Postdoctoral Fellow

• Aug 2020 - Present

- Construct design, cloning, purification, quantification, primer design, PCR, site-directed mutagenesis, plasmid preparation and analyzing DNA sequencing data- Buffer/media/reagent preparation, aseptic techniques, cell culture techniques including isotopic labelling of proteins- Small scale and large scale over expression of recombinant proteins in E. coli- Protein purification using centrifugation, affinity chromatography (His-tag, GST tag), size exclusion chromatography, ion exchange chromatography using FPLC (AKTA system-UNICORN 7)- RNA transcription and purification- Studying protein-DNA interactions, protein-RNA interactions and protein-peptide interaction studies using Nuclear Magnetic Resonance (NMR) Spectroscopy (Varian 900 MHz Direct Drive NMR system, Varian INOVA 600 MHz, and Bruker Avance Neo 600 MHz)- Gel electrophoresis (SDS-PAGE/Native-PAGE), Electrophoresis Shift Mobility Assays (EMSA), Western blotting- Design experiments, method development, process optimization, process documentation, and troubleshoot for multi-step protein purification protocols- Maintain organized laboratory notebooks- Review and update documentation for existing laboratory/technical procedures with necessary changes- Operate, maintain, and troubleshoot of AKTA FPLC system and other instrumentation- Prepare, evaluate, analyze and present data at scientific conferences and writing of independent research data for scientific journals - Handle hazardous waste - Training and mentoring graduate and undergraduate students

Professional Research Assistant

• Mar 2020 - Aug 2020

Visiting Research Assistant

• Aug 2019 - Mar 2020

Graduate Research Assistant

• May 2018 - Aug 2019

- Purification & quantification of DNA, primer design, PCR, site-directed mutagenesis, plasmid preparation and analyzing DNA sequencing data- Small scale and large scale over expression of recombinant proteins in E. coli- Protein purification using centrifugation, affinity chromatography, size exclusion chromatography, ion exchange chromatography using FPLC - Studying Protein-DNA interactions and protein-RNA interactions studies using Nuclear Magnetic Resonance (NMR) Spectroscopy (Bruker Avance II 800 MHz) and ForteBio Octet RED96 Biolayer Interferometry (BLI) System - Gel electrophoresis (SDS-PAGE/Native-PAGE) and Electrophoresis Shift Mobility Assays (EMSA)- Maintain organized laboratory notebooks- Prepare, evaluate, analyze and present data at scientific conferences and writing of independent research data for scientific journals - Training and mentoring graduate and high school students

Graduate Research And Teaching Assistant

• Aug 2015 - May 2019

- Teaching Assistant for Principles Chemistry II class & laboratory from Fall 2015-Spring 2018- Over expression of recombinant proteins in E. coli and purification- Hands on experince with Fast protein liquid chromatography (FPLC) – Size Exclusion chromatography (SEC) - PCR, Plasmid preparation and analyzing DNA sequencing data- Gel electrophoresis (SDS PAGE)- Study of protein-metal interactions studies and protein activity assays using Fluorescence Anisotropy (FA), High-performance liquid chromatography (HPLC), Ultraviolet-Visible Spectroscopy (UV-Vis) and Inductively coupled plasma atomic emission spectroscopy (ICP-OES)

Laboratory Demonstrator/Teaching Assistant

• Feb 2014 - Jan 2015

Teaching Assistant for - Basic Analytical Chemistry Laboratory (In charge)- General Chemistry and Basic Analytical Chemistry- Molecular Biochemistry Laboratory- Advanced Biochemistry (I) - Organic, Analytical and Synthetic Chemistry Laboratory (In-charge of synthetic chemistry practicals)- Environmental and Agricultural Biochemistry Laboratory- Functional Biochemistry Laboratory- Functional Biochemistry- Advanced Biochemistry (II) Teaching Assistant for - Basic Analytical Chemistry Laboratory (In charge)- General Chemistry and Basic Analytical Chemistry- Molecular Biochemistry Laboratory- Advanced Biochemistry (I) - Organic, Analytical and Synthetic Chemistry Laboratory (In-charge of synthetic chemistry practicals)- Environmental and Agricultural Biochemistry Laboratory- Functional Biochemistry Laboratory- Functional Biochemistry- Advanced Biochemistry (II)