophila females selected from a wild-type stock were exposed to prolonged heat shock before mating to a recessive stock of males in order to assess the impact of environmental stress on rates of meiotic recombination.

• Undergraduate Researcher

  • Aug 2012 - Dec 2014

  • Responsible for basic lab maintenance procedures.• Generation of transgenic Agrobacterium lines using recombineering protocols for Arabidopsis transformation to map gene expression in the auxin biosynthetic pathway visualized with fluorescent tags using fluorescent microscopy.• Utilization of traditional and confocal microscopy to analyze root degeneration and hormone over-expression in Arabidopsis seedlings due to environmental stress.



Bruster's Real Ice Cream

• United States
• Food and Beverage Services
• 200 - 300 Employee

• Crew Leader

  • Jun 2010 - Dec 2012