Sara Victoria Coyotzi Alcaraz

Analytical Scientist at Ascend Gene & Cell Therapies
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Contact Information
us****@****om
(386) 825-5501
Location
Barnet, England, United Kingdom, GB
Languages
  • Spanish Native or bilingual proficiency
  • English Native or bilingual proficiency
  • French Elementary proficiency

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Experience

    • United Kingdom
    • Biotechnology Research
    • 1 - 100 Employee
    • Analytical Scientist
      • Jul 2022 - Present

    • United Kingdom
    • Biotechnology
    • 100 - 200 Employee
    • Analytical Scientist
      • Nov 2021 - Jun 2022

    • Netherlands
    • Biotechnology Research
    • 700 & Above Employee
    • IVD Assay Development Scientist
      • Jan 2018 - Oct 2021

      I design, lead and manage In Vitro Diagnostics (IVD) oncology assay studies.I optimise the performance and assess the robustness of IVD biomarker assays using design of experiments.I assist the automation of sequencing workflows on Hamilton liquid handling workstations. I execute NGS workflows from tumour DNA/RNA on proprietary sequencers and Illumina’s NextSeq 550.Responsible for procurement of materials, samples and management of inventory. I support the maintenance and safety of laboratories within the sequencing suite. Document reviewer, document writer and process trainer in GMP environment.

    • Higher Education
    • 700 & Above Employee
    • Visiting Scientist - Cell Culture & Cell Based Assays
      • Sep 2017 - Dec 2017

      Using cell culture and cell banking for cell line development.Using cell based assays to evaluate cellular viability and activity. Using cell culture and cell banking for cell line development.Using cell based assays to evaluate cellular viability and activity.

    • Canada
    • Higher Education
    • 700 & Above Employee
    • Research Analyst - Molecular Biology & Bioinformatics
      • May 2016 - Jun 2017

      Optimised DNA extraction methods for challenging low-biomass matrices.Bioinformatics and experimental primer efficiency studies to achieve full PCR coverage of microorganisms. Illumina sequencing for the microbial characterisation of diverse environments.Developed a pipeline for automated processing and visualisation of sequencing data using R software.Developed and validated an analytical method for the selective recovery of DNA based on density.Established a culture collection and associated preservation protocols.Statistical data analysis and preparation of scientific manuscripts for publication.

    • Education Administration Programs
    • 1 - 100 Employee
    • Documentation Supervisor - Regulatory Compliance
      • Sep 2015 - Apr 2016

      Analysed drug dossiers to assess compliance with local regulatory requirements for drug registration.Guaranteed the accurateness of technical data and reports for pharmaceutical and engineering companies. Analysed drug dossiers to assess compliance with local regulatory requirements for drug registration.Guaranteed the accurateness of technical data and reports for pharmaceutical and engineering companies.

    • United States
    • Research Services
    • 700 & Above Employee
    • Experimental Officer 3 - Protein & Antibody Electrophoresis
      • Nov 2014 - Jun 2015

      Performed SDS-PAGE and capillary electrophoresis for protein characterisation and method validation.Prepared documentation for QA audits and addressed audit observations for data approval.Liaised with lead scientists, supervisors and analysts for timely delivery of results for specific studies.Ensured availability of reagents and consumables for drug testing. Performed SDS-PAGE and capillary electrophoresis for protein characterisation and method validation.Prepared documentation for QA audits and addressed audit observations for data approval.Liaised with lead scientists, supervisors and analysts for timely delivery of results for specific studies.Ensured availability of reagents and consumables for drug testing.

    • Canada
    • Higher Education
    • 700 & Above Employee
    • Graduate Research Assistant - Molecular Biology, Chemistry & Chromatography
      • Jan 2012 - Jun 2014

      Designed and conducted short and long term experiments.Analysed chemical and biological data.Designed analytical methodologies and evaluated equipment performance.Prepared manuscripts and technical reports.Trained personnel in gas chromatography and molecular biology techniques.Organised and presented results to stakeholders.

    • Teaching Assistant - Laboratory Demonstrator
      • 2012 - 2014

      Taught laboratories: Fundamentals of Microbiology, Introduction to Applied Microbiology, and Microbial Ecology and Diversity.Demonstrated microbiology and molecular biology laboratory techniques to undergraduate students.Supervised and mentored students in the laboratory.Evaluated student performance and reports.

    • Mexico
    • Pharmaceutical Manufacturing
    • 300 - 400 Employee
    • Research & Development Chemist - Protein & Antibody Chromatography
      • Jul 2011 - Dec 2011

      Developed HPLC and UPLC techniques for protein purification and characterisation.Executed UPLC for glycan characterisation.Prepared technical reports.

    • Quality Control Chemist - Microbiology & Immunoassays
      • Jan 2010 - Jun 2011

      Executed and validated microorganism and endotoxin detection methods for the manufacturing water, bulk materials and intermediate and final products.Executed ELISA for product potency determination and quantification .Executed air quality monitoring for the manufacturing facility. Designed preliminary methods for cleaning validation.

    • Trainee
      • Sep 2008 - Dec 2009

      Pursued specific objectives within given deadlines. Main assignments: Developed bacteria and fungi preservation techniques. Created the methodology and system to control the training requirements of employees.Promoted the migration of the manufacturing water quality to water for injection.

Education

  • University of Waterloo
    Master of Science (M.Sc.), Microbiology and Molecular Biology
    2012 - 2014
  • Instituto Politécnico Nacional
    Bachelor of Science (B.Sc.), Bacteriologist Parasitologist Chemist
    2003 - 2008

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